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| 1 | Enhanced secondary pollution offset reduction of primary emissions during COVID-19 lockdown in China显示文摘To control the spread of the 2019 novel coronavirus(COVID-19),China imposed nationwide restrictions on the movement of its population(lockdown)after the Chinese New Year of 2020,leading to large reductions in economic activities and associated emissions.Despite such large decreases in primary pollution,there were nonetheless several periods of heavy haze pollution in eastern China,raising questions about the well-established relationship between human activities and air quality.Here,using comprehensive measurements and modeling,we show that the haze during the COVID lockdown was driven by enhancements of secondary pollution.In particular,large decreases in NOx emissions from transportation increased ozone and nighttime NO_(3)radical formation,and these increases in atmospheric oxidizing capacity in turn facilitated the formation of secondary particulate matter.Our results,afforded by the tragic natural experiment of the COVID-19 pandemic,indicate that haze mitigation depends upon a coordinated and balanced strategy for controlling multiple pollutants. | Xin Huang Aijun Ding Jian Gao Bo Zheng Derong Zhou Ximeng Qi Rong Tang Jiaping Wang Chuanhua Ren Wei Nie Xuguang Chi Zheng Xu Liangduo Chen Yuanyuan Li Fei Che Nini Pang Haikun Wang Dan Tong Wei Qin Wei Cheng Weijing Liu Qingyan Fu Baoxian Liu Fahe Chai Steven JDavis Qiang Zhang Kebin He | 2021 | National Science Review2021，8，2： | 47 |
| 2 | Microstructure and Mechanical Properties of C/C-ZrC-SiC Composites Fabricated by Reactive Melt Infiltration with Zr,Si Mixed Powders显示文摘To meet the increasing demand for advanced materials capable of operation over 2000℃for future thermal protection systems application,C/C—ZrC—SiC composites were fabricated by reactive melt infiltration(RMI) with Zr,Si mixed powders as raw materials.The structural evolution and formation mechanism of the C/C—ZrC -SiC composites were discussed,and the mechanical property of the as-prepared material was investigated by compression test.The results showed that after the RMI process,a special structure with ZrC-SiC multi-coating as outer layer and ZrC-SiC-PyC ceramics as inner matrix was formed.ZrC and SiC rich areas were formed in the composites and on the coating surface due to the formation of Zr-Si intermetallic compounds in the RMI process.Mechanical tests showed that the average compression strength of the C/C-ZrC-SiC composites was 133.86 MPa,and the carbon fibers in the composites were not seriously damaged after the RMI process. | Xin Yang Zhean Su Qizhong Huang Xiao Fang Liyuan Chai | 2013 | Journal of Materials Science & Technology2013，29，8： | 25 |
| 3 | miR-20a targets BNIP2 and contributes chemotherapeutic resistance in colorectal adenocarcinoma SW480 and SW620 cell lines显示文摘化疗是为 colorectal 腺癌癌症的重要治疗;然而, colorectal 腺癌房间经常开发抵抗到化学疗法的药,导致恶化和差的耐心的预后。药抵抗的发展经常是一个 multifactor 过程,它包含了几基因和细胞的机制。microRNAs 是否定地在 post-transcriptional 水平调整基因表示的内长的小非编码的 RNA。在现在的学习,我们在调整 colorectal 腺癌房间 SW620 和 SW480 的药敏感调查了 microRNAs 的可能的角色。使用 microRNA 表达式数组和我们发现了房间展出了的那 SW620 的量的反向的 transcriptase (RT )-PCR, 与 SW480 房间相比提高了 miR-20a 表示。另外,这二房间行显示了不同敏感到化学疗法的药氟尿嘧啶, oxaliplatin,和 teniposide。miR-20a 的调整改变了 SW620 和 SW480 房间的敏感到这些药;miR-20a 击倒敏化的 SW620 房间到化学疗法的代理人,而在 SW480 房间的 miR-20a 的 overexpression 导致了 chemoresistance。内长的 BNIP2 mRNA 和 BNIP2 蛋白质层次是相反地由量的 RT-PCR 和西方的污点分析与是的 miR-20a 层次有关检测了。荧光记者试金显示出在 miR-20a 和 BNIP2 3UTR 之间的一个直接相互作用。一起拿,我们的调查结果建议 miR-20a 可以在 colorectal 腺癌癌症房间药抵抗起一个作用并且可以是对在 colorectal 腺癌的化疗药抵抗的一个治疗学的目标。 | Huijuan Chai Min Liu Ruiqing Tian Xin Li Hua Tang | 2011 | Acta Biochimica et Biophysica Sinica2011，43，3： | 18 |
| 4 | Metabolic engineering and flux analysis of Corynebacterium glutamicum for L-serine production显示文摘L-Serine plays a critical role as a building block for cell growth, and thus it is difficult to achieve the direct fermentation of L-serine from glucose. In this study, Corynebacterium glutamicum ATCC 13032 was engineered de novo by blocking and attenuating the conversion of L-serine to pyruvate and glycine, releasing the feedback inhibition by L-serine to 3-phosphoglycerate dehydrogenase (PGDH), in combination with the co-expression of 3-phosphoglycerate kinase (PGK) and feedback-resistant PGDH (PGDHr). The resulting strain, SER-8, exhibited a lower specific growth rate and significant differences in L-serine levels from Phase I to Phase V as determined for fed-batch fermentation. The intracellular L-serine pool reached (14.22±1.41) μmol gCDM 1, which was higher than glycine pool, contrary to fermentation with the wild-type strain. Furthermore, metabolic flux analysis demonstrated that the over-expression of PGK directed the flux of the pentose phosphate pathway (PPP) towards the glycolysis pathway (EMP), and the expression of PGDHr improved the L-serine biosynthesis pathway. In addition, the flux from L-serine to glycine dropped by 24%, indicating that the deletion of the activator GlyR resulted in down-regulation of serine hydroxymethyltransferase (SHMT) expression. Taken together, our findings imply that L-serine pool management is fundamental for sustaining the viability of C. glutamicum, and improvement of C1 units generation by introducing the glycine cleavage system (GCV) to degrade the excessive glycine is a promising target for L-serine production in C. glutamicum. | LAI ShuJuan ZHANG Yun LIU ShuWen LIANG Yong SHANG XiuLing CHAI Xin WEN TingYi | 2012 | Science China(Life Sciences)2012，55，4： | 15 |
