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1篇 您的检索式:作者名="Ajay Kumar Mahato"
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1Utility of Triti-Map for bulk-segregated mapping of causal genes and regulatory elements in Triticeae显示文摘Triticeae species,including wheat,barley,and rye,are critical for global food security.Mapping agronomically important genes is crucial for elucidating molecular mechanisms and improving crops.However,Triticeae includes many wild relatives with desirable agronomic traits,and frequent introgressions occurred during Triticeae evolution and domestication.Thus,Triticeae genomes are generally large and complex,making the localization of genes or functional elements that control agronomic traits challenging.Here,we developed Triti-Map,which contains a suite of user-friendly computational packages specifically designed and optimized to overcome the obstacles of gene mapping in Triticeae,as well as a web interface integrating multi-omics data from Triticeae for the efficient mining of genes or functional elements that control particular traits.The Triti-Map pipeline accepts bothDNA and RNAbulk-segregated sequencing data as well as traditional QTL data as inputs for locating genes and elucidating their functions.We illustrate the usage of Triti-Map with a combination of bulk-segregated ChIP-seq data to detect a wheat disease-resistance gene with its promoter sequence that is absent from the reference genome and clarify its evolutionary process.We hope that Triti-Map will facilitate gene isolation and accelerate Triticeae breeding.Fei Zhao Shilong Tian Qiuhong Wu Zijuan Li Luhuan Ye Yili Zhuang Meiyue Wang Yilin Xie Shenghao Zou Wan Teng Yiping Tong Dingzhong Tang Ajay Kumar Mahato Moussa Benhamed Zhiyong Liu Yijing Zhang 2022Plant Communications2022,3,4:1
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