|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Liver fat deposition and mitochondrial dysfunction in morbid obesity:An approach combining metabolomics with liver imaging and histology显示文摘AIM: To explore the usefulness of magnetic resonance imaging(MRI) and spectroscopy(MRS) for assessment of non-alcoholic fat liver disease(NAFLD) as compared with liver histological and metabolomics findings. METHODS: Patients undergoing bariatric surgery following procedures involved in laparoscopic sleeve gastrectomy were recruited as a model of obesityinduced NAFLD in an observational, prospective, singlesite, cross-sectional study with a pre-set duration of 1 year. Relevant data were obtained prospectively and surrogates for inflammation, oxidative stress and lipid and glucose metabolism were obtained through standard laboratory measurements. To provide reliable data from MRI and MRS, novel procedures were designed to limit sampling variability and other sources of error using a 1.5T Signa HDx scanner and protocols acquired from the 3D or 2D Fat SAT FIESTA prescription manager. We used our previously described 1H NMRbased metabolomics assays. Data were obtained immediately before surgery and after a 12-mo period including histology of the liver and measurement of metabolites. Values from 1H NMR spectra obtained after surgery were omitted due to technical limitations.RESULTS: MRI data showed excellent correlation with the concentration of liver triglycerides, other hepatic lipid components and the histological assessment, w h i c h e xc l u d e d t h e p r e s e n c e o f n o n-a l c o h o l i c steatohepatitis(NASH). MRI was sufficient to follow up NAFLD in obese patients undergoing bariatric surgery and data suggest usefulness in other clinical situations. The information provided by MRS replicated that obtained by MRI using the-CH3 peak(0.9 ppm), the-CH2- peak(1.3 ppm, mostly triglyceride) and the-CH=CH- peak(2.2 ppm). No patient depicted NASH. After surgery all patients significantly decreased their body weight and steatosis was virtually absent even in patients with previous severe disease. Improvement was also observed in the serum concentrations of selected variables. The most relevant findings using metabolomics indicate increased levels of triglyceride and monounsaturated fatty acids in severe steatosis but those results were accompanied by a significant depletion of diglycerides, polyunsaturated fatty acids, glucose-6-phosphate and the ATP/AMP ratio. Combined data indicated the coordinated action on mitochondrial fat oxidation and glucose transport activity and may support the consideration of NAFLD as a likely mitochondrial disease. This concept may helpto explain the dissociation between excess lipid storage in adipose tissue and NAFLD and may direct the search for plasma biomarkers and novel therapeutic strategies. A limitation of our study is that data were obtained in a relatively low number of patients.CONCLUSION: MRI is sufficient to stage NAFLD in obese patients and to assess the improvement after bariatric surgery. Other data were superfluous for this purpose. | Nahum Calvo Raúl Beltrán-Debón Esther Rodríguez-Gallego Anna Hernández-Aguilera Maria Guirro Roger Mariné-Casadó Lidón Millá Josep M Alegret Fàtima Sabench Daniel del Castillo María Vinaixa Miguelàngel Rodríguez Xavier Correig Roberto García-álvarez Javier A Menendez Jordi Camps Jorge Joven | 2015 | World Journal of Gastroenterology2015，21，24： | 2 |
| 2 | Elimination of organic water pollutants using adsorbents obtained from sewage sludge 显示文摘 | Otero M Rozada F Calvo L F | 2003 | Dyes and Pigments2003，57，： | 1 |
| 3 | Detection of new respiratory viruses in hospitalized infants with bronchiolitis: a three-year r prospective study显示文摘 | Calvo C Pozo F Garcia-Garcia M L | 2010 | Acta Paediatrica2010，99，6： | 1 |
| 4 | Analysis of the cocombustion of sewage sludge and coal by TG-MS显示文摘 | Orero M Diez C Calvo L F | 2002 | Biomass&Bioenergy2002，22，4： | 1 |
| 5 | Kinetics and equilibrium modeling of the methylene blue removal from solution by adsorbent materials produced from sewage sludges 显示文摘 | Otero M Rozada F Calvo L F | 2003 | Biochemical Engineering Journal2003，15，1： | 1 |
| 6 | Analysis of the coeom- bustion of sewage sludge and coal by TG-MS显示文摘 | Otero M Dlez C Calvo L F | 2002 | Bio- mass and Bioenergy2002，22，4： | 1 |
| 7 | Kinetic and equilibrium modelling of the methylene blue removal from solution by adsorbent materials produced from sewage sludges显示文摘 | Otero M Rozada F Calvo L F | 2003 | Biochemical Engineering Journal2003，15，： | 1 |
| 8 | Surfactant activity of a naphthalene degrading Bacillus pumilus strain isolated from oil sludge显示文摘 | Calvo C Toledo F L Gonzalez-lopez J | 2004 | Journal of Biotechnology2004，109，： | 1 |
| 9 | Elimination of organic water pollutants using adsorbents obtained from sewage sludge 显示文摘 | M Otero F Rozada L F Calvo | 2003 | Dye and Pigments2003，57，4： | 1 |
| 10 | Elimination of organic water pollutants using adsorbents obtained from sewage sludge显示文摘 | Otero M Rozada F Calvo L F | 2003 | Dyes and Pigments2003，，57： | 1 |
| 11 | Surfactant activity of a naphthalene degrading Bacillus pumilus strain isolated from oil sludge显示文摘 | Calvo C Toledo F L Gonzalez-lopez J | 2004 | Journal of Biotechnology2004，109，： | 1 |
| 12 | Elimination of organic waterpollutants using adsorbents obtained from sewage sludge 显示文摘 | Otero M Rozada F Calvo L F l | 2003 | Dyes and Pigments2003，57，1： | 1 |
| 13 | Sulphur partitioning in sediments and biodeposits below mussel rafts in the Ria de Arousa (Galicia, NW Spain) 显示文摘 | Otero X L Calvo De Anta R M Macias F | 2006 | Marine Environmental Re- search2006，61，： | 1 |
| 14 | Dye adsorption by sewage sludge- based activated carbons in batch and fixed- bed systems 显示文摘 | Rozada F Calvo L F Garcia A I | 2003 | Bioresource Technology2003，87，3： | 1 |
| 15 | Clinical proteomics:from biomarker discovery and cell signaling profiles to individualized personal therapy显示文摘 | Calvo K R Liotta L A Petricoin E F | 2005 | Biosci Rep2005，25，12： | 1 |
| 16 | Clinical proteomics: from biomarker discovery and cell signaling profiles to individualized personal therapy 显示文摘 | Calvo K R Liotta L A Petricoin E F | 2005 | Biosci Rep2005，25，12： | 1 |
| 17 | Elimination of organic water pollutants using adsorbents obtained from sewage sludge显示文摘 | Otero M Rozada F Calvo L F | 2003 | Dyes and Pigments2003，57，： | 1 |
| 18 | Kinetic and equilibrium modelling of the methylene blue removal from solution by adsorbent materials produced from sewage sludges显示文摘 | OTERO M ROZADA F CALVO L F | 2003 | Biochemical Engineering Journal2003，15，1： | 1 |
| 19 | Recovery of germination from different osmotic conditions by four halophytes from south-eastern Spain显示文摘 | Pujol J A Calvo J F RamirezDiaz L | 2000 | Ann Bot2000，85，： | 1 |
| 20 | thermogravimetry as a technique for establishing the stabilization progress of sludge from wastewater treatment plants显示文摘 | Otero M Calvo L F Estrada B | 2001 | TheemochimicaActa2001，389，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员