Sub-chronic(Ninety Days)Toxicity Study of Hydroethanolic Leaf Extract of Datura stramonium L.in Rodents

查看全文 作　　者：Abdullahi [1]A.Murtala;Oyinloye [2]E.Oladapo;Aderonke [1]A.Aderionla;Wasiu [3]E.Olooto;Oluwatosin [3]O.Soyinka;Royhan [4]O.Folarin;Farouk [2]A.Oladoja;Oluwatoyin [1]O.Shonde;Luqmon [1]E.Osipitan;Emmanuel [1]B.Adegbe;Julius [1]A.Abolarinwa 机构地区：[1]Department of Pharmacology and Therapeutics,Faculty of Basic Medical Sciences,Obafemi Awolowo College of Health Sciences,Olabisi Onabanjo University,Sagamu Campus,Ogun State 121102,Nigeria;[2]Department of Pharmacology and Toxicology,Faculty of Pharmacy,Olabisi Onabanjo University,Sagamu Campus,Ogun State 121102,Nigeria;[3]Department of Chemical Pathology,Faculty of Basic Medical Sciences,Obafemi Awolowo College of Health Sciences,Olabisi Onabanjo University,Sagamu Campus,Ogun State 121102,Nigeria;[4]Department of Anatomy,Faculty of Basic Medical Sciences,Obafemi Awolowo College of Health Sciences,Olabisi Onabanjo University,Sagamu Campus,Ogun State 121102,Nigeria 出　　处：《Clinical Complementary Medicine and Pharmacology》索引2023年第3卷第3期,共12页 摘　　要：Background:Phyto-medicine represents a vast pool of novel drug development,but understanding their safety requires elaborate,multifaceted approaches,including toxicity studies.Objective:This study investigated the effects of 90 days of oral administration of Datura stramonium(DSE)leaf extract in Rats.Methods:In the oral acute toxicity study,mice were treated with a single oral gavage of DSE at 500,1000,and 2000 mg·kg^(-1)/d,po and observed for signs of acute toxicity for 14 days.In the sub-chronic study,rats were randomized into four Groups(A-D).Group A received distilled water(10 mL·kg^(-1),po)while groups B-D received DSE(10,50 and 250 mg·kg^(-1)/d,po,respectively)orally for 90 days uninterrupted.Animals were weighed weekly,with food and water measured daily and relevant parameters assayed at the end of the 90days administration.Results:In acute toxicity studies,oral administration of up to 2 g·kg^(-1)/d,po of DSE did not elicit any semblance of toxicity or mortality within 24 h to 14 days.In the 90days studies,DSE(250 mg·kg^(-1)/d,po)decreased the body weight,brain weight,and food intake in female rats.DSE(10-250 mg·kg^(-1)/d,po)increased the red blood cell(RBC),packed cell volume(PCV)and hemoglobin(Hb)in both sexes.DSE(10-250 mg·kg^(-1)/d,po)increased the triglycerides(TG),cholesterol and low-density lipoprotein(LDL);and decreased HDL in both sexes.DSE(10-250 mg·kg^(-1)/d,po)increased the white blood cells(WBC)and platelets in female rats.DSE(10-250 mg·kg^(-1)/d,po)decreased the alkaline phosphatase(ALP)and alanine transaminase(ALT)in both studies.Serum urea level was decreased in both sexes.DSE(250 mg·kg^(-1)/d,po)decreased male rats’serum sodium ion levels.Liver,brain,testes and kidney showed severe lesions at 250 mg·kg^(-1)/d,po of the extract.Conclusion:D.stramonium is safe on acute exposure and relatively safe on sub-chronic oral administration.How-ever,prolonged use,especially at high doses,could cause Liver,brain and kidney toxicities;and abnormal lipid metabolism. 关 键 词：Datura stramonium Phyto-medicine Acute toxicity Sub-chronic toxicity Liver enzymes