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Magnetorheological elastomer and smartphone enable microfluidic biosensing of foodborne pathogen

查看全文 作　　者：Gaozhe [1,2]Cai;Yuhe [1]Wang;Yingchao [1]Zhang;Lingyan [3]Zheng;Jianhan [1]Lin 高影响力作者 机构地区：[1]Key Laboratory of Agricultural Information Acquisition Technology,Ministry of Agriculture and Rural Affairs,China Agricultural University,Beijing 100083,China;[2]State Key Laboratory of Transducer Technology,Shanghai Institute of Microsystem and Information Technology,Chinese Academy of Sciences,Shanghai 200050,China;[3]Beijing Engineering and Technology Research Center of Food Additives,Beijing Technology&Business University(BTBU),Beijing 100048,China高影响力机构 出　　处：《Chinese Chemical Letters》索引2023年第34卷第8期,共5页高影响力期刊 基　　金：supported by Walmart Foundation(No.UA2020-154). 摘　　要：Rapid detection of foodborne pathogens is crucial to prevent the outbreaks of foodborne diseases.In this work,we proposed a novel microfluidic biosensor based on magnetorheological elastomer(MRE)and smartphone.First,micropump and microvalves were constructed by deforming the MRE under magnetic actuation and integrated into the microfluidic biosensor for fluidic control.Then,the micropump was used to deliver immune porous gold@platinum nanocatalysts(Au@PtNCs),bacterial sample,and immunomagnetic nanoparticles(MNPs)into a micromixer,where they were mixed,incubated and magnetically separated to obtain the Au@PtNC-bacteria-MNP complexes.After 3,3',5,5'-tetramethylbenzidine and hydrogen peroxide were injected and catalyzed by the Au@PtNCs,smartphone was used to measure the color of the catalysate for quantitative analysis of target bacteria.Under optimal conditions,this biosensor could detect Salmonella typhimurium quantitatively and automatically in 1 h with a linear detection range of 8.0×10^(1) CFU/mL to 8.0×10^(4) CFU/mL and a detection limit of 62 CFU/mL.The microfluidic biosensor was compact in size,simple to use,and efficient for detection,and might be used for in-field screening of foodborne pathogens to prevent food poisoning. 关 键 词：Magnetorheological elastomer Microfluidic chip Colorimetric biosensor Bacterial detection Smartphone image processing
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