Supramolecular assembly of Cp1-11 peptide and insulin for rapid-acting formulation

查看全文 作　　者：Weigang [1]Wang;Sheyu [3]Li;Zhouxiang [1]Zhao;Anna [1]Zhou;Yanpeng [1]Liu;Yantao [2]Chen;Mingchang [4]Lin;Guosong [4]Chen;Chunmei [1]Ding;Jianshu [1]Li 机构地区：[1]College of Polymer Science and Engineering,State Key Laboratory of Polymer Materials Engineering,Sichuan University,Chengdu 610065,China;[2]Shenzhen Key Laboratory of Functional Polymer,College of Chemistry and Chemical Engineering,Shenzhen University,Shenzhen 518060,China;[3]Department of Endocrinology and Metabolism,West China Hospital,Sichuan University,Chengdu 610041,China;[4]State Key Laboratory of Molecular Engineering of Polymers and Department of Macromolecular Science,Fudan University,Shanghai 200433,China 出　　处：《Journal of Bioresources and Bioproducts》索引2017年第2卷第3期,共10页 基　　金：supported by National Natural Science Foundation of China (21534008,51322303 and 21174088);Program for Changjiang Scholars and Innovative Research Team in University (IRT_15R48);State Key Laboratory of Polymer Materials Engineering (Grant No.sklpme2017-2-02). 摘　　要：In order to improve the life quality of diabetic patients,it is very important to develop rapid-acting insulin formulations that can mimic the physiological meal-time secretion profile of insulin in healthy people.Although several insulin analogues have been designed to provide postprandial glycemic control,still there are some serious disadvantages.A supramolecular strategy is presented here to inhibit insulin aggregation and improve its bioactivity by using Cp1-11 peptide.As a fragment of C-peptide in proinsulin,Cp1-11 peptide was found to influence insulin oligomerization by supramolecular interactions.This work demonstrates that the Cp1-11 peptide can interact with oligomeric insulin and facilitate its disaggregation into the physiologically active monomeric form.Computer simulation indicates that Cp1-11 can insert into the space between the C-terminal tail and the N-terminal helix of the B-chain of insulin,causing dissociation of the insulin dimer.The supramolecular assembly of Cp1-11 and insulin can improve the bioavailability and therapeutic effect of insulin on the control of in vivo blood glucose levels.These results suggest that Cp1-11 peptide can modulate the intermolecular interaction of aggregated insulin and prevent the transition from monomeric to multimeric states,and shows great potential for the development of an effective rapid-acting strategy to treat diabetes. 关 键 词：Supramolecular chemistry Cp1-11 peptide Drug delivery SELF-ASSEMBLY Rapid-acting insulin