Shen Qi Wan attenuates renal interstitial fibrosis through upregulating AQP1

查看全文 作　　者：LIN [1]Yiyou;WEI [1]Jiale;ZHANG [1]Yehui;HUANG [1]Junhao;WANG [1]Sichen;LUO [1]Qihan;YU [1]Hongxia;JI [1]Liting;ZHOU [2]Xiaojie;LI [1,2]Changyu

机构地区：[1]School of Pharmaceutical Sciences,Zhejiang Chinese Medical University,Hangzhou 310053,China;[2]Academy of Chinese Medical Sciences,Zhejiang Chinese Medical University,Hangzhou 310053,China

出　　处：《Chinese Journal of Natural Medicines》索引2023年第21卷第5期,共12页

基　　金：the National Natural Science Foundation of China(Nos.81673839 and 82074304);the Young Talents Foundation of Zhejiang Traditional Chinese Medicine Science and Technology Program(No.2019ZQ014);the Science Foundation of Zhejiang Chinese Medical University(No.2020ZZ13). 摘　　要：Renal interstitial fibrosis(RIF)is the crucial pathway in chronic kidney disease(CKD)leading to the end-stage renal failure.However,the underlying mechanism of Shen Qi Wan(SQW)on RIF is not fully understood.In the current study,we investigated the role of Aquaporin 1(AQP1)in SQW on tubular epithelial-to-mesenchymal transition(EMT).A RIF mouse model induced by adenine and a TGF-β1-stimulated HK-2 cell model were etablished to explore the involvement of AQP 1 in the protective effect of SQW on EMT in vitro and in vivo.Subsequently,the molecular mechanism of SQW on EMT was explored in HK-2 cells with AQP1 knockdown.The results indicated that SQW alleviated kidney injury and renal collagen deposition in the kidneys of mice induced by adenine,increased the protein expression of E-cadherin and AQP1 expression,and decreased the expression of vimentin andα-smooth muscle actin(α-SMA).Similarly,treatmement with SQW-containing serum significantly halted EMT process in TGF-β1 stimulated HK-2 cells.The expression of snail and slug was significantly upregulated in HK-2 cells after knockdown of AQP1.AQP1 knockdown also increased the mRNA expression of vimentin andα-SMA,and decreased the expression of E-cadherin.The protein expression of vimentin increased,while the expression of E-cadherin and CK-18 significantly decreased after AQP1 knockdown in HK-2 cells.These results revealed that AQP1 knockdown promoted EMT.Furthermore,AQP1 knockdown abolished the protective effect of SQW-containing serum on EMT in HK-2 cells.In sum,SQW attentuates EMT process in RIF through upregulation of the expression of AQP1. 关键词：Shen Qi Wan Chronic kidney disease Renal interstitial fibrosis Epithelial to mesenchymal transition Aquaporin 1

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Shen Qi Wan attenuates renal interstitial fibrosis through upregulating AQP1

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