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Pijx confers broad-spectrum seedling and panicle blast resistance by promoting the degradation of ATP β subunit and OsRbohC-mediated ROS burst in rice

查看全文 作　　者：Ning [1]Xiao;Yunyu [1]Wu;Xiaoxiang [1]Zhang;Zeyun [2]Hao;Zichun [1]Chen;Zefeng [3]Yang;Yue [1]Cai;Ruyi [2]Wang;Ling [1]Yu;Zhiping [1]Wang;Yue [3]Lu;Wei [1]Shi;Cunhong [1]Pan;Yuhong [1]Li;Changhai [1]Zhou;Jianju [1]Liu;Niansheng [1]Huang;Guangqing [1]Liu;Hongjuan [1]Ji;Shuhao [1]Zhu;Shuai [1]Fang;Yuese [2]Ning;Aihong [1]Li 高影响力作者 机构地区：[1]Institute of Agricultural Sciences for Lixiahe Region in Jiangsu,Yangzhou Rice Experiment Station of the China Agricultural Research System,Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding,Yangzhou 225009,China;[2]State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China;[3]Key Laboratory of Plant Functional Genomics,Ministry of Education,Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding,Agricultural College of Yangzhou University,Yangzhou 225009,China高影响力机构 出　　处：《Molecular Plant》索引2023年第16卷第11期,共15页高影响力期刊 基　　金：supported by the National Natural Science Foundation of China(grants No.31971868,32161143009,and 31971873);the earmarked fund for CARS-01-88CARS-01-88,the Core Provenance Project in Jiangsu Province(JBGS(2021)035);the Key R&D Program of Jiangsu Province Modern Agriculture(BE2021334,BE2022336);the Ministry of Science and Technology/State Administration of Foreign Experts Affairs(GL20200114001);the Jiangsu Province Natural Science Fund(BK20201218). 摘　　要：Rice blast,caused by the fungal pathogen Magnaporthe oryzae,is one of the most important diseases of rice.Utilization of blast-resistance genes is the most economical,effective,and environmentally friendly way to control the disease.However,genetic resources with broad-spectrum resistance(BSR)that is effective throughout the rice growth period are rare.In this work,using a genome-wide association study,we identify a new blast-resistance gene,Pijx,which encodes a typical CC-NBS-LRR protein.Pijx is derived from a wild rice species and confers BSR to M.oryzae at both the seedling and panicle stages.The functions of the resistant haplotypes of Pijx are confirmed by gene knockout and overexpression experiments.Mechanistically,the LRR domain in Pijx interacts with and promotes the degradation of the ATP synthase β subunit(ATPb)via the 26S proteasome pathway.ATPb acts as a negative regulator of Pijx-mediated panicle blast resistance,and interacts with OsRbohC to promote its degradation.Consistently,loss of ATPb function causes an increase in NAPDH content and ROS burst.Remarkably,when Pijx is introgressed into two japonica rice varieties,the introgression lines show BSR and increased yields that are approximately 51.59%and 79.31%higher compared with those of their parents in a natural blast disease nursery.In addition,we generate PPL^(Pijx Pigm) and PPL^(Pijx Piz-t) pyramided lines and these lines also have higher BSR to panicle blast compared with Pigm-or Piz-t-containing rice plants.Collectively,this study demonstrates that Pijx not only confers BSR to M.oryzae but also maintains high and stable rice yield,providing new genetic resources and molecular targets for breeding rice varieties with broad-spectrum blast resistance. 关 键 词：panicle blast broad-spectrum resistance Pijx ATPB ROS burst
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