On-chip 3D rotation of oocyte based on a vibration-induced local whirling flow

查看全文 作　　者：Takeshi [1]Hayakawa;Shinya [1]Sakuma;Fumihito [1]Arai

机构地区：[1]Department of Micro-Nano Systems Engineering,Nagoya University,Nagoya-City,Aichi-Pref.,JAPAN

出　　处：《Microsystems & Nanoengineering》索引2015年第1卷第1期,共9页

基　　金：This study was financially supported by Grant-in-Aid for JSPS Fellows Number 13J03580;Grant-in-Aid for Scientific Research on Innovative Areas(No.23106002);(No.26630094). 摘　　要：We propose a novel on-chip 3D cell rotation method based on a vibration-induced flow.When circular vibration is applied to a microchip with micropillar patterns,a highly localized whirling flow is induced around the micropillars.The direction and velocity of this flow can be controlled by changing the direction and amplitude of the applied vibration.Furthermore,this flow can be induced on an open chip structure.In this study,we adopted a microchip with three micropillars arranged in a triangular configuration and an xyz piezoelectric actuator to apply the circular vibration.At the centre of the micropillars,the interference of the vibration-induced flows originating from the individual micropillars induces rotational flow.Consequently,a biological cell placed at this centre rotates under the influence of the flow.Under three-plane circular vibrations in the xy,xz or yz plane,the cell can rotate in both the focal and vertical planes of the microscope.Applying this 3D cell rotation method,we measured the rotational speeds of mouse oocytes in the focal and vertical planes as 63.7±4.0°s^(−1) and 3.5±2.1°s^(−1),respectively.Furthermore,we demonstrated the transportation and rotation of the mouse oocytes and re-positioned their nuclei into a position observable by microscope. 关键词：lab on a chip cell manipulation vibration-induced flow acoustic streaming

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On-chip 3D rotation of oocyte based on a vibration-induced local whirling flow

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