Generation of a series of mutant lines resistant to imidazolinone by screening an EMS-based mutant library in common wheat

查看全文 作　　者：Zhuo [1]Chen;Zheng [2]Wang;Yanfang [1]Heng;Jian [2]Li;Jiawei [1]Pei;Ying [1]Cao;Xing Wang [2]Deng;Ligeng [1]Ma

机构地区：[1]College of Life Sciences,Beijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement,Capital Normal University,Beijing 100048,China;[2]School of Advanced Agricultural Sciences,Peking University,Weifang 261000,Shandong,China

出　　处：《The Crop Journal》索引2021年第9卷第5期,共9页

基　　金：financially supported by the National Key Research and Development Program of China (2017YFD0101001);Beijing Municipal Government Science Foundation (IDHT20170513);Peking University Institute of Advanced Agricultural Sciences。 摘　　要：The breeding of herbicide-resistant wheat varieties has helped control weeds in wheat fields economically and effectively.Imidazolinone (IMI) herbicides are popular as they have low toxicity in mammals,are effective at small doses,and exhibit broad-spectrum herbicidal action in the field.Therefore,the isolation and genetic and molecular characterization of IMI-resistant wheat mutants will enhance weed management in wheat fields.In the present study,352 IMI-resistant plants were isolated by genetic screening from a mutant pool prepared by EMS-based random mutagenesis.Cloning of the mutated genes from the IMI-resistant plants indicated that ten taals alleles had been isolated,and mutation in one of three Ta ALS homolog genes conferred IMI resistance,and such a mutation is a dominant trait.Further analysis showed that taals-d exhibited the greatest IMI resistance,whereas taals-b exhibited the weakest resistance to IMI among three homologous taals mutants.In terms of IMI resistance,the taals triple mutant was stronger than the taals double mutants,and the taals double mutants were stronger than the single mutants,indicating a dose-dependent effect of the Ta ALS mutation on IMI resistance in wheat.Biochemical analysis indicated that the mutation in Ta ALS increased the tolerance of Ta ALS to inhibition by IMI.Our work details the genetic and molecular characterization of als wheat mutants,provides a foundation for understanding IMI resistance and breeding wheat varieties with herbicide resistance,and indicates that genetic screening using a mutagenized pool is an effective and important means of breeding crops with additional desired agricultural traits. 关键词：WHEAT HERBICIDE Genetic screening Imidazolinone resistance Taals alleles

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Generation of a series of mutant lines resistant to imidazolinone by screening an EMS-based mutant library in common wheat

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