Correction-free remotely scanned two-photon in vivo mouse retinal imaging

查看全文 作　　者：Adi Schejter Bar-[1]Noam;Nairouz [1]Farah;Shy [1]Shoham

机构地区：[1]Faculty of Biomedical Engineering,Technion–Israel Institute of Technology,Kiryat HaTechnion,Haifa 32000,Israel

出　　处：《Light(Science & Applications)》索引2016年第5卷第1期,共9页

基　　金：supported by the European Research Council(ERC)under the European Union’s Horizon 2020 research and innovation program,#641171;by the Israel Science Foundation(ISF)#1725/13;by a Gutwirth Fellowship to A.S. 摘　　要：Non-invasive fluorescence retinal imaging in small animals is an important requirement for an array of translational vision applications.The in vivo two-photon imaging of the mouse retina may enable the long-term investigation of the structure and function of healthy and diseased retinal tissue.However,to date,this has only been possible using relatively complex adaptive-optics systems.Here,the optical modeling of the murine eye and of the imaging system is used to achieve correction-free two-photon microscopy through the pupil of a mouse eye to yield high-quality,optically sectioned fundus images.By remotely scanning the focus using an electronically tunable lens,high-resolution three-dimensional fluorescein angiograms and cellular-scale images are acquired,thus introducing a correction-free baseline performance level for two-photon in vivo retinal imaging.Moreover,the system enables functional calcium imaging of repeated retinal responses to light stimulation using the genetically encoded indicator,GCaMP6s.These results and the simplicity of the new add-on optics are an important step toward several structural,functional,and multimodal imaging applications that will benefit from the tight optical sectioning and the use of near-infrared light. 关键词：ANGIOGRAPHY eye model functional calcium imaging in vivo OPTOGENETICS two-photon microscopy

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Correction-free remotely scanned two-photon in vivo mouse retinal imaging

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