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| 1 | Anti-inflammatory activity of probiotic Bifidobacterium: Enhancement of IL-10 production in peripheral blood mononuclear cells from ulcerative colitis patients and inhibition of IL-8 secretion in HT-29 cells显示文摘AIM: To determine the anti-inflammatory activity of probiotic Bifidobacteria in Bifidobacteria-fermented milk (BFM) which is effective against active ulcerative colitis (UC) and exacerbations of UC, and to explore the immunoregulatory mechanisms. METHODS: Peripheral blood mononuclear cells (PBMNC) from UC patients or HT-29 cells were co-cultured with heat-killed probiotic bacteria or culture supernatant of Bifidobacterium breve strain Yakult (BbrY) or Bifidobacterium bifidum strain Yakult (BbiY) to estimate the amount of IL-10 or IL-8 secreted. RESULTS: Both strains of probiotic Bifidobacteria contained in the BFM induced IL-10 production in PBMNC from UC patients, though BbrY was more effective than BbiY. Conditioned medium (CM) and DNA of both strains inhibited IL-8 secretion in HT-29 cells stimulated with TNF-α, whereas no such effect was observed with heat- killed bacteria. The inhibitory effect of CM derived from BbiY was greater than that of CM derived from BbrY. DNAs of the two strains had a comparable inhibitory activity against the secretion of IL-8. CM of BbiY induced a repression of IL-8 gene expression with a higher expression of IκB-ζ mRNA 4 h after culture of HT-29 cells compared to that in the absence of CM.CONCLUSION: Probiotic Bifidobacterium strains in BFM enhance IL-10 production in PBMNC and inhibit IL-8 secretion in intestinal epithelial cells, suggesting that BFM has anti-inflammatory effects against ulcerative colitis. | Akemi Imaoka Tatsuichiro Shima Kimitoshi Kato Shigeaki Mizuno Toshiki Uehara Satoshi Matsumoto Hiromi Setoyama Taeko Hara Yoshinori Umesaki | 2008 | World Journal of Gastroenterology2008，14，16： | 37 |
| 2 | Prognostic value of KRAS and BRAF mutations in curatively resected colorectal cancer显示文摘AIM: To investigate the prognostic role of KRAS and BRAF mutations after adjustment for microsatellite instability(MSI) status in Japanese colorectal cancer(CRC) population.METHODS: We assessed KRAS and BRAF mutations and MSI status in 813 Japanese patients with curatively resected, stage Ⅰ-Ⅲ CRC and examined associations of these mutations with disease-free survival(DFS) and overall survival(OS) using uni- and multivariate Cox proportional hazards models.RESULTS: KRAS and BRAF mutations were detected in 312(38%) of 812 and 40(5%) of 811 tumors, respectively. KRAS mutations occurred more frequently in females than in males(P = 0.02), while the presence of BRAF mutations was significantly associated with the female gender(P = 0.006), proximal tumor location(P < 0.001), mucinous or poorly differentiated histology(P < 0.001), and MSI-high tumors(P < 0.001). After adjusting for relevant variables, including MSI status, KRAS mutations were associated with poorer DFS(HR = 1.35; 95%CI: 1.03-1.75) and OS(HR = 1.46; 95%CI: 1.09-1.97). BRAF mutations were poor prognostic factors for DFS(HR = 2.20; 95%CI: 1.19-4.06) and OS(HR = 2.30; 95%CI: 1.15-4.71). Neither the BRAF by MSI interaction test nor the KRAS by MSI interaction test yielded statistically significant results for DFS and OS.CONCLUSION: KRAS and BRAF mutations are associated with inferior survival, independent of MSI status, inJapanese patients with curatively resected CRC. | Shigenori Kadowaki Miho Kakuta Shuhei Takahashi Akemi Takahashi Yoshiko Arai Yoji Nishimura Toshimasa Yatsuoka Akira Ooki Kensei Yamaguchi Keitaro Matsuo Kei Muro Kiwamu Akagi | 2015 | World Journal of Gastroenterology2015，21，4： | 14 |
| 3 | Circulating RNAs as new biomarkers for detecting pancreatic cancer显示文摘Pancreatic cancer remains difficult to treat and has a high mortality rate.It is difficult to diagnose early mainly due to the lack of screening imaging modalities and specific biomarkers.Consequently,it is important to develop biomarkers that enable the detection of early stage tumors.Emerging evidence is accumulating that tumor cells release substantial amounts of RNA into the bloodstream that strongly resist RNases in the blood and are present at sufficient levels for quantitative analyses.These circulating RNAs are upregulated in the serum and plasma of cancer patients,including those with pancreatic cancer,compared with healthy controls.The majority of RNA biomarker studies have