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circ_0003204 regulates the osteogenic differentiation of human adipose-derived stem cells via miR-370-3p/HDAC4 axis

查看全文 作　　者：Liyuan [1]Yu;Kai [1]Xia;Jing [2]Zhou;Zhiai [1]Hu;Xing [1]Yin;Chenchen [3]Zhou;Shujuan [1]Zou;Jun [1]Liu 高影响力作者 机构地区：[1]State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases&Department of Orthodontics,West China Hospital of Stomatology,Sichuan University,Chengdu,China;[2]Department of Stomatology,Kunming Yan’an hospital,Kunming,China;[3]State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases&Department of Pediatric Dentistry,West China Hospital of Stomatology,Sichuan University,Chengdu,China高影响力机构 出　　处：《International Journal of Oral Science》索引2022年第14卷第3期,共11页高影响力期刊 基　　金：supported by grants from the National Natural Science Foundation of China(82071150,82170934,81870743,8190104 and 82171001)。 摘　　要：Human adipose-derived stem cells(hASCs)are a promising cell type for bone tissue regeneration.Circular RNAs(circRNAs)have been shown to play a critical role in regulating various cell differentiation and involve in mesenchymal stem cell osteogenesis.However,how circRNAs regulate hASCs in osteogenesis is still unclear.Herein,we found circ_0003204 was significantly downregulated during osteogenic differentiation of hASCs.Knockdown of circ_0003204 by si RNA or overexpression by lentivirus confirmed circ_0003204 could negatively regulate the osteogenic differentiation of hASCs.We performed dual-luciferase reporting assay and rescue experiments to verify circ_0003204 regulated osteogenic differentiation via sponging miR-370-3p.We predicted and confirmed that miR-370-3p had targets in the 3′-UTR of HDAC4 m RNA.The following rescue experiments indicated that circ_0003204 regulated the osteogenic differentiation of hASCs via miR-370-3p/HDAC4 axis.Subsequent in vivo experiments showed the silencing of circ_0003204 increased the bone formation and promoted the expression of osteogenic-related proteins in a mouse bone defect model,while overexpression of circ_0003204 inhibited bone defect repair.Our findings indicated that circ_0003204 might be a promising target to promote the efficacy of hASCs in repairing bone defects. 关 键 词：regulates the osteogenic differentiation of human adipose-derived stem cells via miR-370-3p/HDAC4 axis MIR
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