A dual enzyme-phosphate hybrid nanoflower for glutamate detection

查看全文 作　　者：Peikun [1]Li;Jiahui [1]Jia;Zixin [1]Geng;Saizhao [1]Pang;Ruirui [1]Wang;Muhammad [3]Bilal;Hongjie [2]Bian;Jiandong [1]Cui;Shiru [1]Jia 机构地区：[1]State Key Laboratory of Food Nutrition and Safety,Laboratory of Industrial Fermentation Microbiology,Ministry of Education,Tianjin University of Science and Technology,Tianjin,300457,China;[2]Research Center for Fermentation Engineering of Hebei,College of Bioscience and Bioengineering,Hebei University of Science and Technology,Shijiazhang,050000,China;[3]Institute of Chemical Technology and Engineering,Faculty of Chemical Technology,Poznan University of Technology,Berdychowo 4,PL-60695,Poznan,Poland 出　　处：《Particuology》索引2023年第12期,共8页 基　　金：This work was partially supported by the Open Project Program of State Key Laboratory of Food Nutrition and Safety,Tianjin University of Science&Technology(project No.SKLFNS-KF-202203);Dr.J.D.Cui thanks support from the Science and Technology Program of Tianjin,China(project No.20ZYJDJC00080);the international collaboration project(grant No.2020/37/K/ST8/03805). 摘　　要：The enzyme hybrid nanoflower has gained interests in biosensors due to their simple synthesis and high efficiency.In this study,glutamate oxidase(GLOX)and horseradish peroxidase(HRP)hybrid nanoflowers(GLOX&HRP-HNFs)were successfully prepared for the detection of glutamic acid(Glu).The effects of the synthesis conditions on the activity of GLOX&HRP-HNFs were investigated.Results revealed that the maximum activity of GLOX&HRP-HNFs was under 4 mM phosphate radical,2.5 mM MnSO4,0.04 mg/mL GLOX,and 0.16 mg/mL HRP.After immobilization,no significant differences were observed in optimum pH and temperature values of the GLOX and HRP.The GLOX&HRP-HNFs exhibited higher storage stability and resistance to organic solvents than free GLOX and HRP.Additionally,the GLOX&HRP-HNFs maintained 69%of its primary activity after 6 cycles.More important,the GLOX&HRP-HNFs exhibited a good linear range from 1 to 100μM(R^(2)=0.9979)and a low limit of detection(LOD)of 0.59μM for glutamate.These results suggest that the GLOX&HRP-HNFs is a promising candidate for applications in biosensing for the detection of glutamate. 关 键 词：Glutamate oxidase Horseradish peroxidase NANOFLOWERS Glutamate detection