Dietary supplementation with inulin improves lactation performance and serum lipids by regulating the rumen microbiome and metabolome in dairy cows

查看全文 作　　者：Yue [1,2]Wang;Xuemei [1]Nan;Yiguang [1]Zhao;Linshu [3]Jiang;Hui [1]Wang;Dengke [1]Hua;Fan [1]Zhang;Yapin [1]Wang;Jun [4]Liu;Junhu [2]Yao;Benhai [1]Xiong

机构地区：[1]State Key Laboratory of Animal Nutrition,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing,100193,China;[2]College of Animal Science and Technology,Northwest A&F University,Yangling,712100,China;[3]Beijing Key Laboratory for Dairy Cow Nutrition,Beijing University of Agriculture,Beijing,102206,China;[4]Langfang Academy of Agriculture and Forestry,Langfang,065000,China

出　　处：《Animal Nutrition》索引2021年第4期,共16页

基　　金：funded by the National Key R&D Program of China(Grant No.2019YFE0125600);Beijing Dairy Industry Innovation Team(bjcystx-ny-1)。 摘　　要：This study investigated the effects of inulin on rumen fermentation parameters,ruminal microbiome and metabolites,as well as lactation performance and serum indexes in dairy cows.Sixteen Holstein dairy cows with similar body conditions were randomly divided into 2 groups(n=8 per group),with inulin addition at 0 and 200 g/d percow.The experiment lasted for 6 weeks,including a 1-week adaptation period and a 5-week treatment period.At the end of the experimental period,the milk,serum and rumen fluid were sampled and analyzed.The microbiome and metabolome in the rumen fluid were analyzed via 16 S rRNA sequencing and untargeted metabolomics,respectively.The results showed that supplementation with inulin(200 g/d per cow)increased the milk yield(P=0.001),milk protein(P=0.032),lacto se rate(P=0.004)and proportion of saturated fatty acids(SFA)in milk(P<0.001),but decreased the proportion of unsaturated fatty acids(USFA)(P=0.041).Rumen pH(P=0.040)and the concentration of NH3-N(P=0.024)were decreased;however,acetate(P<0.001),propionate(P=0.003),butyrate(P<0.001)and lactic acid(LA)(P=0.043)were increased.The total cholesterol(TC)(P=0.008)and triglycerides(TG)(P=0.01)in serum were also reduced.Additionally,inulin addition elevated the relative abundance of several beneficial symbiotic and short-chain fatty acid(SCFA)-producing bacteria,such as Muribaculaceae(false discovery rate[FDR]-adjusted P<0.01),Acetitomaculum(FDR-adjusted P=0.043),and Bu tyrivibrio(FDR-adjusted P=0.036),while elevating the levels of L-lysine(FDR-adjusted P=4.24×10^(-3)),L-proline(FDR-adjusted P=0.0158),and L-phenylalanine(FDRadj usted P=0.027).In contrast,several pathogens and ruminal bacteria abu ndant in high-fat diets,such as Escherichia-Sh igella(FD R-adj usted P=0.022),Erysipelo trichaceaeUCG-004(FD R-adjusted P<0.01)and RF39(FDR-adjusted P=0.042)were decreased along with the reduction of lysophosphatidylcholine(LysoPC)(18:1(9 Z))(FDR-adjusted P=1.03×10^(-3)),LysoPC(16:0)(FDR-adjusted P=0.0108),LysoPC(18:2(9 Z,12 Z))(FDRadjusted P=1.65×10^(-3))and 8-methylnonenoate.In conclusion,dietary inulin supplementation could increase the relative abundance of commensal microbiota and SCFA-producing bacteria,upregulate amino acidmetabolism and downregulate lipid metabolism in the rumen of dairy cows,which might further improve lactation performance and the level of serum lipids. 关键词：INULIN Ruminal microbiota Metabolomics Milk quality Serum lipid Dairy cow

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Dietary supplementation with inulin improves lactation performance and serum lipids by regulating the rumen microbiome and metabolome in dairy cows

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