维普中文期刊产品整合服务

Sample Collection,DNA Extraction,and Library Construction Protocols of the Human Microbiome Studies in the International Human Phenome Project

查看全文 作　　者：Yetong [1]Wang;Ruyi [3]Zhang;Yanni [1]Pu;Danqi [3]Wang;Yanren [3]Wang;Xuemei [1]Wu;Yujie [1]Pan;Chen [1]Luo;Guoping [1,4]Zhao;Zhexue [3]Quan;Yan [1,2]Zheng 高影响力作者 机构地区：[1]State Key Laboratory of Genetic Engineering,Human Phenome Institute,and School of Life Sciences,Fudan University,2005 Songhu Road,Shanghai 200433,China;[2]Department of Cardiology,Shanghai Institute of Cardiovascular Disease,Zhongshan Hospital,Fudan University,1609 Xietu Road,Shanghai 200032,China;[3]Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering,Institute of Biodiversity Science,School of Life Sciences,Fudan University,2005 Songhu Road,Shanghai 200433,China;[4]Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200032,China高影响力机构 出　　处：《Phenomics》索引2023年第3卷第3期,共9页高影响力期刊 基　　金：supported by the National Key Research and Development Program of China(2021YFA1301000);the Shanghai Municipal Science and Technology Major Project(2017SHZDZX01). 摘　　要：The human microbiome plays a crucial role in human health.In the past decade,advances in high-throughput sequencing technologies and analytical software have significantly improved our knowledge of the human microbiome.However,most studies concerning the human microbiome did not provide repeatable protocols to guide the sample collection,handling,and processing procedures,which impedes obtaining valid and timely microbial taxonomic and functional results.This protocol provides detailed operation methods of human microbial sample collection,DNA extraction,and library construction for both the amplicon sequencing-based measurements of the microbial samples from the human nasal cavity,oral cavity,and skin,as well as the shotgun metagenomic sequencing-based measurements of the human stool samples among adult par-ticipants.This study intends to develop practical procedure standards to improve the reproducibility of microbiota profiling of human samples. 关 键 词：Sample collection DNA extraction Library construction Human microbiome
相关文献

参考文献(31)

引证文献(1)

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1　渝公网安备 50019002500403号　违法和不良信息举报中心

互联网出版许可证 新出网证(渝)字10号 全国400电话 - 免长途话费