Disulfide-Containing Molecular Sticker Assists Cellular Delivery of DNA Nanoassemblies by Bypassing Endocytosis

查看全文 作　　者：Wen [1,2]Yang;Xiaochen [1]Liu;Haofei [3]Li;Jie [1]Zhou;Shan [1]Chen;Pengfei [3]Wang;Juan [1,2]Li;Huanghao [1]Yang

机构地区：[1]MOE Key Laboratory for Analytical Science of Food Safety and Biology,Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety,State Key Laboratory of Photocatalysis on Energy and Environment,College of Chemistry,Fuzhou University,Fuzhou 350108;[2]Institute of Cancer and Basic Medicine(IBMC),Chinese Academy of Sciences,Zhejiang 310022;[3]Institute of Molecular Medicine,Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine,State Key Laboratory of Oncogenes and Related Genes,Renji Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai 200240

出　　处：《CCS Chemistry》索引2021年第3卷第3期,共9页

基　　金：supported by the Natural Science Foundation of China(grant nos.91959102,21635002,and 61805041);the Natural Science Foundation of Fujian Province of China(grant no.2017J06004);the Shanghai Rising-Star Program(grant no.19QA1405400);the Program for Changjiang Scholars and Innovative Research Team in University(grant no.IRT15R11). 摘　　要：The delivery efficiency of DNA nanoassemblies to the cytosol remains unsatisfactory due to endoand lysosomal entrapment and degradation,which restricts their bioanalytical and biomedical applications.Herein,the authors developed a disulfide-containing molecular sticker(DSMS)assisted approach to achieve efficient cytosolic delivery of DNA nanoassemblies.DSMS is designed to consist of a disulfide unit for thiol-mediated uptake and a guanidinium cation unit for strongly adhering to DNA nanoassemblies.After attaching with DSMS,a set of DNA nanoassemblies maintained their original three-dimensional features but had a different cellular internalization pathway.These results demonstrated that DSMS-DNA nanoassemblies complex did not enter the cells via endocytosis but instead entered through thiol-mediated direct uptake.Taking advantages of this facile assembly,universal applicability,and direct cytosolic delivery,DSMS might serve as a potent agent to facilitate the applications of DNA nanoassemblies in a variety of fields,such as bioimaging,drug delivery,and gene therapy. 关键词：DNA nanoassemblies endocytosisindependent pathway lysosomal trapping thiolmediated uptake self-assembly

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Disulfide-Containing Molecular Sticker Assists Cellular Delivery of DNA Nanoassemblies by Bypassing Endocytosis

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