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Click and bioorthogonal hyaluronic acid hydrogels as an ultra-tunable platform for the investigation of cell-material interactions

查看全文 作　　者：Nathan [1]Lagneau;Pierre [1]Tournier;Boris [1]Halgand;François [1]Loll;Yves [1]Maugars;Jérôme [1]Guicheux;Catherine Le [1]Visage;Vianney [1]Delplace 高影响力作者 机构地区：[1]Nantes Université,Oniris,CHU Nantes,INSERM,Regenerative Medicine and Skeleton,RMeS,UMR 1229,F-44000,France高影响力机构 出　　处：《Bioactive Materials》索引2023年第6期,共12页高影响力期刊 基　　金：the Fondation de l’Avenir pour la Recherche Médicale Appliquée(AP-RM-18-005,CLV);the Fondation pour la Recherche M´edicale(ARF201809007012,VD);the Nantes Excellence Trajectory program(NExT Junior Talent 2018,VD,NexT IIP Shelby 2018,CLV);the Marie-Sklodowska Curie Actions(BABHY-CART project,GAP-846477,VD)for their financial support. 摘　　要：The cellular microenvironment plays a major role in the biological functions of cells.Thus,biomaterials,especially hydrogels,which can be design to mimic the cellular microenvironment,are being increasingly used for cell encapsulation,delivery,and 3D culture,with the hope of controlling cell functions.Yet,much remains to be understood about the effects of cell-material interactions,and advanced synthetic strategies need to be developed to independently control the mechanical and biochemical properties of hydrogels.To address this challenge,we designed a new hyaluronic acid(HA)-based hydrogel platform using a click and bioorthogonal strain-promoted azide-alkyne cycloaddition(SPAAC)reaction.This approach facilitates the synthesis of hydrogels that are easy to synthesize and sterilize,have minimal swelling,are stable long term,and are cytocompatible.It provides bioorthogonal HA gels over an uncommonly large range of stiffness(0.5-45 kPa),all forming within 1-15 min.More importantly,our approach offers a versatile one-pot procedure to independently tune the hydrogel composition(e.g.,polymer and adhesive peptides).Using this platform,we investigate the independent effects of polymer type,stiffness,and adhesion on the secretory properties of human adipose-derived stromal cells(hASCs)and demonstrate that HA can enhance the secretion of immunomodulatory factors by hASCs. 关 键 词：HYDROGELS Click and bioorthogonal chemistry Hyaluronic acid Mesenchymal stromal cells Cell-material interactions SECRETOME
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