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| 1 | Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells显示文摘BACKGROUND Human adipose-derived stromal/stem cells(hASCs)are one of the most useful types of mesenchymal stromal/stem cells,which are adult multipotent cells with great therapeutic potential for the treatment of several diseases.However,for successful clinical application,it is critical that high-quality cells can be obtained.Diverse factors seem to be able to influence cell quality and performance,especially factors related to donors’intrinsic characteristics,such as age.Nevertheless,there is no consensus regarding this characteristic,and there is conflicting information in the literature.AIM To investigate the growth kinetics and differentiation potential of adipose-derived stem cells isolated from the lipoaspirates of elderly and young donors.METHODS hASCs were harvested from liposuctioned adipose tissue obtained from female donors(aged 20-70 years).Cells were distributed into two groups according to age range:old hASCs(oASCs,≥55 years,n=9)and young hASCs(yASCs,≤35 years,n=9).For each group,immunophenotypic characterization was performed by flow cytometry.Population doubling time was assessed over seven days.For adipogenic potential evaluation,lipid deposits were assessed after 7 d,14 d and 21 d of adipogenic induction.Osteogenic potential was verified by analyzing cell mineralization after 14 d,21 d and 28 d of osteogenic induction.mRNA expression of PPARγ2,CEBPA and Runx2 were detected by quantitative reverse transcription polymerase chain reaction.RESULTS hASCs were successfully obtained,cultured,and grouped according to their age:yASCs(26.33±4.66 years old)and oASCs(64.78±4.58 years old).After maintenance of the cells in culture,there were no differences in morphology between cells from the young and old donors.Additionally,both groups showed classical immunophenotypic characteristics of mesenchymal stem/stromal cells.The average doubling time indicated that yASCs(4.09±0.94 d)did not significantly differ from oASCs(4.19±1.29 d).Concerning differentiation potential,after adipogenic and osteogenic induction,yASCs and oASCs were able to differentiate to greater levels than the noninduced control cells.However,no differences were found in the differentiation efficiency of yASCs and oASCs in adipogenesis or osteogenesis.Additionally,the mRNA expression of PPARγ2,CEBPA and Runx2 were similar in yASCs and oASCs.CONCLUSION Our findings suggest that age does not seem to significantly affect the cell division or adipogenic or osteogenic differentiation ability of adipose-derived stem cells isolated from lipoaspirates. | Cintia DS Horinouchi María Julia Barisón Anny W Robert Crisciele Kuligovski Alessandra M Aguiar BrunoDallagiovanna | 2020 | World Journal of Stem Cells2020，12，12： | 2 |
| 2 | Slowmagic-angle coil spinning: a high-sensitivity and high-resolution NMR strategy for microscopic biologicalspecimens显示文摘 | WONG A AGUIAR P M SAKELLARIOU D | 2010 | Magnetic Resonance in Medicine2010，63，2： | 1 |
| 3 | Friedel-Crafts acylaiton of anisole with acetic anhydride over silica-supported hetempolyphostungstic acid (HPW/SiO2 )显示文摘 | CARDOSO L A M ALVES W It GONZAGA A R E AGUIAR L M G ANDRADE H M C | 2004 | J Mol Catal A2004，209，12： | 1 |
| 4 | Internal suture technique for improving projection and stability in secondary gluteoplasty显示文摘 | JAIMOVICH C A ALMEIDA M W AGUIAR L F | 2010 | Aesthet Surg J2010，30，3： | 1 |
| 5 | Encapsulation of insulin-cyelodextrin complex in PLGA microspheres: a new approach for prolonged puhnonary insulin delivery显示文摘 | Aguiar M M Rodrigues J M Silva Cunba A | 2004 | Microencapsul2004，21，5： | 1 |
| 6 | Best cases from the AFIP:congenital intracranial teratoma显示文摘 | SANDOW B A DORY C E AGUIAR M A | 2004 | Radiographics2004，24，4： | 1 |
| 7 | Antimalarial activity and mechanisms of action of two novel 4- aminoquinolines against chloroquine-resistant parasites 显示文摘 | AGUIAR A C C SANTOS R D M FIGUEIREDO F J B | 2012 | PLoS One2012，7，37： | 1 |
| 8 | Microwave Promoted Suzuki Reactions between Aroyl Chlorides and Boronic Acids Catalyzed by Het- erogeneous and Homogeneous Phosphine-free Palla- dium Catalysts 显示文摘 | DANIELA DE L M AGUIAR L C ANTUNES O A | 2011 | J of Organomet Chem2011，696，1516： | 1 |
| 9 | Dissimilar differentiation of mesenchymal stem ceils from bone marrow, umbilical cord blood, and adipose tissue显示文摘 | Rebelatto CK Aguiar A M Moretao M P | 2008 | Exp Biol Med (May wood)2008，233，7： | 1 |
| 10 | Strain behavior of lanthanum modified BiFeO3 thin films prepared via soft chemical method显示文摘 | SIMOES A Z AGUIAR E C GOZALEZ A H M | 2008 | J Appl Phys2008，104，10： | 1 |
| 11 | Assignment of the steroid receptor coactivator-1(SRC-1)gene to human chromosome band 2p23显示文摘 | Carapeti M Aguiar RC Chase A | 1998 | Genomics1998，52，2： | 1 |
| 12 | New approaches in antimalarial drug discovery and develop- ment:A review 显示文摘 | AGUIAR A C C ROCHA E M D SOUZA N B D | 2012 | Mere Inst Oswaldo Cruz2012，107，7： | 1 |
| 13 | Molecular fragmentation of wheat-germ agglutinin induced by food irradiation reduces its allergenicity in sensitised mice显示文摘 | Vaz A F M Souza M P Carneiro-da-Cunha M G Medeiros P L Melo A M M A Aguiar J S Silva T G Silva-Lucca R A Oliva M L V Correia M T S | 2012 | Food Chemistry2012，132，2： | 1 |
| 14 | Effect of process parameters on the characteristics of porous calcium phosphate ceramics for bone tissue scaffolds 显示文摘 | De Oliverira J F De Aguiar P F Rossi A M | 2003 | Int Soc Art Org2003，27，3： | 1 |
| 15 | Enrichment of whole wheat flaxseed bread with flaxseed oil 显示文摘 | Aguiar A C D Boroski M Monteiro A R G | 2011 | Journal of Food Processing and Preservation2011，35，5： | 1 |
| 16 | Automatic bottom-following for underwater robotic vehicles 显示文摘 | Adhami-Mirhosseini A Yazdanpanah M J Aguiar A P | 2014 | Automatica2014，50，8： | 1 |
| 17 | Characterization of glycosaminoglycans in tubular epithelial cells: calcium oxalate and oxalate ions effects 显示文摘 | Borges F T Michelacci Y M Aguiar J A | 2005 | Kid Internation2005，68，4： | 1 |
| 18 | Changes in isoflavone profiles of soybean treated with gamma irradiation 显示文摘 | Aguiar C L Baptista A S Walder J M | 2009 | Int J Food Sci Nutr2009，60，5： | 1 |
| 19 | Spectral analysis and the dynamic response of complex networks显示文摘 | De Aguiar M A M Bar-Yam Y | 2005 | Phys Rev E2005，71，01： | 1 |
| 20 | Effects of simulated acid rain on the foliar micromorphology and anatomy of tree tropical species显示文摘 | Sant'Anna-Santos B F Silva L C Azevedo A A Araujo J M Aires E F Silva E A M Aguiar R | 2006 | Enviromental and Exprimental Botany2006，58，13： | 1 |
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