AFM-compatible microfluidic platform for affinity- based capture and nanomechanical characterization of circulating tumor cells

查看全文 作　　者：Muhammedin [1]Deliorman;Farhad [2]K.Janahi;Pavithra [1]Sukumar;Ayoub [1,3]Glia;Roaa [1]Alnemari;Samar [4]Fadl;Weiqiang [3,5]Chen;Mohammad [1,3]A.Qasaimeh

机构地区：[1]Division of Engineering,New York University Abu Dhabi,P.O.Box 129188,Abu Dhabi,UAE;[2]Mohammed Bin Rashid University of Medicine and Health Sciences,P.O.Box 505055,Dubai,UAE;[3]Department of Mechanical and Aerospace Engineering,New York University,New York,NY 10003,USA;[4]Division of Science,New York University Abu Dhabi,P.O.Box 129188,Abu Dhabi,UAE;[5]Department of Biomedical Engineering,New York University,New York,NY 10003,USA

出　　处：《Microsystems & Nanoengineering》索引2020年第6卷第1期,共15页

基　　金：This work was supported by a Seed Grant(AJF201523)from the Al Jalila Foundation,Dubai,UAE;the 2017 NYU Abu Dhabi Research Enhancement Fund,UAE;the Terry Fox Foundation’s International Run Program,Vancouver,Canada. 摘　　要：Circulating tumor cells(CTCs)carried by the patient’s bloodstream are known to lead to the metastatic spread of cancer.It is becoming increasingly clear that an understanding of the nanomechanical characteristics of CTCs,such as elasticity and adhesiveness,represents advancements in tracking and monitoring cancer progression and metastasis.In the present work,we describe a combined microfluidic–atomic force microscopy(AFM)platform that uses antibody–antigen capture to routinely isolate and nanomechanically characterize CTCs present in blood samples from prostate cancer patients.We introduce the reversible assembly of a microfluidic device and apply refined and robust chemistry to covalently bond antibodies onto its glass substrate with high density and the desired orientation.As a result,we show that the device can efficiently capture CTCs from patients with localized and metastatic prostate cancer through anti-EpCAM,anti-PSA,and anti-PSMA antibodies,and it is suitable for AFM measurements of captured intact CTCs.When nanomechanically characterized,CTCs originating from metastatic cancer demonstrate decreased elasticity and increased deformability compared to those originating from localized cancer.While the average adhesion of CTCs to the AFM tip surface remained the same in both the groups,there were fewer multiple adhesion events in metastatic CTCs than there were in their counterparts.The developed platform is simple,robust,and reliable and can be useful in the diagnosis and prognosis of prostate cancer as well as other forms of cancer. 关键词：ROUTINE diagnosis ELASTICITY

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AFM-compatible microfluidic platform for affinity- based capture and nanomechanical characterization of circulating tumor cells

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