ZbAGL11, a class D MADS-box transcription factor of Zanthoxylum bungeanum, is involved in sporophytic apomixis

查看全文 作　　者：Xitong [1]Fei;Qianqian [1]Shi;Yichen [1]Qi;Shujie [1]Wang;Yu [1]Lei;Haichao [1]Hu;Yulin [1]Liu;Tuxi [1]Yang;Anzhi [1]Wei

机构地区：[1]College of Forestry,Northwest Agriculture and Forestry University,Yangling,Shaanxi,China 2 Research Centre for Engineering and Technology of Zanthoxylum State Forestry Administration,Yangling,Xianyang 712100,China

出　　处：《Horticulture Research》索引2021年第8卷第1期,共12页

基　　金：Nati onal Key Resea rch and Development Program project funding(2018YFD1000605)。 摘　　要：Apomixis is a reproductive model that bypasses sexual reproduction,so it does not require the combination of paternal and maternal gametes but instead results in the production of offspring directly from maternal tissues.This reproductive mode results in the same genetic material in the mother and the offspring and has signi fi cant applications in agricultural breeding.Molecular and cytological methods were used to identify the reproductive type of Zanthoxylum bungeanum(ZB).Fluorescence detection of the ampli fi ed products of 12 pairs of polymorphic SSR primers showed consistent fl uorescence signals for mother and offspring,indicating that no trait separation occurred during reproduction.In addition,the cytological observation results showed differentiation of ZB embryos(2n)from nucellar cells(2n)to form inde fi nite embryonic primordia and then form adventitious embryos(2n),indicating that the apomictic type of ZB is sporophytic apomixis.The MADS-box transcription factor ZbAGLll was highly expressed during the critical period of nucellar embryo development in ZB.Unpollinated ZbAGLll-OE Arabidopsis produced fertile offspring and exhibited an apomictic phenotype.The overexpression of ZbAGL11 increased the callus induction rate of ZB tissue.In addition,the results of the yeast two-hybrid experiment showed that ZbAGL11 could interact with the ZbCYP450 and ZbCAD11 proteins.Our results demonstrate that ZbAGL11 can cause developmental disorders of Arabidopsis fl ower organs and result in apomixis-like phenotypes. 关键词：MOTHER ARABIDOPSIS OFFSPRING

相关文献

参考文献（54）
引证文献（9）
耦合文献（33）

参考文献(54)

引证文献(9)

耦合文献(33)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

ZbAGL11, a class D MADS-box transcription factor of Zanthoxylum bungeanum, is involved in sporophytic apomixis

参考文献(54)

引证文献(9)

耦合文献(33)

用户提示