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| 1 | 2,4,6-trinitrobenzene sulfonic acid-induced chronic colitis with fibrosis and modulation of TGF-β1 signaling显示文摘AIM:To investigate whether targeting proteasome might reverse intestinal fibrosis in rats.METHODS:Chronic colitis was induced in rats by repeated administration of increasing dose of2,4,6-trinitrobenzene sulfonic acid(TNBS,15,30,45,60,60,60 mg)by rectal injection for 6 wk(from day0 to day 35),while control rats received the vehicle.TNBS+bortezomib(BTZ)rats received intraperitoneal injections of BTZ twice weekly(from day 37 to day44)at a dose of 25 mg/kg,whereas the control and TNBS groups received the same amount of the vehicle.Histologic scoring of inflammation and fibrosis was performed.Colonic production of transforming growth factor(TGF)-βwas measured by ELISA.Colon fibrosisrelated proteins such as phospho-p38,phosphoSMAD2/3,Akt and peroxisome proliferator activated receptorγ(PPARγ)were studied by western blot.Expression of the tight junction proteins,occludin and claudin-1,were assessed by Western blot.Colon proteasome activities(chymotrypsin-like and trypsinlike activities)were assessed.RESULTS:TNBS-treated rats had a higher colon weight/length ratio compared to control rats(P<0.01).Furthermore,fibrosis and inflammation scores were higher in TNBS-treated rats compared to control rats(P<0.01 for both).Colonic production of TGF-βproduction tended to be higher in TNBS-treated rats(P<0.06).Fibrosis-related proteins such as phospho-p38,phospho-SMAD2/3,and PPARγwere significantly higher in TNBS-treated rats compared to control rats(all P<0.05).TNBS rats had a higher expression of Akt compared to control rats(P<0.01).Tight junction proteins were modified by repeated TNBS challenge:colon occludin expression rose significantly(P<0.01),whereas claudin-1 expression fell(P<0.01).Bortezomib inhibition significantly decreased chymotrypsin-like activity(P<0.05),but had no significant effect on trypsin-like activity(P>0.05).In contrast,bortezomib had no effect on other studied parameters such as fibrosis score,TGF-βsignaling,or tight junction expression(P>0.05 for all).CONCLUSION:Rats with TNBS-induced chronic colitis exhibited colon fibrosis associated with higher TGF-βsignaling.Proteasome inhibition by bortezomib had no effect on fibrosis in our experimental conditions. | Emilien Loeuillard Julien Bertrand Anni Herranen Chloé Melchior Charlène Guérin Mo?se Co?ffier Moutaz Aziz Pierre Déchelotte Guillaume Savoye Rachel Marion-Letellier | 2014 | World Journal of Gastroenterology2014，20，48： | 4 |
| 2 | A high-density, multi-parental SNP genetic map on apple validates a new mapping approach for outcrossing species显示文摘Quantitative trait loci(QTL)mapping approaches rely on the correct ordering of molecular markers along the chromosomes,which can be obtained from genetic linkage maps or a reference genome sequence.For apple(Malus domestica Borkh),the genome sequence v1 and v2 could not meet this need;therefore,a novel approach was devised to develop a dense genetic linkage map,providing the most reliable marker-loci order for the highest possible number of markers.The approach was based on four strategies:(i)the use of multiple full-sib families,(ii)the reduction of missing information through the use of HaploBlocks and alternative calling procedures for single-nucleotide polymorphism(SNP)markers,(iii)the construction of a single backcross-type data set including all families,and(iv)a two-step map generation procedure based on the sequential inclusion of markers.The map comprises 15417 SNP markers,clustered in 3 K HaploBlock markers spanning 1267 cM,with an average distance between adjacent markers of 0.37 cM and a maximum distance of 3.29 cM.Moreover,chromosome 5 was oriented according to its homoeologous chromosome 10.This map was useful to improve the apple genome sequence,design the Axiom Apple 480 K SNP array and perform multifamily-based QTL studies.Its