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| 1 | High-throughput screening of mouse gene knockouts identifies established and novel skeletal phenotypes显示文摘Screening gene function in vivo is a powerful approach to discover novel drug targets. We present high-throughput screening(HTS) data for 3 762 distinct global gene knockout(KO) mouse lines with viable adult homozygous mice generated using either gene-trap or homologous recombination technologies. Bone mass was determined from DEXA scans of male and female mice at 14 weeks of age and by microCT analyses of bones from male mice at 16 weeks of age. Wild-type(WT) cagemates/littermates were examined for each gene KO. Lethality was observed in an additional 850 KO lines. Since primary HTS are susceptible to false positive findings, additional cohorts of mice from KO lines with intriguing HTS bone data were examined. Aging,ovariectomy, histomorphometry and bone strength studies were performed and possible non-skeletal phenotypes were explored. Together, these screens identified multiple genes affecting bone mass: 23 previously reported genes(Calcr, Cebpb, Crtap, Dcstamp, Dkk1, Duoxa2, Enpp1, Fgf23, Kiss1/Kiss1 r, Kl(Klotho),Lrp5, Mstn, Neo1, Npr2, Ostm1, Postn, Sfrp4, Slc30a5, Slc39a13, Sost, Sumf1, Src, Wnt10b), five novel genes extensively characterized(Cldn18, Fam20 c, Lrrk1, Sgpl1, Wnt16), five novel genes with preliminary characterization(Agpat2, Rassf5, Slc10a7, Slc26a7, Slc30a10) and three novel undisclosed genes coding for potential osteoporosis drug targets. | Robert Brommage Jeff Liu Gwenn M Hansen Laura L Kirkpatrick David G Potter Arthur T Ss Brian Zambrowicz David R Powell Peter Vogel | 2014 | Bone Research2014，2，3： | 7 |
| 2 | 卒中介入治疗培训指南:国际多学会共识文件显示文摘1背景
缺血性卒中是全球人口死亡和残疾的首要原因。很多急性大血管闭塞(emergent large vesselocclusion,ELVO)患者都会遗留长期残疾。事实上,这些颅内大动脉闭塞经常会导致大面积脑损伤,进而造成患者死亡或严重致残。 | Lavine SD Cockroft K Hoh B Bambakidis N Khalessi AA Woo H Riina H Siddiqui A Hirsch JA Chong W Rice H Wenderoth J Mitchell P Coulthard A Signh TJ Phatorous C Khangure M Klurfan P ter Brugge K Iancu D Gunnarsson T Pongpech S Rodesch G Soderman M Taylor A Krings T Orbach D Picard L Suh DC Zheng HQ Jansen O Muto M Szikora I Pierot L Brouwer P Gralla J Renowden S Andersson T Fiehler J Turjman F White P Januel AC Spelle L Kulcsar Z Chapot R Biondi A Dima S Taschner C Szajner M Krajina A Sakai N Matsumaru Y Yoshknura S Ezura M Fujinaka T Iihara K Ishii A Higashi T Hirohata M Hyodo A Ito Y Kawanishi M Kiyosue H Kobayashi E Kobayashi S Kuwayama N Matsumoto Y Miyachi S Murayama Y Nagata I Nakahara I Nemoto S Niimi Y Oishi H Satomi J Satow T Sugiu K Tanaka M Terada T Yamagami H Diaz O Lylyk P Jayaraman MV Patsalides A Gandhi CD Lee SK Abruzzo T Albani B Ansari SA Arthur AS Baxter BW Bulsara KR Chen M Almandoz JE Fraser JF Heck DV Hetts SW Hussain MS Klucznik RP Leslie-Mawzi TM Mack WJ McTaggart RA Meyers PM Mocco J Prestigiacomo CA Pride GL Rasmussen PA Starke RM Sunenshine PJ Tarr RW Frei DF Pabo M Nogueira RG Zaidat OO Jovin T Linfante I Yavagal D Liebeskind D Novakovic R Pongpech S 许岩 孙瑞 郭芮兵 | 2017 | 国际脑血管病杂志2017，25，5： | 2 |
| 3 | Desferrioxamine in warm reperfusion media decreases liver injury aggravated by cold storage显示文摘AIM:To evaluate whether desferrioxamine decreases ischemia and perfusion injury aggravated by cold storage(CS)in a rat liver perfusion model. METHODS:Isolated rat livers were kept in CS in University of Wisconsin Solution for 20 h at 4℃,then exposed to 25 min of warm ischemia(WI)at 37℃ followed by 2 h of warm perfusion(WP)at 37℃with oxygenated(95%oxygen and 5%carbon dioxide) Krebs-Henseleit buffer.Desferrioxamine(DFO),an iron chelator,was added at different stages of storage,ischemia and perfusion:in CS only,in WI only,in WP only, in WI and perfusion,or in all stages.Effluent samples were collected after CS and after WI.Perfusate samples and bile were collected every 30 min(0,0.5,1,1.5 and 2 h)during liver perfusion.Cellular injury was assessed by the determination of lactate dehydrogenase(LDH) and aspartate aminotransferase(AST)in the effluent and perfusate samples.Total iron was analysed in the perfusate samples.After WP,the liver was collected for the determination of liver swelling(wet to dry ratio) and liver morphological examination(hematoxylin and eosin staining). RESULTS:Increased CS time caused increased