|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Autophagy inhibition by chloroquine sensitizes HT-29 colorectal cancer cells to concurrent chemoradiation显示文摘AIM:To investigate whether the inhibition of autophagy by chloroquine(CQ)sensitizes rectal tumors to radiation therapy(RT)or concurrent chemoradiation(chemoRT).METHODS:In vitro,HCT-116 and HT-29 colorectal cancer(CRC)cell lines were treated as following:(1)PBS;(2)CQ;(3)5-fluorouracil(5-FU);(4)RT;(5)CQ and RT;(6)5-FU and RT;(7)CQ and 5-FU;and(8)5-FU and CQ and RT.Each group was then exposed to various doses of radiation(0-8 Gy)depending on the experiment.Cell viability and proliferative capacity were measured by3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)and clonogenic assays.Clonogenic survivalcurves were constructed and compared across treatment groups.Autophagy status was determined by assessing the LC3-Ⅱto LC3-Ⅰratio on western blot analysis,autophagosome formation on electron microscopy and identification of a perinuclear punctate pattern with GFPlabeled LC3 on fluorescence microscopy.Cell cycle arrest and cell death were evaluated by FACS and AnnexinⅤanalysis.All experiments were performed in triplicate and statistical analysis was performed by the student’s t test to compare means between treatment groups.RESULTS:RT(2-8 Gy)induced autophagy in HCT-116and HT-29 CRC cell lines at 4 and 6 h post-radiation,respectively,as measured by increasing LC3-Ⅱto LC3-Ⅰratio on western blot.Additionally,electron microscopy demonstrated autophagy induction in HT-29 cells24 h following irradiation at a dose of 8 Gy.Drug treatment with 5-FU(25μmol/L)induced autophagy and the combination of 5-FU and RT demonstrated synergism in autophagy induction.CQ(10μmol/L)alone and in combination with RT effectively inhibited autophagy and sensitized both HCT-116 and HT-29 cells to treatment with radiation(8 Gy;P<0.001 and 0.00001,respectively).Significant decrease in clonogenic survival was seen only in the HT-29 cell line,when CQ was combined with RT at doses of 2 and 8 Gy(P<0.5 and P=0.05,respectively).There were no differences in cell cycle progression or Annexin V staining upon CQ addition to RT.CONCLUSION:Autophagy inhibition by CQ increases CRC cell sensitivity to concurrent treatment with 5-FU and RT in vitro,suggesting that addition of CQ to chemoRT improves CRC treatment response. | Caitlin A Schonewolf Monal Mehta Devora Schiff Hao Wu Bruce G Haffty Vassiliki Karantza Salma K Jabbour | 2014 | World Journal of Gastrointestinal Oncology2014，6，3： | 12 |
| 2 | Patterns of in-hospital mortality and bleeding complications following PCI for very elderly patients: insights from the Dartmouth Dynamic Registry显示文摘BackgroundVery 老病人(年龄 85 年) 是人口的一个很快增加的片断。作为一个组,他们经历跟随经皮的冠的干预(一种总线标准) 的在里面医院死亡和流血复杂并发症的高率。然而,在流血和死亡在之间的关系老 unknown.MethodsRetrospective 评论在 Dartmouth-Hitchcock 从 2000 ~ 2015 在 17,378 连续一种总线标准过程上被执行医学中心。在索引一种总线标准承认期间流血的发生(流血要求的输送,存取地点 hematoma > 5 厘米, pseudoaneurysm,和 retroperitoneal 流血)? 并且在里面医院死亡为四个年龄组被报导(< 65 年, 65-74 年, 75-84 年,和 85 年) 。承受了谁的流血复杂并发症和那些的病人的死亡被计算, multivariate 分析为在里面医院被执行死亡。最后,知道流血的预言者在病人年龄之间被比较 < 85 年和年龄 17,378 个病人学习了的 85 years.ResultsOf,(5.9%) 1019 经历了流血,(2.1%) 369 死了在里面医院追随者一种总线标准。流血和在里面医院死亡的发生与增加年龄 monotonically 增加了(死亡:0.94% , 2.27% , 4.24% 和 4.58% ;流血:3.96% , 6.62% , 10.68% 和 13.99% 好久 < 65, 65-74, 75-84 和 85 年,分别地) 。除了病人年龄 85 年,在 multivariate 分析上,流血为所有年龄组与增加的死亡被联系[机会比率(95% CI ) :变老 < 65 年, 3.65 (1.99-6.74 ) ;年龄 65-74 年, 2.83 (1.62-4.94 ) ;年龄 75-84 年, 3.86 (2.56-5.82 ) ,年龄 85 年:1.39 (0.49-3.95 )] 有增加的 .ConclusionsBleeding 和死亡追随者一种总线标准增加变老。为老尽管有流血的高率,流血在里面医院死亡追随者一种总线标准不再是预兆的。 | Shawn X Li Hannah I Chaudry Jiyong Lee Theodore B Curran Vishesh Kumar Kendrew K Wong Bruce W Andrus James T DeVries | 2018 | Journal of Geriatric Cardiology2018，15，2： | 6 |
| 3 | 循证医学:做好研究的基础显示文摘写出优秀的论文很重要,但更重要的是首先要有好数据。然而,调查研究若没有计划或者开展好,便不会有好的数据。所以写出好文章的关键是在研究前进行详尽完备的研究设计。因此.本文着重讲述合理的设计实验并写出优秀的论文。 | Bruce K Rubin 周正霄(译) 许诺(校) 白春学(校) | 2009 | 国际呼吸杂志2009，29，1： | 2 |
