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High-density SNP-based genetic maps for the parents of an outcrossed and a selfed tetraploid garden rose cross, inferred from admixed progeny using the 68k rose SNP array

查看全文 作  者:Mirjana [1,3]Vukosavljev;Paul [1]Arens;Roeland E [1]Voorrips;Wendy P C van't [1]Westende;G D [1]Esselink;Peter M [1]Bourke;Peter [2]Cox;W Eric van de [1]Weg;Richard G F [1]Visser;Chris [1]Maliepaard;Marinus J M [1]Smulders 高影响力作者 机构地区:[1]Wageningen UR Plant Breeding,Wageningen University&Research,Wageningen,NL-6700 AJ,The Netherlands;[2]Roath BV,Eindhoven,The Netherlands;[3]Pheno Geno Roses D.O.O.Bulevar oslobođenja 65a,21000 Novi Sad,Serbia高影响力机构 出  处:《Horticulture Research》索引2016年第3卷第1期,共8页高影响力期刊 基  金:This research was partly supported by the TTI Green Genetics projects‘Hyperrose’and‘Polyploids’and by the TKI Polyploids project H263(BO-26.03-002-001). 摘  要:Dense genetic maps create a base for QTL analysis of important traits and future implementation of marker-assisted breeding.In tetraploid rose,the existing linkage maps include<300 markers to cover 28 linkage groups(4 homologous sets of 7 chromosomes).Here we used the 68k WagRhSNP Axiom single-nucleotide polymorphism(SNP)array for rose,in combination with SNP dosage calling at the tetraploid level,to genotype offspring from the garden rose cultivar‘Red New Dawn’.The offspring proved to be not from a single bi-parental cross.In rose breeding,crosses with unintended parents occur regularly.We developed a strategy to separate progeny into putative populations,even while one of the parents was unknown,using principle component analysis on pairwise genetic distances based on sets of selected SNP markers that were homozygous,and therefore uninformative for one parent.One of the inferred populations was consistent with self-fertilization of‘Red New Dawn’.Subsequently,linkage maps were generated for a bi-parental and a self-pollinated population with‘Red New Dawn’as the common maternal parent.The densest map,for the selfed parent,had 1929 SNP markers on 25 linkage groups,covering 1765.5 cM at an average marker distance of 0.9 cM.Synteny with the strawberry(Fragaria vesca)genome was extensive.Rose ICM1 corresponded to F.vesca pseudochromosome 7(Fv7),ICM4 to Fv4,ICM5 to Fv3,ICM6 to Fv2 and ICM7 to Fv5.Rose ICM2 corresponded to parts of F.vesca pseudochromosomes 1 and 6,whereas ICM3 is syntenic to the remainder of Fv6. 关 键 词:breeding LINKAGE DOSAGE
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