liver dysfunction during WP.After 2 h of WP,liver injury was indicated by increased release of AST(0.5 h CS:9.4± 2.2 U/g liver vs 20 h CS:45.9±10.8 U/g liver,P<0.05) and LDH(0.5 h CS:59±14 U/g liver vs 20 h CS:297 ±71 U/g liver,P<0.05).There was an associated increase in iron release into the perfusate(0.5 h CS:0.11 ±0.03μmoL/g liver vs 20 h CS:0.58±0.10μmoL/g liver,P<0.05)and reduction in bile flow(0.5 h CS: 194±12μL/g vs 20 h CS:71±8μL/g liver,P<0.05). When DFO was added during WI and WP following 20 h of CS,release of iron into the perfusate was de- creased(DFO absent 0.58±0.10μmoL/g liver vs DFO present 0.31±0.06μmoL/g liver,P<0.05),and liver function substantially improved with decreased release of AST(DFO absent 45.9±10.8 U/g liver vs DFO present 8.1±0.9 U/g liver,P<0.05)and LDH(DFO absent 297±71 U/g liver vs DFO present 56±7 U/g liver,P<0.05),and increased bile flow(DFO absent 71±8μL/g liver vs DFO present 237±36μL/g liver, P<0.05).DFO was also shown to improve liver morphology after WP.Cellular injury(the release of LDH and AST)was significantly reduced with the addition of DFO in CS medium but to a lesser extent compared to the addition of DFO in WP or WI and perfusion.There was no effect on liver swelling or bile flow when DFO was only added to the CS medium. CONCLUSION:DFO added during WI and perfusion decreased liver perfusion injury aggravated by extended CS. | Peter G Arthur Xian-Wa Niu Wen-Hua Huang Bastiaan DeBoer Ching Tat Lai Enrico Rossi John Joseph Gary P Jeffrey | 2013 | World Journal of Gastroenterology2013，19，5： | 2 |
| 4 | The functional reserve of corneal endothelium显示文摘 | Shaw E L Rao G N Arthur E J | 1978 | Ophthalmology1978，85，6： | 1 |
| 5 | Selenium in the immune system显示文摘 | ARTHUR J R McKENZIE R C BECKETT G J | 2003 | J Nutr2003，133，5： | 1 |
| 6 | Elevated ambulatory and normal office blood pressure显示文摘 | Goldman Arthur G | 2000 | Annals of Internal Medicine2000，132，10： | 1 |
| 7 | Four new recurring translocations in non-Hodgkin's lymphoma显示文摘 | Levine E G Arthur D C Machnicki J | 1989 | Blood1989，74，5： | 1 |
| 8 | Determination of strains in soils by X-ray method显示文摘 | ROSCOE K H ARTHUR J R F JAMES R G | 1963 | Civil Engineering (London)1963，58，685： | 1 |
| 9 | Determination of stress fields during plane strain of sand mass显示文摘 | ARTHUR J R F JAMES R G ROSCOE K H | 1964 | Geotechnique1964，14，4： | 1 |
| 10 | 5,6-Diaminocytidine,a versatile synthon for pyrimidine-based bicyclic nucleosides显示文摘 | Kallanthottathil G Rajeev Arthur D B | | 0，，23： | 1 |
| 11 | Polyaniline: Synthesis and characterization of pernigraniline base显示文摘 | YAN S MACDIAMID A G ARTHUR J E | 1990 | Jourllal Chemi- cal Society Chemical Communications1990，10，： | 1 |
| 12 | Color segmentation in the HIS color space using the k-means algorithm 显示文摘 | Arthur R Weeks G | 1997 | SPIE1997，9，6： | 1 |
| 13 | Thermal Conductivity Reduction and Thermoelectric Figure of Merit Increase by Embedding Nan- oparticles in Crystalline Semiconductors 显示文摘 | Woochul K Joshua Z Arthur G | 2006 | Physical Review Letters2006，96，04： | 1 |
| 14 | Recombinant mouse OB protein:evidence for a peripheral signal linking adiposity and central neural networks显示文摘 | Arthur L C Francoise J S Yves G | 1995 | Science1995，269，： | 1 |
| 15 | Pyrazole induced oxidative liver injury independent of CYP2E1/2A5 induction due to Nrf2 deficiency显示文摘 | Yongke L U Pengfei G Arthur I C | 2008 | Toxicology2008，，252： | 1 |
| 16 | Hepatic iodo- thyronine 5'-deiodinase: The role of selenium 显示文摘 | ARTHUR J R NICOL F BECKETr G J | 1990 | Bio- chem J1990，272，2： | 1 |
| 17 | Transmission E- lectron Microscopy Characterization of Zircaloy-4 and ZIR- LOTM Oxide Layers显示文摘 | BENOIT de G ARTHUR T M WANG K | 2015 | Journal of Nuclear Materials2015，456，： | 1 |
| 18 | Seleniuminthe immunesystem 显示文摘 | ARTHUR J R MCKENZIE R C BECKETT G J | 2003 | The Journal of Nutrition2003，133，5： | 1 |
| 19 | Effects of gamma radiation on total phenolics,trypsin and tannin inhibitors in soybean grains显示文摘 | De Toledo T C F Canniatti-Brazaca S G Arthur V | 2007 | Radiation Physics and Chemistry2007，76，： | 1 |
| 20 | Changes in cytokine in and auxin concentrations in seaweed concentrates when stored at an elevated temperature显示文摘 | STIRKW A ARTHUR G D | 2004 | J Appl Phycol2004，16，1： | 1 |
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