Effects of Rheum tanguticum polysaccharide on TNBS-induced colitis and CD4^+T cells in rats

查看全文 作　　者：[1]LiLiu;Zhi-[1]PengWang;Chang-[2]TaiXu;Bo-[3]RongPan;Qi-[1]BingMei;[4]YinLong;Jia-[4]YunLiu;Si-[1]YuanZhou

机构地区：[1]DepartmentofPharmacology,FourthMilitaryMedicalUniversity,Xi'an710032,ShaanxiProvince,China;[2]Chang-TaiXu,EditorialDepartmentofJournalofFourthMilitaryMedicalU_niversity,Xi’an710032,ShaanxiProvince,China,;[3]DepartmentofOncology,XijingHospital,FourthMilitaryMedicalUniversity,Xi'an710032,ShaanxiProvince,China;[4]DepartmentofTradltlonalChineseMedicine,FourthMilitaryMedicalUniversity,Xi’an710032,ShaanxiProvince,China

出　　处：《World Journal of Gastroenterology》索引2003年第9卷第10期,共5页

基　　金：the National Natural Science Foundation of China,No 30100239 摘　　要：AIM: To study the effects of Rheum tanguticum polysaccharide-1 (RTP-1) on ulcerative colitis in rats induced by 2, 4, 6-trinitrophene sulphonic acid (TNBS) and their possible mechanism.METHODS: RTP1 (200 mg@kg-1, ig) extracted from Rheum tanguticum Maxim. Ex Regel was administrated to rats with colitis induced by TNBS for 5 d, 7 d, 10 d and 14 d,respectively. The effects of RTP1 and dexamethasone (DX,0.2 mg@kg-1, ig) were contrastively investigated. The MPO level and SOD activity were determined by chromatometry.The expansion and protein expression of CD4+T lymphocytes isolated from colon mucosae and mesenteric lymph nodes of colitis rats were performed by immunohistochemical analysis and Western-blot methods.RESULTS: Treatments of RTP1 (200 mg@kg-1, ig) significantly reduced diarrhea, mortality, colon mass, ulcer areas and MPO level in colon mucosae on days 5, 7, 10 and 14 (5.2±1.4,5.4±0.7, 5.2±1.8, P<0.05.3.4±0.8, P<0.01. 16.1±12.1,P<0.01.31.8±8.6, 17.7±5.3, 12.7±4.1, P<0.05). The effectsof RTP1 were similar to those noted above in DX group, but there were no immunosupressive effects of DX in RTP-1group, such as body mass loss, thymus and spleen atrophy.The decreased number and down-regulated protein levels of CD4+T cells isolated from the colon of colitis rats treated with RTP1 were found.CONCLUSION: RTP1 shows significantly protective effects but lower side effects on rats with colitis induced by TNBS.The mechanism may be due to the resistance to over expansion of CD4. 关键词：T淋巴细胞 CD4+ 大肠炎 RTP.1 药理作用动物实验

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Effects of Rheum tanguticum polysaccharide on TNBS-induced colitis and CD4^+T cells in rats

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耦合文献(340)

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