Overexpression of MdATG8i improves water use efficiency in transgenic apple by modulating photosynthesis, osmotic balance, and autophagic activity under moderate water deficit

查看全文 作　　者：Xin [1]Jia;Ke [1]Mao;Ping [1]Wang;Yu [1]Wang;Xumei [1]Jia;Liuqing [1]Huo;Xun [2]Sun;Runmin [1]Che;Xiaoqing [1]Gong;Fengwang [1]Ma

机构地区：[1]State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple,College of Horticulture,Northwest A&F University,712100 Yangling,Shaanxi,China;[2]Center of Pear Engineering Technology Research,State Key Laboratory of Crop Genetics and Germplasm Enhancement,College of Horticulture,Nanjing Agricultural University,210095 Nanjing,China

出　　处：《Horticulture Research》索引2021年第8卷第1期,共15页

基　　金：the National Key Research and Development Program of China(2019YFD1000102);the National Natural Science Foundation of China(31972391);the earmarked fund for the China Agricultural Research System(CARS-27)。 摘　　要：Water deficit is one of the major limiting factors for apple(Malus domestica)production on the Loess Plateau,a major apple cultivation area in China.The identification of genes related to the regulation of water use efficiency(WUE)is a crucial aspect of crop breeding programs.As a conserved degradation and recycling mechanism in eukaryotes,autophagy has been reported to participate in various stress responses.However,the relationship between autophagy and WUE regulation has not been explored.We have shown that a crucial autophagy protein in apple,MdATG8i,plays a role in improving salt tolerance.Here,we explored its biological function in response to long-term moderate drought stress.The results showed that MdATG8i-overexpressing(MdATG8i-OE)apple plants exhibited higher WUE than wild-type(WT)plants under long-term moderate drought conditions.Plant WUE can be increased by improving photosynthetic efficiency.Osmoregulation plays a critical role in plant stress resistance and adaptation.Under long-term drought conditions,the photosynthetic capacity and accumulation of sugar and amino acids were higher in MdATG8i-OE plants than in WT plants.The increased photosynthetic capacity in the OE plants could be attributed to their ability to maintain optimal stomatal aperture,organized chloroplasts,and strong antioxidant activity.MdATG8i overexpression also promoted autophagic activity,which was likely related to the changes described above.In summary,our results demonstrate that MdATG8i-OE apple lines exhibited higher WUE than WT under long-term moderate drought conditions because they maintained robust photosynthesis,effective osmotic adjustment processes,and strong autophagic activity. 关键词：breeding DROUGHT apple

相关文献

参考文献（81）
引证文献（4）
耦合文献（268）

参考文献(81)

引证文献(4)

耦合文献(268)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Overexpression of MdATG8i improves water use efficiency in transgenic apple by modulating photosynthesis, osmotic balance, and autophagic activity under moderate water deficit

参考文献(81)

引证文献(4)

耦合文献(268)

用户提示