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| 1 | Intravenous administration of glutathione protects parenchymal and non-parenchymal liver cells against reperfusion injury following rat liver transplantation显示文摘AIM:To investigate the effects of intravenous administration of the antioxidant glutathione (GSH) on reperfusion injury following liver transplantation.METHODS:Livers of male Lewis rats were transplanted after 24 h of hypothermic preservation in University of Wisconsin solution in a syngeneic setting. During a 2-h reperfusion period either saline (controls,n=8) or GSH (50 or 100μmol/(h·kg),n=5 each) was continuously administered via the jugular vein.RESULTS:Two hours after starting reperfusion plasma ALT increased to 1 457±281U/L (mean±SE) in controls but to only 908_+187 U/L (P<0.05) in animals treated with 100μmol GSH/(h·kg).No protection was conveyed by 50μmol GSH/(h·kg).Cytoprotection was confirmed by morphological findings on electron microscopy:GSH treatment prevented detachment of sinusoidal endothelial cells (SECs) as well as loss of microvilli and mitochondrial swelling of hepatocytes. Accordingly, postischemic bile flow increased 2-fold. Intravital fluorescence microscopy revealed a nearly complete restoration of sinusoidal blood flow and a significant reduction of leukocyte adherence to sinusoids and postsinusoidal venules. Following infusion of 50μmol and 100 μmol GSH/(h·kg),plasma GSH increased to 65±7mol/L and 97±18μmol/L,but to only 20±3mol/L in untreated recipients.Furthermore, plasma glutathione disulfide (GSSG) increased to 7.5±1.0mol/L in animals treated with 100μmol/(h·kg) GSH but infusion of 50μmol GSH/(h·kg) did not raise levels of untreated controls (1.8±0.5mol/L vs 2.2±0.2mol/L).CONCLUSION:Plasma GSH levels above a critical level may act as a “sink” for ROS produced in the hepatic vasculature during reperfusion of liver grafts.Therefore, GSH can be considered a candidate antioxidant for the prevention of reperfusion injury after liver transplantation, in particular since it has a low toxicity in humans. | RolfJ.Schauer SinanKalmuk Alexander L.Gerbes Rosemarie Leiderer Herbert Meissner Friedrich W.Schildberg Konrad Messmer Manfred Bilzer | 2004 | World Journal of Gastroenterology2004，10，6： | 9 |
| 2 | Highly efficient derivation of ventricular cardiomyocytes from induced pluripotent stem cells with a distinct epigenetic signature显示文摘从 pluripotent 干细胞导出的 Cardiomyocytes 能在药测试,疾病建模和基于房间的治疗被使用。没有 procardiogenic 生长因素,然而,从 pluripotent 干细胞的 cardiomyogenesis 的效率通常是低的,产生 cardiomyocyte 人口是异构的。这里,我们证明导致的 pluripotent 干细胞( iPSCs )能从鼠科的室的 myocytes ( VM )被导出,并且与从各种各样的体的房间类型导出的 iPSCs 的另外的报告一致,自发地作为与遗传上匹配的胚胎的干细胞(转换字符)或 iPSCs 相比区分 cardiomyocytes 进跳动的显著地更高的倾向从尾巴尖端成纤维细胞导出的 导出VM 的 iPSCs ( ViPSCs )展览。惊人地,导出 ViPSC 的 cardiomyocytes 显示的多数室的显型。在 ViPSCs 的提高的室的 myogenesis 在区别的早阶段经由心血管的祖先的增加的数字被调停。以便从 ViPSCs 调查提高的室的 myogenesis 的机制,我们执行了全球基因表示和 DNA methylation 分析,它揭示了可以涉及在 pluripotent 干细胞指定 VM 命运的不同 epigenetic 签名。 | Huansheng Xu B Alexander Yi Hao Wu Christoph Bock Hongcang Gu Kathy O Lui Joo-Hye C Park Ying Shao Alyssa K Riley Ibrahim J Domian Erding Hu Robert Willette John Lepore Alexander Meissner Zhong Wang Kenneth R Chien | 2012 | Cell Research2012，22，1： | 7 |
| 3 | Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA显示文摘 | Luigi Warren Philip D. Manos Tim Ahfeldt Yuin-Han Loh Hu Li Frank Lau Wataru Ebina Pankaj K. Mandal Zachary D. Smith Alexander Meissner George Q. Daley Andrew S. Brack James J. Collins Chad Cowan Thorsten M. Schlaeger Derrick J. Rossi | 2010 | Cell Stem Cell2010，，5： | 4 |
| 4 | PI 3-kinase pathway is responsible for antiapoptotic effects of atrial natriuretic peptide in rat liver transplantation显示文摘AIM:To investigate the in vivo effect of atrial natriureticpeptide(ANP)and its signaling pathway during ortho-topic rat liver transplantation.METHODS:Rats were infused with NaCl,ANP(5 μg/kg),wortmannin(WM,16 μg/kg),or a combination ofboth for 20 min.Livers were stored in UW solution(4°C)for 24 h,transplanted and reperfused.Apoptosis wasexamined by caspase-3 activity and TUNEL staining.Phosphorylation of Akt and Bad was visualized by West-ern blotting and phospho-Akt-localization by confocalmicroscopy.RESULTS:ANP-pretreatment