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| 1 | 应用DiI染色晶体对人胚胎视神经发育过程的初步研究显示文摘目的 应用DiI(1,1′ dioctadecyl 3,3,3′,3′ tetramethylin docarbocyanineperchlorate ,一种羰花青染料 )染色晶体研究人胚胎视网膜、外侧膝状体、上丘及视皮质间连接的形态学特征和动态发育过程。方法 对不同胎龄的 7个胎儿眼球标本进行灌注固定后 ,分别于标本的视束、上丘臂和视皮质下板层植入DiI染色晶体 ,以标记来自视网膜和外侧膝状体的轴突。于室温下放置 4~ 10周 ,等待DiI染色晶体扩散 ,再根据神经走向切片 ,通过激光共焦扫描显微镜观察并记录植入DiI染色晶体 12~ 2 8周的结果。结果 植入DiI染色晶体 12周时 ,胎儿标本的视网膜神经纤维投射已经到达外侧膝状体 ,但尚未出现分层现象 ;视网膜神经投射也已到达上丘 ,纤维位于上丘臂的背侧 ;视皮质下已出现板层结构。植入 2 2周时视皮质下仍存在板层结构。结论 植入DiI染色晶体 12周前 ,视网膜神经纤维投射已到达外侧膝状体和上丘 ,视皮质下的板层也已经形成 ,2 2周后消失。DiI染色技术能有效地用于研究胚胎期视神经的连续发育过程。 | 瞿佳 周翔天 张銮龄 倪海龙 Ken Ashwell 吕帆 | 2002 | 中华眼科杂志2002，38，9： | 5 |
| 2 | Patterns of Teacher Response to Student Writing in a Multiple-Draft Composition Classroom: Is Content Feedback Followed by Form Feedback the Best Method?显示文摘 | Tim Ashwell | 2000 | Journal of Second Language Writing2000，，3： | 4 |
| 3 | Identification of stable normalization genes for quantitative real-time PCR in porcine articular cartilage显示文摘Background: Expression levels for genes of interest must be normalized with an appropriate reference, or housekeeping gene, to make accurate comparisons of quantitative real-time PCR results. The purpose of this study was to identify the most stable housekeeping genes in porcine articular cartilage subjected to a mechanical injury from a panel of 10 candidate genes. Results: Ten candidate housekeeping genes were evaluated in three different treatment groups of mechanically impacted porcine articular cartilage. The genes evaluated were: beta actin, beta-2-microglobulin, glyceraldehyde-3-phosphate dehydrogenase, hydroxymethylbilane synthase, hypoxanthine phosphoribosyl transferase, peptidylprolyl isomerase A (cyclophilin A), ribosomal protein L4, succinate dehydrogenase flavoprotein subunit A, TATA box binding protein, and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein-zeta polypeptide. The stability of the genes was measured using geNorm, BestKeeper, and NormFinder software. The four most stable genes measured via geNorm were (most to least stable) succinate dehydrogenase flavoprotein, subunit A, peptidylprolyl isomerase A, glyceraldehyde-3-phosphate dehydrogenase, beta actin; the four most stable genes measured via BestKeeper were glyceraldehyde-3-phosphate dehydrogenase, peptidylprolyl isomerase A, beta actin, succinate dehydrogenase flavoprotein, subunit A; and the four most stable genes measured via NormFinder were peptidylprolyl isomerase A, succinate dehydrogenase flavoprotein, subunit A, glyceraldehyde-3-phosphate dehydrogenase, beta actin. Conclusions: BestKeeper, geNorm, and NormFinder all generated similar results for the most stable genes in porcine articular cartilage. The use of these appropriate reference genes will facilitate accurate gene expression studies of porcine articular cartilage and suggest appropriate housekeeping genes for articular cartilage studies in other species. | Ryan S McCulloch Melissa S Ashwell Audrey T O'Nan Peter L Mente | 2012 | Journal of Animal Science and Biotechnology2012，3，4： | 2 |
| 4 | 应用神经轴突示踪技术研究人类视觉神经通路胚胎发育显示文摘目的 :应用神经轴突示踪方法 ,研究人类视觉神经通路在胚胎期 12w时的发育情况。方法 :取 12w胎龄的胚胎 ,浸泡在 4 %的甲醛缓冲液中固定后 ,分别在视束、上丘臂和视皮质下植入DiI染料 (DiI,1,1’ dioctadecyl 3,3,3’ ,3’ tetramethylin docarbocyanineperchlorate)。于室温下放置 4w ,待DiI晶体充分扩散后 ,根据神经轴突方向切片 ,通过共焦激光扫描显微镜观察并记录结果。结果 :大体标本观察可见到 ,视网膜神经纤维投射已到达外侧膝状体、上丘。切片观察发现 :外侧膝状体处尚未出现分层现象 ;上丘处 ,来自视网膜的神经纤维位于上丘臂的背侧 ;视皮质下已存在板层结构 ,板层内神经元呈多种形态。结论 :通过神经轴突示踪技术发现 ,12w时视网膜的神经纤维投射已经到达外侧膝状体和上丘 ,视皮质下存在板层结构。这说明人类视觉神经通路发育存在“等待期”。 | 周翔天 程刚 吕帆 张銮龄 倪海龙 Ken Ashwell 瞿佳 | 2004 | 眼视光学杂志2004，6，2： | 2 |
