Establishment of in Vitro Regeneration System of Bitter Melon (Momordica Charantia L. )

查看全文 作　　者：杨满业 [1];Zhao Maojun [2];Zeng Yu [1];Lan Liqong [1];Chen Fang [1] 机构地区：[1]College of Life Sciences, Sichuan University, Chendu 610064,P.R.China;[2]Department of Fundamental Subject, Sichuan Agricultural University, Ya'an 625000,P.R.China; 出　　处：《High Technology Letters》索引2004年第10卷第1期,共5页 摘　　要：Explants of bitter melon can produce three types of callus: green, yellow green and fragile yellow callus. During the period of inducing callus, the explants are not sensitive to the kinds and proportions of phytohormone. The rate of callus inductivity is about 90.0%. However, in the process of callus differentiating adventitious bud, the kind, proportion and quantity of phytohormone and the type of callus made different result. The adventitious buds were induced successfully on MS medium+6-BA (N6-benzyladenine) 4.0 mg l-1+KT(kinetin) 2.0 mg l-1) by yellow green callus. The frequency was about 66.7 %. Yellow callus did not differentiated adventitious bud. The frequency of green callus differentiation was very low (<15.0 %) even under the most suitable conditions. The MS + ZT (zeatin) 5.0 mgl-1 +KT 0.5 mgl-1 medium was suitable for the proliferation of bud and three weeks later, the coefficient of proliferation was about 5-6. The 1/2 MS + ZT 0.02 mgl-1 or 1/2 MS media were suitable for in vitro rooting of shoot, the shoot on them could produce 6-7 new roots in three weeks. After plantation test, the survival rate of tube plantlets was about 70% and their characteristics were the same as those from seed by field test. 关 键 词：苦瓜 Vitro重建 愈合组织 分芽繁殖 外植体 植物激素 诱导