assessed circulating microRNAs(miRs),which are often tissue-specific.There are few reports of the tumorspecific upregulation of other types of small noncoding RNAs(ncRNAs),such as small nucleolar RNAs and Piwi-interacting RNAs.Long ncRNAs(IncRNAs),such as HOTAIR and MALAT1,in the serum/plasma of pancreatic cancer patients have also been reported as diagnostic and prognostic markers.Among tissuederived RNAs,some miRs show increased expression even in pre-cancerous tissues,and their expression profiles may allow for the discrimination between a chronic inflammatory state and carcinoma.Additionally,some miRs and IncRNAs have been reported with significant alterations in expression according to disease progression,and they may thus represent potential candidate diagnostic or prognostic biomarkers that may be used to evaluate patients once detection methods in peripheral blood are well established.Furthermore,recent innovations in high-throughput sequencing techniques have enabled the discovery of unannotated tumor-associated ncRNAs and tumor-specific alternative splicing as novel and specific biomarkers of cancers.Although much work is required to clarify the release mechanism,origin of tumor-specific circulating RNAs,and selectivity of carrier complexes,and technical advances must also be achieved,such as creating a consensus normalization protocol for quantitative data analysis,circulating RNAs are largely unexplored and might represent novel clinical biomarkers. | Takahiro Kishikawa Motoyuki Otsuka Motoko Ohno Takeshi Yoshikawa Akemi Takata Kazuhiko Koike | 2015 | World Journal of Gastroenterology2015，21，28： | 8 |
| 4 | Diagnostic and therapeutic application of noncoding RNAs for hepatocellular carcinoma显示文摘Micro RNAs(mi RNAs) are small,noncoding RNA molecules that regulate gene expression posttranscriptionally,targeting thousands of messenger RNAs. Long noncoding RNAs(lnc RNAs),another class of noncoding RNAs,have been determined to be also involved in transcription regulation and translation of target genes. Since deregulated expression levels or functions of miR NAs and lncR NAs in hepatocellular carcinoma(HCC) are frequently observed,clinical use of noncoding RNAs for novel diagnostic and therapeutic applications in the management of HCCs is highly and emergently e xpe c t e d. H e r e,we s ummar iz e r e c e nt f indings regarding deregulated mi RNAs and lnc RNAs for their potential clinical use as diagnostic and prognostic biomarkers of HCC. Specifically,we emphasize the deregulated expression levels of such noncoding RNAs in patients' sera as noninvasive biomarkers,a field that requires urgent improvement in the clinical surveillance of HCC. Since nucleotide-based strategies are being applied to clinical therapeutics,we further summarize clinical and preclinical trials using oligonucleotides involving the use of miR NAs and small interfering RNAs against HCC as novel therapeutics. Finally,we discuss current open questions,which must be clarified in the near future for realistic clinical applications of these new strategies. | Chikako Shibata Motoyuki Otsuka Takahiro Kishikawa Motoko Ohno Takeshi Yoshikawa Akemi Takata Kazuhiko Koike | 2015 | World Journal of Hepatology2015，7，1： | 7 |
| 5 | Novel therapeutic approaches for hepatitis B virus covalently closed circular DNA显示文摘Hepatitis B virus(HBV) infection is a major global health problem. Although current therapies, such as the use of nucleos(t)ide analogs, inhibit HBV replication efficiently, they do not eliminate covalently closed circular DNA(ccc DNA), which persists in hepatocyte nuclei. As HBV ccc DNA is a viral transcription template, novel therapeutic approaches to directly target HBV ccc DNA are necessary to completely eradicate persistent HBV infections. HBV ccc DNA levels in HBV-infected human liver cells are extremely low; thus, more reliable and simple measurement methods are needed to correctly monitor their levels during therapeutic treatment. Although reverse transcription-polymerase chain reaction or Southern blot procedures are currently used in research studies, these methods are not completely reliable and are also time-consuming and labor-intensive. Genome editing technologies, such as zinc finger nucleases, transcription activator-like effector nucleases, and the clustered regularly interspaced short palindromic repeats/Cas9(CRISPR/Cas9) system, which are designed to target specific DNA sequences, represent highly promising potential therapeutic tools. In particular, the CRISPR/Cas9 system is an easily customizable sequencespecific nuclease with high flexibility and may be the most feasible approach to target HBV ccc DNA. Further research to develop easier, safer, and more effective protocols should be pursued. | Motoko Ohno Motoyuki Otsuka Takahiro Kishikawa Takeshi Yoshikawa Akemi Takata Kazuhiko Koike | 2015 | World Journal of Gastroenterology2015，21，23： | 5 |
