Protective effect of resveratrol against cadmium-induced toxicity on ovine oocyte in vitro maturation and fertilization

查看全文 作　　者：Anna Rita [1]Piras;Federica [1]Ariu;Alessio [1]Maltana;Giovanni Giuseppe [1]Leoni;Nicola Antonio [2]Martino;Antonella [2]Mastrorocco;Maria Elena Dell’[2]Aquila;Luisa [1]Bogliolo

机构地区：[1]Department of Veterinary Medicine,University of Sassari,07100 Sassari,Sardinia,Italy.;[2]Department of Biosciences,Biotechnologies&Biopharmaceutics,University of Bari Aldo Moro,70125 Bari,Italy.

出　　处：《Journal of Animal Science and Biotechnology》索引2023年第14卷第1期,共14页

基　　金：funded by Fondazione Banco di Sardegna,FDS 2016(CUP J86C18000780005 and J86C18000810005)。 摘　　要：Background:Heavy metal cadmium(Cd)is a widespread environmental contaminant with a potential toxicity that might negatively affect female reproduction and fertility.It has been reported that Cd exposure impaired the quality of oocytes and led to a defective maturation and fertilization,through oxidative stress induction.Resveratrol(Res)is a natural polyphenol with strong antioxidant properties that exhibited protective role in preventing oocyte redox homeostasis disruption and quality decline.Here,we explored whether the addition of Res to in vitro maturation(IVM)medium might act as a protection against Cd-induced toxicity on ovine oocyte maturation and fertilization.Firstly,we evaluated the effect of supplementing IVM medium with two different Res concentrations(1and 2μmol/L)on nuclear maturation and fertilization of oocytes matured under CdCl2(2μmol/L)exposure.Therefore,the concentration of 1μmol/L Res was selected to analyse the effects of this compound on intracellular ROS levels,mitochondrial(mt)distribution and activity,chromatin configuration,cytoskeleton morphology,cortical granules(CGs)distribution and mRNA expression of genes associated with cellular response to oxidative stress(i.e.SIRT1,SOD 1,GPX1,GSR,CAT)in Cd-exposed in vitro matured oocytes.Results:We found that 1μmol/L Res restored the reduced oocyte meiotic competence induced by Cd exposure as well as,Res sustained oocyte ability to be normally fertilized and decreased polyspermic fertilization at both tested concentrations.Moreover,we demonstrated that 1μmol/L Res mitigated Cd-induced alterations of oocyte cytoplasmic maturation by reducing reactive oxygen species(ROS)accumulation,preventing mt dysfunction,maintaining the correct meiotic spindle and cortical F-actin assembly and the normal cortical granule distribution as well as up-regulating SIRT1,SOD1 and GPX1 genes.Conclusions:Taken together,our findings highlighted the beneficial influence exerted by Res in preventing Cdinduced disturbance of nuclear and cytoplasmic maturation and subsequent fertilization in ovine oocytes.Res treatment may help to establish defence strategies counteracting Cd-induced toxicity on the female gamete. 关键词：Cadmium In vitro maturation OOCYTE OVINE RESVERATROL

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Protective effect of resveratrol against cadmium-induced toxicity on ovine oocyte in vitro maturation and fertilization

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