维普中文期刊产品整合服务

Improved in situ sequencing for high-resolution targeted spatial transcriptomic analysis in tissue sections

查看全文 作　　者：Xinbin [1]Tang;Jiayu [1]Chen;Xinya [1]Zhang;Xuzhu [1]Liu;Zhaoxiang [1]Xie;Kaipeng [2]Wei;Jianlong [2]Qiu;Weiyan [1]Ma;Chen [1]Lin;Rongqin [1]Ke 高影响力作者 机构地区：[1]School of Medicine and School of Biomedical Sciences,Huaqiao University,Quanzhou,Fujian 362021,China;[2]Department of Pathology,The 910 Hospital,Quanzhou,Fujian 362000,China高影响力机构 出　　处：《Journal of Genetics and Genomics》索引2023年第50卷第9期,共9页高影响力期刊 基　　金：supported by the Natural Science Foundation of Fujian Province(2022J06022);the Quanzhou Science and Technology Plan Project(2021C040R);the Scientific Research Funds of Huaqiao University. 摘　　要：Spatial transcriptomics enables the study of localization-indexed gene expression activity in tissues,providing the transcriptional landscape that in turn indicates the potential regulatory networks of gene expression.In situ sequencing(ISS)is a targeted spatial transcriptomic technique,based on padlock probe and rolling circle amplification combined with next-generation sequencing chemistry,for highly multiplexed in situ gene expression profiling.Here,we present improved in situ sequencing(IISS)that exploits a new probing and barcoding approach,combined with advanced image analysis pipelines for high-resolution targeted spatial gene expression profiling.We develop an improved combinatorial probe anchor ligation chemistry using a 2-base encoding strategy for barcode interrogation.The new encoding strategy results in higher signal intensity as well as improved specificity for in situ sequencing,while maintaining a streamlined analysis pipeline for targeted spatial transcriptomics.We show that IISS can be applied to both fresh frozen tissue and formalin-fixed paraffin-embedded tissue sections for single-cell level spatial gene expression analysis,based on which the developmental trajectory and cell-cell communication networks can also be constructed. 关 键 词：Spatial transcriptomics In situsequencing Single cell Geneexpression profiling Imaging
相关文献

参考文献(33)

引证文献(5)

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1　渝公网安备 50019002500403号　违法和不良信息举报中心

互联网出版许可证 新出网证(渝)字10号 全国400电话 - 免长途话费