Effect of cyclin G2 on proliferative ability of SGC-7901 cell

查看全文 作　　者：[1]JieLiu;Ze-[1]ShiCui;[2]YangLuo;[2]LiJiang;Xiao-[2]HuiMan;[2]XueZhang

机构地区：[1]ExperimentalTechnologyCenter,ChinaMedicalUniversity,Shenyang110001,LiaoningProvince,China;[2]MedicalGeneGroupofCellBiologyLaboratory,KeyLaboratoryoftheMinistryofHealth,ChinaMedicalUniversity,Shenyang110001,LiaoningProvince,China

出　　处：《World Journal of Gastroenterology》索引2004年第10卷第9期,共4页

基　　金：Supported by a grant for Distinguished Young Teachers of HigherEducation of the Ministry of Education of the Teaching and ResearchEncouragement Plan 摘　　要：AIM: To study the effect of cyclin G2 on proliferation of gastric adenocarcinoma cell line-SGC-7901 cell in vitro.METHODS: By use of cation lipofectamine transfection reagent,the pIRES-G2 and pIRESneo plasmids were transferred into SGC-7901cell line. Anticlones were selected by G418. Positive clones were observed and counted using Giemsa staining.Cell proliferative ability was assayed by Ml-r.RESULTS: (1) The done number of pIRES-G2 group decreased,clone volume reduced. The number of cell clones in pIRESneo group was 87+3, that of pIRES-G2 group was 53+4,occupying 60.1% of pIRESneo group, there was significant difference obviously (P<0.01, t=15.45). (2) The average absorbance of clone cell obtained by stable transfection of pIRES-G2 at 570 nm was 1.6966+0.2125, the average absorbance of clone cell obtained by stable transfection of pIRESneo at 570 nm was 2.1182+0.3675, there was significant difference between them (P<0.01, t=3.412).CONCLUSION: Cyclin G2 can inhibit SGC-7901cell proliferative ability obviously, it may be a negative regulator in cell cycle regulation. 关键词：细胞周期蛋白G2 SGC-7901细胞胃腺癌细胞增殖 MTT法

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Effect of cyclin G2 on proliferative ability of SGC-7901 cell

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