Genetic Differentiation and Conservation of 14 Surviving Individuals of Euryodendron excelsum Endemic to China

查看全文 作　　者：Wang [1,2]Ting;Su [1]Yingjuan;Ye [3]Huagu;Ouyang [1]Puyue;Jiang [1]Yu;Sun [1]Yufei;Chen [1]Guopei;Deng [1]Feng;Zhang [1]Hongda 机构地区：[1]School of Life Sciences,Sun Yat-sen University,Guangzhou 510275,China;[2]Wuhan Botanical Garden,Chinese Academy of Sciences,Wuhan 430074,China;[3]South China Botanical Garden,Chinese Academy of Sciences,Guangzhou 510650,China 出　　处：《Frontiers in Biology》索引2006年第1卷第1期,共6页 基　　金：supported by National Natural Science Foundation of China (No.30271094);the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry (No.[2003]406);the“100 Talent Project”,the Chinese Academy of Sciences (No.05052903). 摘　　要：Random amplification polymorphicDNA(RAPD)markers were used to assess the genetic variations and the evolutionary relationships among all 14 individuals of a critically endangered Euryodendron excelsum(Theaceae)population distributed in Ba Jia Zhen,Yangchun,Guangdong,China.Twenty-three random primers detected 156 sites,out of which 95(60.26%)were polymorphic loci.The number of the observed alleles was 1.6090,and the number of the effective alleles was 1.3471.Nei’s gene diversity was 0.1993,and Shannon index was 0.1534.A relatively high level of genetic variation was identified in E.excelsum.An unweighted pair group method with arithmetic mean(UPGMA)tree established from Jaccard similarity coefficients suggested that 14 individuals were clustered into two subgroups and that the No.2 plant was genetically distant from the rest of the individuals.The UPGMA clustering was also supported by a principle components analysis of RAPD phenotypic data.The management and conservation strategy of E.excelsum was proposed based on our results. 关 键 词：Euryodendron excelsum population genetic variation RAPD markers conservation management