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您的检索式：作者名="Bruno G Defilippi"
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| 1 | A dual positional specific lipoxygenase functions in the generation of flavor compounds during climacteric ripening of apple显示文摘Lipoxygenase(LOX)is an important contributor to the formation of aroma-active C6 aldehydes in apple(Malus×domestica)fruit upon tissue disruption but little is known about its role in autonomously produced aroma volatiles from intact tissue.We explored the expression of 22 putative LOX genes in apple throughout ripening,but only six LOXs were expressed in a ripening-dependent manner.Recombinant LOX1:Md:1a,LOX1:Md:1c,LOX2:Md:2a and LOX2:Md:2b proteins showed 13/9-LOX,9-LOX,13/9-LOX and 13-LOX activity with linoleic acid,respectively.While products of LOX1:Md:1c and LOX2:Md:2b were S-configured,LOX1:Md:1a and LOX2:Md:2a formed 13(R)-hydroperoxides as major products.Site-directed mutagenesis of Gly567 to an alanine converted the dual positional specific LOX1:Md:1a to an enzyme with a high specificity for 9(S)-hydroperoxide formation.The high expression level of the corresponding MdLOX1a gene in stored apple fruit,the genetic association with a quantitative trait locus for fruit ester and the remarkable agreement in regio-and stereoselectivity of the LOX1:Md:1a reaction with the overall LOX activity found in mature apple fruits,suggest a major physiological function of LOX1:Md:1a during climacteric ripening of apples.While LOX1:Md:1c,LOX2:Md:2a and LOX2:Md:2b may contribute to aldehyde production in immature fruit upon cell disruption our results furnish additional evidence that LOX1:Md:1a probably regulates the availability of precursors for ester production in intact fruit tissue. | Doreen Schiller Carolina Contreras Jörg Vogt Frank Dunemann Bruno G Defilippi Randolph Beaudry Wilfried Schwab | 2015 | Horticulture Research2015，2，1： | 6 |
| 2 | Apple aroma:alcohol acyltransferase, a rate limiting step for ester biosynthesis, is regulated by ethylene 显示文摘 | Defilippi Bruno G Kader Adel A Dandekar Abhaya M | 2005 | Plant Science2005，168，： | 1 |
| 3 | Apple aroma:alcohol acyhransferase, a rate limiting step for ester biosynthesis, is regulated by ethylene 显示文摘 | Defilippi Bruno G Kader Adel A Dandekar Abhaya M | 2005 | Plant Science2005，168，： | 1 |
| 4 | Apple aroma: alcohol acyltransferase, a rate limiting step for ester biosynthesis is regulated by ethylene显示文摘 | Bruno G Defilippi Adel A | 2005 | Plant Science2005，168，： | 1 |
| 5 | High-CO2 controlled atmospheres reduce decay incidence in Thompson seedless and red globe table grapes显示文摘 | Julio Retamales Bruno G Defilippi | 2003 | Postharvest Biology and Technology2003，29，： | 1 |
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