decreased caspase-3activity and TUNEL-positive cells after cold ischemia,indicating antiapoptotic effects of ANP in vivo.The an-tiapoptotic signaling of ANP was most likely caused byphosphorylation of Akt and Bad,since pretreatment withPI 3-kinase inhibitor WM abrogated the ANP-inducedreduction of caspase-3 activity.Interestingly,analysis ofliver tissue by confocal microscopy showed translocationof phosphorylated Akt to the plasma membrane of hepa-tocytes evoked by ANP.CONCLUSION:ANP activates the PI-3-kinase pathwayin the liver in vivo leading to phosphorylation of Bad, an event triggering antiapoptotic signaling cascade inischemic liver. | Uwe Grutzner Melanie Keller Michael Bach Alexandra K Kiemer Herbert Meissner Manfred Bilzer Stefan Zahler Alexander L Gerbes Angelika M Vollmar | 2006 | World Journal of Gastroenterology2006，12，7： | 3 |
| 5 | The Mammalian Epigenome显示文摘 | Bradley E. Bernstein Alexander Meissner Eric S. Lander | 2007 | Cell2007，，4： | 2 |
| 6 | Paternally Induced Transgenerational Environmental Reprogramming of Metabolic Gene Expression in Mammals显示文摘 | Benjamin R. Carone Lucas Fauquier Naomi Habib Jeremy M. Shea Caroline E. Hart Ruowang Li Christoph Bock Chengjian Li Hongcang Gu Phillip D. Zamore Alexander Meissner Zhiping Weng Hans A. Hofmann Nir Friedman Oliver J. Rando | 2010 | Cell2010，，7： | 1 |
| 7 | Proliferation-Dependent Alterations of the DNA Methylation Landscape Underlie Hematopoietic Stem Cell Aging显示文摘 | Isabel Beerman Christoph Bock Brian S. Garrison Zachary D. Smith Hongcang Gu Alexander Meissner Derrick J. Rossi | 2013 | Cell Stem Cell2013，，4： | 1 |
| 8 | 二尖瓣口血流充盈类型对慢性心力衰竭预后判断的价值显示文摘目的 评价超声心动图在临床随访中对慢性心力衰竭 (心衰 )患者预后的判断价值。方法慢性心衰患者 90例 ,平均随访时间为 (18.8± 7.9)月。随访期间 14例死于心衰 ,12例接受心脏移植 ,5例猝死 ,2例死于心肌梗死。依限制性充盈指数 (restrictivefillingindex ,RFI)对患者进行限制性和非限制性充盈的分组。结果 限制性充盈组的心功能更差 ,心源性致死发生率高。根据Kaplan Meier方法 ,则RFI≥ 1的限制性充盈组和RFI <1的非限制性充盈组的 1年和 2年生存率分别为 52 %对 94%和 2 8%对 92 % ,差异有非常显著性意义。Cox多变量模型最终的模型选择RFI为最有效的对慢性心衰发生心源性致死的预后判定的指标 (χ2 =8.80 17;P =0 .0 0 3 0 )。结论 由多普勒参数导出的限制性充盈指数RFI被证实为简单。 | 郑哲岚 Meissner Achim Hausmann Beate Alexander Hermann Simon Ruediger | 2003 | 中华超声影像学杂志2003，12，10： | 1 |
| 9 | Inhalation of Nitric Oxide Prevents Ischemic Brain Damage in Experimental Stroke by Selective Dilatation of Collateral Arterioles显示文摘 | Nicole A. Terpolilli Seong-Woong Kim Serge C. Thal Hiroharu Kataoka Vilia Zeisig Bjoern Nitzsche Benjamin Klaesner Changlian Zhu Susanne Schwarzmaier Lilja Meissner Uta Mamrak Doortje C. Engel Alexander Drzezga Rakesh P. Patel Klas Blomgren Henryk Barthel | 2012 | Circulation Research2012，，5： | 1 |
| 10 | Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA显示文摘 | Luigi Warren Philip D. Manos Tim Ahfeldt Yuin-Han Loh Hu Li Frank Lau Wataru Ebina Pankaj K. Mandal Zachary D. Smith Alexander Meissner George Q. Daley Andrew S. Brack James J. Collins Chad Cowan Thorsten M. Schlaeger Derrick J. Rossi | 2010 | Cell Stem Cell2010，，5： | 1 |
| 11 | The Mammalian Epigenome显示文摘 | Bradley E. Bernstein Alexander Meissner Eric S. Lander | 2007 | Cell2007，，4： | 1 |
| 12 | Reference Maps of Human ES and iPS Cell Variation Enable High-Throughput Characterization of Pluripotent Cell Lines显示文摘 | Christoph Bock Evangelos Kiskinis Griet Verstappen Hongcang Gu Gabriella Boulting Zachary D. Smith Michael Ziller Gist F. Croft Mackenzie W. Amoroso Derek H. Oakley Andreas Gnirke Kevin Eggan Alexander Meissner | 2011 | Cell2011，，3： | 1 |
| 13 | In vitro reprogramming of fibroblasts into a pluripotent ES- cell-like state 显示文摘 | Marius Wernig Alexander Meissner Ruth Foreman | 2007 | Nature2007，448，： | 1 |
| 14 | Proliferation-Dependent Alterations of the DNA Methylation Landscape Underlie Hematopoietic Stem Cell Aging显示文摘 | Isabel Beerman Christoph Bock Brian S. Garrison Zachary D. Smith Hongcang Gu Alexander Meissner Derrick J. Rossi | 2013 | Cell Stem Cell2013，，4： | 1 |
| 15 | Dyes in Carbon Nanotube Arrays显示文摘 | Gerhard Lackner Tobias Mayer-Uhma Ingolf Endler Richard Boucher Frank Meissner Sebastian Scholz Mario Krug Viktor Bezugly Martin Mkandawire Anindya Majumder Daria A. Kovalenko Susan Conze Stefanie Hildebrandt Alexander Michaelis Doru C. Lupascu | 2012 | 材料科学与工程(中英文B版)2012，2，6： | 0 |
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