Different Thermostability of Skeletal Muscle Glyceraldehyde-3-phosphate Dehydrogenase from Hibernating and Euthermic Jerboa (Jaculus orientalis)

查看全文 作　　者：IDDAR [1]Abdelghani;CAMPOS Luis [1]A.;SANCHO [1]Javier;SERRANO [1]Aurelio;SOUKRI [1]Abdelaziz 机构地区：[1]Laboratoire de Biochimie,Departamento de Bioquímica y Biología Molecular y Celular,Departamento de Bioquímica y Biología Molecular y Celular,Instituto de Bioquímica Vegetal y Fotosíntesis,Laboratoire de Biochimie Unitè de Gènie Enzymatique et Biologie Molèculaire, Facultè des Sciences-Ain Chock, Casablanca, Morocco, Universidad de Zaragoza, Zaragoza, Spain , Universidad de Zaragoza, Zaragoza, Spain , CSIC-Universidad de Sevilla, Sevilla, Spain ,Unitè de Gènie Enzymatique et Biologie Molèculaire, Facultè des Sciences-Ain Chock, Casablanca, Morocco 出　　处：《生物化学与生物物理学报》索引2003年第35卷第10期,共6页 基　　金：ThisworkwassupportedbyAECI (Spain) ,acollaborativegrantoftheAndalusianRegionalGovernment (ConvenioColaboracinUniv .Marroques,grupoPAICVI 2 61) ,andgrantsBMC 2 0 0 1 2 5 2andP12 0 2 0 0 1fromtheSpanishMinistryofEducationandtheDGA 摘　　要：In previous study, we demonstrated that the specific activity of D-glyceraldehyde-3-phosphate dehydrogenase (GAPDH, EC 1.2.1.12) in skeletal muscle of induced hibernating jerboa (hibernating GAPDH) was 3-4 folds lower than that of the one in the skeletal muscle of the euthermic jerboa (euthermic GAPDH). A significant decrease in both GAPDH protein and GapC mRNA levels occurs when hibernating, but the purified hibernating GAPDH is less active than the euthermic GAPDH. To investigate the physico-chemical basis of this lower activity, the behaviour during thermal inactivation of skeletal muscle GAPDH from hibernating and euthermic tissues was examined by a variety of spectroscopic techniques, including fluorescence emission, circular dichroism and ultraviolet absorption. A clear resistance to thermal denaturation was observed in the hibernating GAPDH compared with the euthermic GAPDH. The different temperature of denaturation found in these proteins by both fluorimetry and circular dichroism indicates that there might exist conformational changes of GAPDH upon hibernation that could affect the stability of this enzyme. 关 键 词：跳鼠 冬眠 发热 热能 骨骼肌 3-磷酸甘油醛脱氢酶