Warming moxibustion attenuates inflammation and cartilage degradation in experimental rabbit knee osteoarthritis

查看全文 作　　者：LIU [1]Di;WU [1]Yongli;LI [1]Chun;WANG [2]Minglei;MA [1]Xiaoxiu;LIU [3]Junwei;ZHANG [1]Yanling;YANG [1]Lei

机构地区：[1]Traditional Chinese Medicine and Traumatology,General Hospital of Ningxia Medical University,Yinchuan 750004,China;[2]Radiology Department,General Hospital of Ningxia Medical University,Yinchuan 750004,China;[3]Department of Acu-moxibustion and Tuina,Ningxia Medical University,Yinchuan 750004,China

出　　处：《Journal of Traditional Chinese Medicine》索引2021年第41卷第6期,共9页

基　　金：Supported by National Natural Science Foundation of China:the Treatment Mechanism of Koa with Zhen Shamazhunjiu and Regulation Effect of Zhen Sha Serum on Upa were Studied on the Basis of Upa System(No.81560814);the Mechanism of Warming Moxibustion on Rabbit Koa Subchondral Bone was Studied on the Basis of OPG/RANKL/RANK System(No.81760891);the Third Batch of Ningxia Youth Talents Supporting Program(No.TJGC2018029);the Second Batch of Science and Technology Leading Talents Project of Ningxia Hui Autonomous Region(No.KJJ201610)。 摘　　要：OBJECTIVE:To explore the molecular mechanism of warming moxibustion(WM)in knee osteoarthritis(KOA).METHODS:The knee joints of 40 New Zealand rabbits were placed in a plaster cast in an extended position to establish a KOA model.The animals were randomly divided into four groups:the control group,model group,WM group,and diclofenac(DF)group.Hematoxylin-eosin staining and the modified Mankin score were applied to evaluate the histopathological changes.Chondrocyte apoptosis was determined by the terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL)assay.Western blotting and real-time quantitative polymerase chain reaction were performed to measure the expression of interleukin-1β(IL-1β),prostaglandin E receptor 3(PTGER3),a disintegrin-like and metalloproteinase with thrombospondin type-1 motifs-5(ADAMTS-5),matrix metalloproteinase-13(MMP-13),and C-terminal telopeptides of collagen typeⅡ(CTX-Ⅱ)in cartilage tissues of the different groups.The concentrations of IL-1β,PTGER3,and CTX-Ⅱin serum were detected by the enzyme-linked immunosorbent assay.RESULTS:Rabbits with KOA in the WM and DF groups showed significantly reduced cartilage erosion and Mankin scores,compared with the untreated rabbits.The number of TUNEL-positive cells observed in the WM group was much fewer than that in the model group.The expression of PTGER3,MMP-13,CTX-Ⅱ,IL-1β,and ADAMTS-5 in cartilage tissues was remarkably downregulated following therapy with WM and DF.Moreover,a marked reduction was observed in the serum levels of IL-1β,PTGER3,and CTX-Ⅱin the WM and DF groups.CONCLUSION:WM exerts favorable therapeutic effects on articular injuries of KOA by regulating the expression of inflammatory and cartilage degradation-related cytokines. 关键词：warming moxibustion osteoarthritis,knee INFLAMMATION cartilage degradation

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Warming moxibustion attenuates inflammation and cartilage degradation in experimental rabbit knee osteoarthritis

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