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Evaluation of Multidrug Resistant Loop-mediated Isothermal Amplification Assay for Detecting the Drug Resistance of Mycobacterium tuberculosis

查看全文 作　　者：LIU Chun [1]Fa;SONG Yi [2]Meng;HE [3]Ping;LIU Dong [4]Xin;HE Wen [3]Cong;LI Yan [2]Ming;ZHAO Yan [1]Lin 高影响力作者 机构地区：[1]National Center for Tuberculosis Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;[2]National Center of Gerontology,Beijing Hospital,Beijing 100730,China;[3]National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;[4]National Clinical Research Center for Infection Disease,Shenzhen Third People's Hospital,Shenzhen 518112,Guangdong,China高影响力机构 出　　处：《Biomedical and Environmental Sciences》索引2021年第34卷第8期,共7页高影响力期刊 基　　金：the Chinese Center for Disease Control and Prevention project[59911905 and 59911916]. 摘　　要：Objective To evaluate multidrug resistant loop-mediated isothermal amplification(MDR-LAMP)assay for the early diagnosis of multidrug-resistant tuberculosis and to compare the mutation patterns associated with the rpoB,katG,and inhA genes at the Chinese Center for Disease Control and Prevention.Methods MDR-LAMP assay was evaluated using 100 Mycobacterium tuberculosis(Mtb)isolates obtained from the National Reference Laboratory for Tuberculosis in China.Phenotypic resistance to isoniazid and rifampicin and whole-genome sequencing served as reference standards.Results The sensitivity,specificity,positive predictive value(PPV),and negative predictive value(NPV)of MDR-LAMP were 85.5%,93.6%,96.7%,and 74.4%for the detection of resistance to isoniazid and rifampicin,respectively,and 80.5%,92.3%,98.6%,and 41.4%for the detection of Mtb cultured from smear-positive sputum samples,respectively.When DNA sequencing was used as the reference standard,the sensitivity,specificity,PPV,and NPV of MDR-LAMP were 93.1%,92.3%,97.2%,and 82.8%for the detection of katG and inhA gene mutations,respectively,and 89.1%,88.9%,93.4%,and 81.1%for the detection of rpoB gene mutation,respectively.Conclusion MDR-LAMP is a rapid and accessible assay for the laboratory identification of rifampicin and isoniazid resistance of Mtb isolates. 关 键 词：MDR-LAMP MYCOBACTERIUM TUBERCULOSIS DIAGNOSTIC method
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