Rapid Internal Control Reference Recombinase-Aided Amplification Assays for EBV and CMV Detection

查看全文 作　　者：GAO [1,2,3]Yuan;TIE Yan [3]Qing;ZHAO Lin [4]Qing;TAN [1,3]He;DING [3]Nan;DING Ya [4]Xin;GUO [4]Qi;ZHANG Rui [2]Qing;WANG Jin [1,2]Rong;CHEN Zi [2,5]Wei;FAN Guo [2]Hao;SHEN Xin [2]Xin;FENG Zhi [3]Shan;MA Xue [2,6]Jun

机构地区：[1]Hebei Medical University,Shijiazhuang 050031,Hebei,China;[2]NHC Key Laboratory of Medical Virology and Viral Diseases,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;[3]Hebei General Hospital,Shijiazhuang 050070,Hebei,China;[4]Laboratory of Virology,Beijing Key Laboratory of Etiology of Viral Diseases in Children,Capital Institute of Pediatrics,Beijing 100020,China;[5]The Third Xiangya Hospital of Central South University,Changsha 410013,Hunan,China;[6]Center for Biosafety Mega-Science,Chinese Academy of Sciences,Wuhan 430071,Hubei China

出　　处：《Biomedical and Environmental Sciences》索引2021年第34卷第8期,共6页

基　　金：the China Mega-Projects for Infectious Disease[grant no.2018ZX10711001,2017ZX10104001 and 2018ZX10713-002];the National Institute for Viral Disease Control and Prevention[grant no.2019HYDQNJJ03]. 摘　　要：Epstein-Barr virus(EBV)and cytomegalovirus(CMV),two of the most prevalent human herpesviruses,cause a wide spectrum of diseases and symptoms and are associated with serious health problem.In this study,we developed an internal control reference recombinase-aided amplification(ICR-RAA)assay for the rapid detection of EBV and CMV within 30 min.The assay had a sensitivity of 5 and 1 copies/test for EBV and CMV,respectively,with no cross reaction with other pathogens.In comparison with those of the commercial quantitative polymerase chain reaction(qPCR),the sensitivity of the EBV and CMV ICR-RAAs using extracted DNA was 93.33%and 84.84%,respectively;the specificity was 98.75%and 100.00%,respectively;and the Kappa values were 0.930 and 0.892(P<0.05),respectively.In comparison with those of qPCR,the sensitivity of the EBV and CMV ICR-RAAs using the DNA by thermal lysis was 72.22%and 80.00%,respectively;the specificity was 100.00%。 关键词：SPECIFICITY INTERNAL EBV

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Rapid Internal Control Reference Recombinase-Aided Amplification Assays for EBV and CMV Detection

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