CHH methylation of genes associated with fatty acid and jasmonate biosynthesis contributes to cold tolerance in autotetraploids of Poncirus trifoliata

查看全文 作　　者：Yue [1]Wang;Lanlan [1]Zuo;Tonglu [1]Wei;Yu [1]Zhang;Yang [1]Zhang;Ruhong [1]Ming;Dahro [1]Bachar;Wei [1]Xiao;Khan [1]Madiha;Chuanwu [2]Chen;Qijun [2]Fan;Chunlong [1]Li;Ji-Hong [1]Liu

机构地区：[1]Key Laboratory of Horticultural Plant Biology(MOE),College of Horticulture and Foresty Sciences,Huazhong Agricultural University,Wuhan 430070,China;[2]Guangxi Key Laboratory of Citrus Biology,Guangxi Academy of Specialty Crops,Guilin 541004,China

出　　处：《Journal of Integrative Plant Biology》索引2022年第64卷第12期,共17页

基　　金：National Key Research and Development Program of China(2018YFD1000302);the National Natural Science Foundation of China(31972377)。 摘　　要：Polyploids have elevated stress tolerance,but the underlying mechanisms remain largely elusive.In this study,we showed that naturally occurring tetraploid plants of trifoliate orange(Poncirus trifoliata(L.) Raf.) exhibited enhanced cold tolerance relative to their diploid progenitors.Transcriptome analysis revealed that whole-genome duplication was associated with higher expression levels of a range of well-characterized cold stress-responsive genes.Global DNA methylation profiling demonstrated that the tetraploids underwent more extensive DNA demethylation in comparison with the diploids under cold stress.CHH methylation in the promoters was associated with up-regulation of related genes,whereas CG,CHG,and CHH methylation in the 3’-regions was relevant to gene down-regulation.Of note,genes involved in unsaturated fatty acids(UFAs) and jasmonate(JA)biosynthesis in the tetraploids displayed different CHH methylation in the gene flanking regions and were prominently up-regulated,consistent with greater accumulation of UFAs and JA when exposed to the cold stress.Collectively,our findings explored the difference in cold stress response between diploids and tetraploids at both transcriptional and epigenetic levels,and gained new insight into the molecular mechanisms underlying enhanced cold tolerance of the tetraploid.These results contribute to uncovering a novel regulatory role of DNA methylation in better cold tolerance of polyploids. 关键词：AUTOTETRAPLOID cold tolerance DNA methylation JA Poncirus trifoliata unsaturated fatty acid

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CHH methylation of genes associated with fatty acid and jasmonate biosynthesis contributes to cold tolerance in autotetraploids of Poncirus trifoliata

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