| 4 | Laser patterning for the study of MSC cardiogenic differentiation at the single-cell level显示文摘Mesenchymal stem cells(MSCs)have been cited as contributors to heart repair through cardiogenic differentiation and multiple cellular interactions,including the paracrine effect,cell fusion,and mechanical and electrical couplings.Due to heart–muscle complexity,progress in the development of knowledge concerning the role of MSCs in cardiac repair is heavily based on MSC–cardiomyocyte coculture.In conventional coculture systems,however,the in vivo cardiac muscle structure,in which rod-shaped cells are connected end-to-end,is not sustained;instead,irregularly shaped cells spread randomly,resulting in randomly distributed cell junctions.Consequently,contact-mediated cell–cell interactions(e.g.,the electrical triggering signal and the mechanical contraction wave that propagate through MSC–cardiomyocyte junctions)occur randomly.Thus,the data generated on the beneficial effects of MSCs may be irrelevant to in vivo biological processes.In this study,we explored whether cardiomyocyte alignment,the most important phenotype,is relevant to stem cell cardiogenic differentiation.Here,we report(i)the construction of a laser-patterned,biochip-based,stem cell–cardiomyocyte coculture model with controlled cell alignment;and(ii)single-cell-level data on stem cell cardiogenic differentiation under in vivo-like cardiomyocyte alignment conditions. | Zhen Ma Qiuying Liu Huaxiao Yang Raymond B Runyan Carol A Eisenberg Meifeng Xu Thomas K Borg Roger Markwald Yifei Wang Bruce Z Gao | 2013 | Light(Science & Applications)2013，2，1： | 2 |
| 5 | Hierarchical adaptive dynamic power management 显示文摘 | Ren Zhiyuan BRUCE H K RADU M | 2005 | IEEE Transactions on Computers2005，54，4： | 1 |
| 6 | Customer Loyalty Isn't Enough Grow Your Share of Wallet显示文摘 | Timothy L K Lerzan A Alexander Buoye Bruce Cooii | 2011 | Harvard Business Review2011，，10： | 1 |
| 7 | Diet-induced maternalobesity alters ovarian morphology and gene expression in the adultmouse offspring显示文摘 | Cheong Y Sadek K H Bruce K D | 2014 | Fertil Steril2014，102，3： | 1 |
| 8 | Fractures of the calcaneus显示文摘 | DAVID P BRUCE J STEPHEN K | 2002 | Orthop Clin North(Am)2002，33，1： | 1 |
| 9 | Perioperative pharmacology: a focus on aminoglycosides 显示文摘 | Bruce K Hicks RW | 2011 | Aorn J2011，93，4： | 1 |
| 10 | Thermophilic aerobic digestionA reliable and effective process for sludge treatment at sm&ll works显示文摘 | Murray K C Tong A Bruce A M | 1990 | Water Sci Technol1990，22，34： | 1 |
| 11 | The structure of self-consciousness in children and young adolescents and relations to social anxiety显示文摘 | Charmaine K Higa Lisa K Phillips Bruce F Chorpita | 2008 | Journal of Psychopathology and Behavioral Assessment2008，30，4： | 1 |
| 12 | Engineering and kinetic characterisation of two glucosy|transferases from Arabidopsis thaliana 显示文摘 | Weis M Lim E K Bruce N C | 2008 | Biochimie2008，90，： | 1 |
| 13 | Psychological disorders in patients with evacuation disorders and constipation in a tertiary practice显示文摘 | Nehra V Bruce B K Rath-Harvey D M | 2000 | Am J Gastroenterol2000，95，7： | 1 |
| 14 | A first linkage map of pecan cultivars based on RAPD and AFLP markers显示文摘 | SUDHEER R SUE K BRUCE W | 2005 | Theor Appl Genet2005，110，： | 1 |
| 15 | Formation of chlorinated organics during solid waste combustion显示文摘 | Gullett B K Bruce K R Beach L O | 1990 | Waste Management and Research1990，8，： | 1 |
| 16 | Selection and characterization of β-lactam-β-lactamase inactivator-resistant mutants following PCR mutagenesis of the TEM-1 β-lactamase gene显示文摘 | Sergei B V Bruce G Lakshmi P K | 1998 | Antimicrob Agents Chemother1998，42，： | 1 |
| 17 | Respiratory Care and Cystic Fibrosis显示文摘 | David E Geller MD Bruce K | 2009 | Respir Care2009，54，6： | 1 |
| 18 | 显示文摘 | Shaju K M Bruce P G | 2006 | Adv Mater2006，18，17： | 1 |
| 19 | Validation of analytical methods used incleaning validation显示文摘 | Herbert J K Bruce R | 2004 | J Validat Technol2004，10，3： | 1 |
| 20 | Purification and primary structural charaeterization of prophenoloxidases from Aedes aegypti larvae显示文摘 | Junsuo S L Seong R K Bruce M C | 2005 | Insect Biochem Mol Biol2005，35，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员