| 6 | Induction of Intestinal Th17 Cells by Segmented Filamentous Bacteria显示文摘 | Ivaylo I. Ivanov Koji Atarashi Nicolas Manel Eoin L. Brodie Tatsuichiro Shima Ulas Karaoz Dongguang Wei Katherine C. Goldfarb Clark A. Santee Susan V. Lynch Takeshi Tanoue Akemi Imaoka Kikuji Itoh Kiyoshi Takeda Yoshinori Umesaki Kenya Honda Dan R. Littma | 2009 | Cell2009，，3： | 3 |
| 7 | Family-based association study of ZNF533, DOCK4 and IMMP2L gene polymorphisms linked to autism in a northeastern Chinese Han population显示文摘研究目的:ZNF533、DOCK4和IMMP2L在大脑发育过程中起到非常重要的作用,是孤独症研究的候选基因。高加索人群的研究结果发现,ZNF533、DOCK4和IMMP2L基因的5个单核苷酸多态性(SNP)位点与孤独症高度相关。为了探讨上述位点是否与中国孤独症发生相关,我们开展了东北汉族孤独症的核心家系研究。创新要点:孤独症候选基因多态位点的研究结果通常很难得到重复。本研究首次在中国东北汉族人群中验证了与高加索人群孤独症密切相关的候选位点。这一结果对孤独症的研究具有重要指导意义。研究方法:利用SNaPshot的方法,检测了中国东北汉族370个核心家系中ZNF533(rs11885327、rs1964081)、DOCK4(rs2217262)和IMMP2L(rs12537269、rs1528039)的分布情况,利用传递不平衡检验(TDT)分析了这些多态位点与孤独症发生的相关性。重要结论:ZNF533和DOCK4基因多态性与中国北方汉族孤独症发生存在显著关联。 | Shuang LIANG Xue-lai WANG Ming-yang ZOU Han WANG Xue ZHOU Cai-hong SUN Wei XIA Li-jie Wu TakashiX.FUJISAWA Akemi TOMODA | 2014 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2014，15，3： | 2 |
| 8 | High b-value diffusion-weighted MRI for detecting gallbladder carcinoma: preliminary study and results显示文摘 | Reiji Sugita Tetsuro Yamazaki Akemi Furuta Kei Itoh Naotaka Fujita Shoki Takahashi | 2009 | European Radiology2009，，7： | 2 |
| 9 | Acetylenes from Cirsium japon - icum 显示文摘 | Yoshihisa Takashi Takeshi Okuyama Akemi Masuda | 1990 | Phytochemistry1990，29，12： | 1 |
| 10 | Surface-enhanced Raman spectroscopy of DOPA-eontaining peptides related to adhesive protein of marine mussel Mytilus edulis 显示文摘 | Akemi Ooka A Garrell R L | 2000 | Biopolymers2000，57，2： | 1 |
| 11 | B6zier curve and surface fitting of 3D point clouds through genetic algorithms显示文摘 | AKEMI G ANDRes I ANGEL C | 2007 | Lecture Notes in Computer Science2007，4706，： | 1 |
| 12 | Evaluation and indications of ultrasound-guided vacuum-assisted core needle breast biopsy显示文摘 | Satoko Nakano Haruko Sakamoto Masahiko Ohtsuka Akemi Mibu Hitomi Sakata Masahiro Yamamoto | 2007 | Breast Cancer2007，，3： | 1 |
| 13 | Immunochemieal determinant and serological specificity of Candida krusei 显示文摘 | Akemi N Takako S Yoshimura F | 1982 | Molecular Immunology1982，19，3： | 1 |
| 14 | Urinary levels of monocyte chemoattractant protein-1 (MCP-1) and Interleukin-8 (IL-8),and Renal injure in Patientswith Type 2 Diabetic Nephropathy显示文摘 | Ichiro koycmagi Akemi Satoh | 2002 | Jclin lab Anal2002，16，： | 1 |
| 15 | Collagen-Poly(N-Isopropylacrylamide)–Based Membranes for Corneal Stroma Scaffolds显示文摘 | Shigeto Shimmura Charles J. Doillon May Griffith Masatsugu Nakamura Edith Gagnon Akemi Usui Naoshi Shinozaki Kazuo Tsubota | 2003 | Cornea ( Suppl )2003，，7： | 1 |
| 16 | Intra-hepatic arterial administration with miriplatin suspended in an oily lymphographic agent inhibits the growth of tumors implanted in rat livers by inducing platinum-DNA adducts to form and massive apoptosis显示文摘 | Mitsuharu Hanada Akemi Baba Yasuyuki Tsutsumishita Toshihiro Noguchi Takashi Yamaoka Nobuyoshi Chiba Fumio Nishikaku | 2009 | Cancer Chemotherapy and Pharmacology2009，，3： | 1 |
| 17 | Changes in cadmium concen-tration in rice during cooking显示文摘 | Kumiko Shindoh Akemi Yasui | 2003 | Food science and technologyresearch2003，9，2： | 1 |
| 18 | Strategic Payoff from EDI as a Function of EDI Embeddedness显示文摘 | Akemi Takeoka Chatfield Philip Yetton | 2000 | Journal of Management Information Systems2000，，4： | 1 |
| 19 | The role of microRNAs in hepatocarcinogenesis: current knowledge and future prospects显示文摘 | Motoyuki Otsuka Takahiro Kishikawa Takeshi Yoshikawa Motoko Ohno Akemi Takata Chikako Shibata Kazuhiko Koike | 2014 | Journal of Gastroenterology2014，，2： | 1 |
| 20 | Nuclear localization of β-eatenin in the hair matrix cells and differentiated keratinocytes显示文摘 | Hitomi Tsuji Akemi Ishida-Yamamoto Hidetoshi Takahashi | 2001 | Journal of Dermatological Science2001，27，： | 1 |
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