Determination of Total Flavonoids in Leaves of Tetracera asiatica

查看全文 作　　者：Fangfang [1,2]DA;Caoju [1]MO;Wenxiu [3]HUANG;Yulin [4]LU;Qiying [1]YANG;Qing [1]ZHANG 机构地区：[1]School of Chinese Ethnic Medicine,Guizhou Minzu University,Guiyang 550025,China;[2]Key Laboratory of the Development and Utilization of Guizhou Minority Medical Resources(Guizhou Minzu University),State Ethnic Affairs Commission,Guiyang 550025,China;[3]School of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530001,China;[4]Bio-Thera Solutions,Ltd.,Guangzhou 510700,China 出　　处：《Medicinal Plant》索引2023年第14卷第4期,共4页 基　　金：Supported by Regional Fund of the National Natural Science Foundation of China(82160820);General Project of Natural Science Foundation of Guizhou Province(QKHJC-ZK[2023]YB 153);Startup Foundation for Introduced Doctors in 2018(2018BS047). 摘　　要：[Objectives] This study was conducted to determine and compare the contents of flavonoids in the leaves of Tetracera asiatica , an ethnic medicine from different habitats, in order to provide a basis for the quality control of its medicinal materials. [Methods] The flavonoid compounds in the leaves of T. asiatica were reflux-extracted in a water bath, and the total flavonoid content in T. asiatica was determined by ultraviolet-visible spectrophotometry. [Results] The total flavonoid content in T. asiatica leaves varied according to different habitats. The highest content was found in Gaofeng (19.41%) of Guangxi, followed by Huizhou (16.88%) of Guangdong and Hong Kong (16.76%). The lowest total flavonoid content was found in Shangcuntang (3.91%) of Bobai County, Guangxi, followed by Beiliu (4.15%), Guangxi. The total flavonoid contents in the samples ranged from 3.91% to 19.41%, with an average content of about 12.36%. [Conclusions] The UV spectrophotometry method is easy to operate, and has good repeatability, accurate detection results, and high reliability, and can be used for the determination of flavonoids in T. asiatica . 关 键 词：Leaves of Tetracera asiatica Total flavonoids Content determination HABITAT