Establishment of induced pluripotent stem cells from aged mice using bone marrow-derived myeloid cells

查看全文 作　　者：Zhao [1]Cheng;Sachiko [1]Ito;Naomi [1]Nishio;Hengyi [2]Xiao;Rong [1]Zhang;Haruhiko [1]Suzuki;Yayoi [3]Okawa;Toyoaki [4]Murohara;Ken-ichi [1]Isobe

机构地区：[1]Department of Immunology,Nagoya University Graduate School of Medicine,65 Turumai-cho,Showa-ku,Nagoya,Aichi 466-8520,Japan;[2]Laboratory for Geriatrics,State Key Laboratory of Biotherapy,Sichuan University,Chengdu 610041,China;[3]Department of Functioning Activation,National Institute for Longevity Sciences,National Center for Geriatrics Gerontology,Aichi 463-0003,Japan;[4]Department of Cardiology,Nagoya University Graduate School of Medicine,65 Turumai-cho,Showa-ku,Nagoya,Aichi 466-8520,Japan

出　　处：《Journal of Molecular Cell Biology》索引2011年第3卷第2期,共8页

基　　金：This work was supported by the Ministry of Education,Science,Technology,Sports and Culture,Japan。 摘　　要：If induced pluripotent stem(iPS)cells are to be used to treat damaged tissues or repair organs in elderly patients,it will be necessary to establish iPS cells from their tissues.To determine the feasibility of using this technology with elderly patients,we asked if it was indeed possible to establish iPS cells from the bone marrow(BM)of aged mice.BM cells from aged C57BL/6 mice carrying the green fluorescence protein(GFP)gene were cultured with granulocyte macrophage-colony stimulating factor(GM-CSF)for 4 days.Four factors(Oct3/4,Sox2,Klf4 and c-Myc)were introduced into the BM-derived myeloid(BM-M)cells.The efficiency of generating iPS cells from aged BM cultured in GM-CSF was low.However,we succeeded in obtaining BM-M-iPS cells from aged C57BL/6 mice,which carried GFP.Our BM-M-iPS cells expressed SSEA-1 and Pou5f1 and were positive for alkaline phosphatase staining.The iPS cells did make teratoma with three germ layers following injection into syngeneic C57BL/6 mice,and can be differentiated to three germ layers in vitro.By co-culturing with OP9,the BM-M-iPS cells can be differentiated to the myeloid lineage.The differentiated BM-M-iPS cells proliferated well in the presence of GM-CSF,and lost expression of Nanog and Pou5f1,at least in part,due to methylation of their promoters.On the contrary,Tnf and Il1b gene expression was upregulated and their promoters were hypomethylated. 关键词：induced pluripotent stem cells aged mouse bone marrow myeloid cells gene expression METHYLATION ChIP assay

相关文献

参考文献（42）
引证文献（1）
耦合文献（0）

参考文献(42)

引证文献(1)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Establishment of induced pluripotent stem cells from aged mice using bone marrow-derived myeloid cells

参考文献(42)

引证文献(1)

用户提示