The roles of phosphate in shaping the structure and dynamics of Antarctic soil microbiomes

查看全文 作　　者：TAN [1]Jiankang;CAO [2]Huansheng;LIU [2]Li;QIN [3]Yiling;LIU [3]Feng;John [4]CAVA;YIN [3]Xiaofei;SHEN [3]Jihong;WANG [5]Nengfei 机构地区：[1]Department of Ecology and Environment of Lishui,Nanjing 200200,China;[2]Division of Natural and Applied Sciences,Duke Kunshan University,Suzhou 215316,China;[3]The First Institute of Oceanography,Ministry of Natural Resources,Qingdao 266061,China;[4]Center for Fundamental and Applied Microbiomics,Biodesign Institute,Arizona State University,Tempe,AZ 85287,USA;[5]School of Chemistry and Chemical Engineering,Linyi University,Linyi 276000,China 出　　处：《Advances in Polar Science》索引2023年第34卷第1期,共17页 基　　金：funded by the National Key R&D Program of China(Grant no.2018YFC1406700);the National Natural Science Foundation of China(Grant no.41776198);the Basic Scientific Fund for National Public Research Institutes of China(Grant no.GY0219Q10)。 摘　　要：One major consequence of global warming in the Antarctic region is increased ice-free zones.Subsequent colonization of these ice-free areas by penguins alters their biogeochemistry,with one prominent example being elevation of inorganic phosphate concentrations around feces depositions.The complex soil biochemistry in the region makes it difficult to define the causal factors of these changes using common research approaches.Here,we addressed the effects of phosphate alone on microbiome structure and dynamics over time by adding external phosphate to selected soils in the Antarctic region.We then analyzed the soil bacterial community composition and diversity using 16S rRNA amplicon sequencing and compared these data with phosphate levels.Parallel geochemical analysis revealed changes in nine soil geochemical factors upon phosphate addition,all of which were relevant to microbiome structure,with soil pH showing the highest correlation.Links between geochemical factors and composition were identified,as were interactions between bacterial taxa.Additionally,Sphingobacteriia,Sphingobacteriales and Chitinophagaceae were found to be more abundant in phosphate-treated soils.Co-occurrence network analysis revealed significantly increased levels of associations in all major network properties over time after phosphate supplementation.Therefore,we conclude phosphate addition has diverse effects on Antarctic soil microbiomes. 关 键 词：PHOSPHATE ANTARCTIC microbiome composition network geochemical factors ice-free zone