| 5 | Is There a Zone of Vascular Vulnerability in the Fetal Brain Stem?显示文摘 | Sharon Leong Ken W.S Ashwell | 1997 | Neurotoxicology and Teratology1997，，： | 2 |
| 6 | Molecular genotype identification of the Gallus gallus major histocompatibility complex显示文摘 | Fulton J E Juul-Madsen H R Ashwell C M | | 0，，5： | 1 |
| 7 | Advances in secondary spinal cord injury : role of apoptosis 显示文摘 | LU J ASHWELL K W WAITE P | 2000 | Spine2000，25，14： | 1 |
| 8 | Olfactory ensheathing cells显示文摘 | Lu J Ashwell K | 2002 | Spine2002，27，： | 1 |
| 9 | Detection of putative loci affecting milk,health,and conformation traits in a US Holstein population using 105 microsatellite markers显示文摘 | Van Tassell C P Ashwell M S Sonstegard T S | 2000 | J Dairy Sci2000，83，8： | 1 |
| 10 | Waist-to-height ratio is a better screening tool than waist circumference and BMI for adult cardio- metabolic risk factors : systematic review and meta-analysis 显示文摘 | Ashwell M Gunn P Gibson S | 2012 | Obesi Rev2012，13，3： | 1 |
| 11 | Six reasons why the waist-to-height ratio is a rapid and effective global indicator for health risks of obesity and how its use could simplify the international public health message on obesity显示文摘 | Ashwell M Hsieh S D | 2005 | lnt J Food Sci Nutr2005，56，5： | 1 |
| 12 | Detection of loci affecting milk production and health traits in an elite US Holstein population using microsatellite markers显示文摘 | Ashwell M S Rexroad Jr C E Miller R H | 1997 | Animal Genetics1997，28，3： | 1 |
| 13 | Six reasons why the waist - to - height ratio is a rapid and effective global indicator for health risks of obesity and how its use could simplify the international public health message on obesity 显示文摘 | Ashwell M Hsieh SD | 2005 | Int J Food Sci Nutr2005，56，5： | 1 |
| 14 | Advances in secondary spinalcord injury:role of apoptosis显示文摘 | Lu J Ashwell K W Waite P | 2000 | Spine2000，25，14： | 1 |
| 15 | Patterns of Teacher Response to Student Writing in a Multiple-Draft Composition Classroom: Is Content Feedback Followed by Form Feedback the Best Method?显示文摘 | Tim Ashwell | 2000 | Journal of Second Language Writing2000，，3： | 1 |
| 16 | Comparative map alignment of BTA27 and HSA4 and 8to identify conserved segments of genome containing fat deposition QTL 显示文摘 | Wes M Garrett Melissa S Ashwell | 2000 | Mamm Genome2000，11，： | 1 |
| 17 | Waist to height ratio is a simple and effective obesity screening tool for cardiovascular risk factors:analysis of data from the British National Diet and Nutrition Survey of adults aged 19-64 years显示文摘 | Ashwell M Gibson S | 2009 | Obes Facts2009，2，2： | 1 |
| 18 | Advances in secondary spinal cord injury : role of apoptosis显示文摘 | Lu J Ashwell K W Waite P | 2000 | Spine2000，25，14： | 1 |
| 19 | Olfactory ensheathing cells ( Their Potential Use for Repairing the Injured Spinal Cord) 显示文摘 | Jike L Ashwell K | 2002 | Spine2002，27，： | 1 |
| 20 | GAP-43 Immunoreactivity in the brain of the developing and adult wallaby(Macropus eugenii)显示文摘 | Hassiotis M Ashwell KW Marotte LR | 2002 | Anat Embryol2002，206，： | 1 |
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