Establishment of a Rat Model of Liver Venous Deprivation:Simultaneous Portal and Hepatic Vein Ligation

查看全文 作　　者：Yuefeng [1]Zhang;Xiaoqin [2]He;Peng [1]Ma;Liangkun [1]Xiong;Wenhui [1]Bai;Gaoshuo [2]Zhang;Yangtao [3]Xu;Wei [3]Song;Kaihuan [1]Yu 机构地区：[1]Department of Hepatobiliary Surgery,Renmin Hospital of Wuhan University,Wuhan,Hubei,China;[2]Department of Teaching Office,Renmin Hospital of Wuhan University,Wuhan,Hubei,China;[3]The First Clinical College,Wuhan University,Wuhan,Hubei,China 出　　处：《Journal of Clinical and Translational Hepatology》索引2023年第11卷第2期,共12页 基　　金：supported by the Fundamental Research Funds for the Central Universities (NO.2042020kf0124);the National Natural Science Foundation of China (NO.82001940). 摘　　要：Background and Aims:The aim was to establish a liver venous deprivation(LVD)model in rats,compare hepatic hypertrophy between LVD and associated liver partition and portal vein ligation for staged hepatectomy(ALPPS),and explore the underlying mechanisms.Methods:The LVD or extended-LVD(e-LVD)group received portal vein ligation(PVL)combined with hepatic vein ligation(HVL).The ALPPS or eALPPS group received PVL plus parenchyma ligation.Liver regeneration was assessed by measuring the liver weight and performing pathological analysis.Liver functions and the sphingosine kinase 1(SPHK1)/sphingosine-1-phosphate(S1P)/sphingosine-1-phosphate receptor 1(S1PR1)pathway were also investigated.Results:All future liver remnants(FLRs)in the ALPPS,e-ALPPS,LVD,and e-LVD groups exhibited significant hypertrophy compared with the control group.The LVD and e-LVD procedures induced similar liver hypertrophy than that in the corresponding ALPPS groups.Furthermore,the LVD and e-LVD methods led to obvious cytolysis in the venous-deprived lobes as well as a noticeable increase in serum transaminase levels,while no necrosis was observed in the ALPPS and e-ALPPS groups.SPHK1/S1P/S1PR1 pathway were distinctly activated after operation,especially in congestive/ischemic livers.Conclusions:We describe the first rat model of LVD and e-LVD with simultaneously associated HVL and PVL.Compared with the ALPPS technique,the LVD or e-LVD procedure had a comparable overall effect on the hypertrophy response and a stronger effect on liver function.The SPHK1/S1P/S1PR1 pathway was involved in the LVD-or ALPPS-induced liver remodeling. 关 键 词：HEPATECTOMY Portal vein Hepatic vein HYPERTROPHY Animal model