Enzyme@bismuth-ellagic acid:a versatile platform for enzyme immobilization with enhanced acid-base stability

查看全文 作　　者：Junyang [1]Xu;Guanhua [1,2]Liu;Ying [1]He;Liya [1]Zhou;Li [1]Ma;Yunting [1]Liu;Xiaobing [1,2]Zheng;Jing [1]Gao;Yanjun [1,2]Jiang 机构地区：[1]School of Chemical Engineering and Technology,Hebei University of Technology,Tianjin 300130,China;[2]National-Local Joint Engineering Laboratory for Energy Conservation of Chemical Process Integration and Resources Utilization,Hebei University of Technology,Tianjin 300130,China 出　　处：《Frontiers of Chemical Science and Engineering》索引2023年第17卷第6期,共11页 基　　金：financially supported by the National Natural Science Foundation of China(Grant Nos.22178083,22078081 and 21878068);the Natural Science Foundation of Tianjin China(Grant No.20JCYBJC00530);the Hebei Key Research and Development Project(Grant No.20372802D);Open Funding Project of the State Key Laboratory of Biocatalysis and Enzyme Engineering(Grant No.SKLBEE2020011);Science Technology Research Project of Higher Education of Hebei Province(Grant No.QN2021045);Tianjin Enterprise Science and Technology Commissioner Project(Grant No.21YDTPJC00810). 摘　　要：In situ encapsulation is an effective way to synthesize enzyme@metal–organic framework biocatalysts;however,it is limited by the conditions of metal–organic framework synthesis and its acid-base stability.Herein,a biocatalytic platform with improved acid-base stability was constructed via a one-pot method using bismuth-ellagic acid as the carrier.Bismuth-ellagic acid is a green phenol-based metal–organic framework whose organic precursor is extracted from natural plants.After encapsulation,the stability,especially the acid-base stability,of amyloglucosidases@bismuth-ellagic acid was enhanced,which remained stable over a wide pH range(2–12)and achieved multiple recycling.By selecting a suitable buffer,bismuth-ellagic acid can encapsulate different types of enzymes and enable interactions between the encapsulated enzymes and cofactors,as well as between multiple enzymes.The green precursor,simple and convenient preparation process provided a versatile strategy for enzymes encapsulation. 关 键 词：bismuth-ellagic acid in situ encapsulation enzyme@MOF biocomposites