Reflective ultrathin light-sheet microscopy with isotropic 3D resolutions

查看全文 作　　者：YUE [1]WANG;DASHAN [1]DONG;WENKAI [1]YANG;RENXI [2]HE;MING [3]LEI;KEBIN [1,4,5]SHI 机构地区：[1]State Key Laboratory for Mesoscopic Physics,Department of Physics,Peking University,Beijing 100871,China;[2]State Key Laboratory of Membrane Biology,School of Life Sciences,and Biomedical Pioneering Innovation Center(BIOPIC),Peking University,Beijing 100871,China;[3]School of Physics,Xi’an Jiaotong University,Xi’an 710049,China;[4]Collaborative Innovation Center of Extreme Optics,Shanxi University,Taiyuan 030006,China;[5]Peking University Yangtze Delta Institute of Optoelectronics,Nantong 226010,China 出　　处：《Photonics Research》索引2024年第12卷第2期,共11页 基　　金：National Key Research and Development Program of China(2022YFC3401100,2022YFF0712500);Guangdong Major Project of Basic and Applied Basic Research(2020B0301030009);National Natural Science Foundation of China(12204017,12004012,12004013,12041602,91750203,91850111,92150301);China Postdoctoral Science Foundation(2020M680220,2020M680230);Clinical Medicine Plus X-Young Scholars Project;Peking University;Fundamental Research Funds for the Central Universities;High-performance Computing Platform of Peking University。 摘　　要：Light-sheet fluorescence microscopy(LSFM)has played an important role in bio-imaging due to its advantages of high photon efficiency,fast speed,and long-term imaging capabilities.The perpendicular layout between LSFM excitation and detection often limits the 3D resolutions as well as their isotropy.Here,we report on a reflective type light-sheet microscope with a mini-prism used as an optical path reflector.The conventional high NA objectives can be used both in excitation and detection with this design.Isotropic resolutions in 3D down to300 nm could be achieved without deconvolution.The proposed method also enables easy transform of a conventional fluorescence microscope to high performance light-sheet microscopy. 关 键 词：resolution EXCITATION light