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Intravital imaging of adriamycin-induced renal pathology using two-photon microscopy and optical coherence tomography

查看全文 作　　者：Hengchang [1]Guo;Hsing-Wen [1]Wang;Qinggong [1]Tang;Erik [2]Anderson;Reuben [2]Falola;Tikina [3]Smith;Yi [1]Liu;Moshe [2]Levi;Peter [2]M.Andrews;Yu [1]Chen 高影响力作者 机构地区：[1]Fischell Department of Bioengineering University of Maryland College Park,MD 20742,USA;[2]Department of Biochemistry and Molecular&Cellular Biology Georgetown University Medical Center Washington DC 20007,USA;[3]Central Animal Resources Facility University of Maryland,College Park,MD 20742,USA高影响力机构 出　　处：《Journal of Innovative Optical Health Sciences》索引2018年第5期,共10页高影响力期刊 基　　金：the National Institutes of Health(NIH)Grant Nos.R21AG042700 and R21DK088066。 摘　　要：Adriamycin(doxorubicin),a common cancer chemotherapeutic drug,can be used to induce a model of chronic progressive glomerular disease in rodents.In our studies,we evahuated renal changes in a rat model after Adriamydin injection using two photon microscopy(TPM),optical coherence tomography(OCT)and Doppler OCT(DOCT).Taking advantage of deep penetra-tion and fast scanning speed for three dimensional(3D)label-free imaging,OCT/DOCT system was able to reveal glomerular and tubular pathology noninvasively and in real time.By imaging renal pathology following the infusion of fAuorophore-labeled dextrans of different molecular weights,TPM can provide direct views of glomerular and tubular flow dynamics with the onset and progression of renal disease.Specifically,glomerular permeability and filtration,proximal and distal tubular flow dynamics can be revealed.6-8 weeks after injection of Adriamycin,TPM and OCT/DOCT imaging revealed glomerular sclerosis,compromised flow across the glomerular wall,tubular atrophy,tubular dilation,and variable intra-tubular flow dynamics.Our results indicate that TPM and OCT/DOCT provide real-time imaging of renal pathology in vivo that has not been previously available using conventional microscopic procedures. 关 键 词：Chronic kidney disease(CKD) glomeruloscerosis Optical Coherence Tomography(OCT) Tubular Atrophy Two-photon Microscopy(TPM)
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