Anti-microtubule activity of the traditional Chinese medicine herb Northern Ban Lan(Isatis tinctoria)leads to glucobrassicin

查看全文 作　　者：Pingyin [1,4]Guan;Jianning [1]Zhou;Sergey [2,5]Girel;Xin [1]Zhu;Marian [1]Schwab;Kunxi [1]Zhang;Qiyan Wang-[3,6]Müller;Laurent [2]Bigler;Peter [1]Nick

机构地区：[1]Molecular Cell Biology,Botanical Institute,Karlsruhe Institute of Technology,Fritz-Haber-Weg 4,Karlsruhe 76131,Germany;[2]Department of Chemistry,University of Zürich,Winterthurerstr.190,CH-8057,Zurich,Switzerland;[3]Research Institute of Organic Agriculture FiBL,Ackerstrasse 113,CH-5070,Frick,Switzerland;[4]College of Horticulture,China Agricultural University,Beijing 100193,China;[5]Institute of Pharmaceutical Sciences of Western Switzerland,University of Geneva,Rue Michel-Servet 1,CH-1211,Geneva 4,Switzerland;[6]Swiss Chinese Herbal Medicine and Functional Food Innovation Center,Mainaustrasse 21,CH-8046,Zurich,Switzerland

出　　处：《Journal of Integrative Plant Biology》索引2021年第63卷第12期,共17页

基　　金：supported by fellowships from the China Scholarship Council。 摘　　要：Traditional Chinese medicine(TCM)belongs to the most elaborate and extensive systems of plant-based healing.The herb Northern Ban Lan(Isatis tinctoria)is famous for its antiviral and anti-inflammatory activity.Although numerous components isolated from I.tinctoria have been characterized so far,their modes of action have remained unclear.Here,we show that extracts from I.tinctoria exert antimicrotubular activity.Using time-lapse microscopy in living tobacco BY-2(Nicotiana tabacum L.cvBrightYellow2)cellsexpressing greenfluorescentprotein-tubulin,weuse activity-guided fractionation to screen out the biologically active compounds of I.tinctoria.Among 54 fractions obtained from either leaves or roots of I.tinctoria by methanol(MeOH/H_(2)O 8:2),or ethyl acetate extraction,one specific methanolic root fraction was selected,because it efficiently and rapidly eliminated microtubules.By combination of further purification with ultrahigh-performance liquid chromatography and high-resolution tandem mass spectrometry most of the bioactivity could be assigned to the glucosinolate compound glucobrassicin.Glucobrassicin can also affect microtubules and induce apoptosis in HeLa cells.In the light of these findings,the antiviral activity of Northern Ban Lan is discussed in the context of microtubules being hijacked by many viral pathogens for cell-to-cell spread. 关键词：anti-microtubule activity cytoskeleton glucobrassicin high-performance liquid chromatography(HPLC) high-resolution tandem mass spectrometry(HRMS/MS) Isatis tinctoria Northern Ban Lan traditional Chinese medicine(TCM)

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Anti-microtubule activity of the traditional Chinese medicine herb Northern Ban Lan(Isatis tinctoria)leads to glucobrassicin

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