collinearity with the genome sequences v1 and v3 are reported.To our knowledge,this is the shortest published SNP map in apple,while including the largest number of markers,families and individuals.This result validates our methodology,proving its value for the construction of integrated linkage maps for any outbreeding species. | Erica A Di Pierro Luca Gianfranceschi Mario Di Guardo Herma JJ Koehorst-van Putten Johannes W Kruisselbrink Sara Longhi Michela Troggio Luca Bianco Hélène Muranty Giulia Pagliarani Stefano Tartarini Thomas Letschka Lidia Lozano Luis Larisa Garkava-Gustavsson Diego Micheletti Marco CAM Bink Roeland E Voorrips Ebrahimi Aziz Riccardo Velasco François Laurens W Eric van de Weg | 2016 | Horticulture Research2016，3，1： | 3 |
| 3 | Plumbagin,a medicinal plant-derived naphthoquinone,is a novel inhibitor of the growth and invasion of hormone-refractory prostate cancer显示文摘 | Aziz MH Dreckschmidt NE Verma AK | | 0，，： | 1 |
| 4 | Plumbagin,a medicinal plant-derived naphthoquinone,is a novel inhibitor of the growth and invasion of hormone-refractory prostate cancer显示文摘 | Aziz MH Dreckschmidt NE Verma AK | 2008 | Cancer Res2008，68，21： | 1 |
| 5 | Differential regula-tion of c-Myb-induced transcription activation by a phosphorylation site inthe negative regulatory domain显示文摘 | MIGLARESE M R RICHARDSON A F AZIZ Ne t al | 1996 | Journal of Biological Chemistry1996，271，22： | 1 |
| 6 | Plumbagin,a medici-nal plant-derived naphthoquinone,is a novel inhibitor of the growth and invasion of hormone-refractory prostate cancer显示文摘 | Aziz MH Dreckschmidt NE Verma AK | 2008 | Cancer Res2008，68，21： | 1 |
| 7 | Plumbagin, a medicinal plant-derived naphthoquinone, is a novel inhibitor of the growth and invasion of hormone-refractory prostate cancer 显示文摘 | Aziz MH Dreckschmidt NE Verma AK | 2008 | Cancer Res2008，68，： | 1 |
| 8 | Protein kinase cvarepsilon inhibits UVR-induced expression of FADD,an adaptor protein,linked to both fas-and TNFR1-mediated apoptosis显示文摘 | Aziz MH Sundling KE Dreckschmidt NE | 2009 | J Invest Dermatol2009，129，8： | 1 |
| 9 | SPECT-computed tomography in rats with TNBS-induced colitis: A first step toward functional imaging显示文摘AIM To assess the feasibility of SPECT-computed tomography(CT) in rats with trinitrobenzene sulfonic acid(TNBS)-induced acute colitis and confront it with model inflammatory characteristics.METHODS Colitis was induced in Sprague-Dawley rats by intrarectal injection of TNBS(n = 10) while controls received vehicle(n = 10). SPECT-CT with intravenous injection of 10 MBq of 67Ga-Citrate was performed at day 2. SPECT-CT criteria were colon wall thickness andmaximal wall signal intensity. Laboratory parameters were assessed: colon weight:length ratio, colon cyclooxygenase-2 expression by western blot and histological inflammatory score.RESULTS Colon weight/length ratio, colon COX-2 expression and histological inflammatory score were significantly higher in the TNBS group than in the control group(P = 0.0296, P < 0.0001, P = 0.0007 respectively). Pixel max tend to be higher in the TNBS group than in the control group but did not reach statistical significance(P = 0.0662). Maximal thickness is significantly increased in the TNBS group compared to the control group(P = 0.0016) while colon diameter is not(P = 0.1904). Maximal thickness and colon diameter were correlated to colon COX-2 expression(P = 0.0093, P = 0.009 respectively) while pixel max was not(P = 0.22). Maximal thickness was significantly increased when inflammation was histologically observed(P = 0.0043) while pixel max and colon diameter did not(P = 0.2452, P = 0.3541, respectively).CONCLUSION SPECT-CT is feasible and easily distinguished control from colitic rats. | Rachel Marion-Letellier Pierre Bohn Romain Modzelewski Pierre Vera Moutaz Aziz Charlène Guérin Guillaume Savoye Céline Savoye-Collet | 2017 | World Journal of Gastroenterology2017，23，2： | 0 |
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