| 5 | Clean Energy Consumption of Power Systems Towards Smart Agriculture: Roadmap, Bottlenecks and Technologies显示文摘Over the past decades,both agriculture and power systems have faced serious problems,such as the power supply shortage in agriculture,and difficulties of clean energy consump-tion in the power system.To address and overcome these issues,this paper proposes an idea to combine smart agriculture and clean energy consumption,use surplus clean energy to supply agriculture production,and utilize smart agriculture to support power system with clean energy penetration.A comprehensive review has been conducted to first depict the roadmap of coupling a agriculture-clean energy system,analyze their feasibilities and advantages.The recent technologies and bottlenecks are summa-rized and evaluated for the development of a combined system consisting of smart agriculture production and clean energy consumption.Several case studies are introduced to explore the mutual benefits of agriculture-clean energy systems in both the energy and food industries. | Junyong Liu Yanxin Chai Yue Xiang Xin Zhang Si Gou Youbo Liu | 2018 | CSEE Journal of Power and Energy Systems2018，4，3： | 9 |
| 6 | A distinct pattern of memory and attention deficiency in patients with depression显示文摘 | LUO Lan-lan CHEN Xin CHAI Yan LI Jin-hong HANG Mian ZHANG Jian-ning | 2013 | Chinese Medical Journal2013，，6： | 9 |
| 7 | Determination of rare earth elements in plant protoplasts by MAA显示文摘A preliminary study on the speciation of rare earth elements in plant cells has been carried out by molecular activation analysis (MAA). Mesophyll protoplasts of Brassica napus were isolated by enzymatic digestion. After being washed with isosmotic solution containing EDTA for several times, the protoplasts were purified by gradient centrifugation. Then the concentration of rare earth elements (REEs) in the protoplasts was determined by neutron activation analysis. The result shows that REEs can enter the cells of the plant. | ZHANG Zhiyong WANG Yuqi SUN Jingxin LI Fuliang CHAI Zhifang XU Lei LI Xin CAO Guoyin | 2000 | Chinese Science Bulletin2000，45，16： | 8 |
| 8 | Effects of glucocorticoids on traumatic brain injury related critical illness-related corticosteroid insufficiency显示文摘 | ZHAO Zi-long CHEN Xin ZHU Hui ZHANG Bao-liang CHAI Yan LI Xin-yuan DONG Jing-fei ZHANG Jian-ning | 2013 | Chinese Medical Journal2013，，19： | 8 |
| 9 | Texture control by {10-12} twinning to improve the formability of Mg alloys: A review显示文摘Texture control of wrought Mg alloys, particularly in rolled Mg alloy sheets, has been an important research topic for the past several decades because it has significant influence on stretch formability at room temperature. For Mg alloys, {10-12} twinning can be easily activated and causes a 86.3° lattice rotation. Thus, pre-twinning deformation is considered as an effective and low-cost method for texture control in wrought Mg and its alloys. Furthermore, it has been verified that texture control via pre-twinning deformation can remarkably improve stretch formability of rolled Mg alloy sheets. In this review, recent researches on texture control via twinning deformation and its influence on stretch formability will be critically reviewed. The main contents include the micro-mechanism and impact factors of control in twin-orientation, plastic processing techniques of pre-inducing twins and the application of pre-induced twins in improvement of stretch formability. Finally, further research directions on this field were proposed. | Bo Song Qingshan Yang Tao Zhou Linjiang Chai Ning Guo Tingting Liu Shengfeng Guo Renlong Xin | 2019 | Journal of Materials Science & Technology2019，35，10： | 8 |
| 10 | A natural variation in Ribonuclease H-like gene underlies Rht8 to confer“Green Revolution”trait in wheat显示文摘Dear Editor,Introduction of gibberellin(GA)-insensitive Reduced height(Rht)genes,Rht-B1b and Rht-D1b,has resulted in the“Green Revolution”in modern wheat cultivars(Triticum aestivum)that has skyrocketed wheat grain yields worldwide since the 1960s(Peng et al.,1999;Velde et al.,2021).However,Rht-B1b/D1b also reduce coleoptiles,which is undesired in dryland regions where deep planting is essential for seedling establishment(Rebetzke et al.,1999,Rebetzke et al.,2001;Ellis et al.,2004). | Lingling Chai Mingming Xin Chaoqun Dong Zhaoyan Chen Huijie Zhai Junhong Zhuang Xuejiao Cheng Naijiao Wang Jia Geng Xiaobo Wang Ruolin Bian Yingyin Yao Weilong Guo Zhaorong Hu Huiru Peng Guihua Bai Qixin Sun Zhenqi Su Jie Liu Zhongfu Ni | 2022 | Molecular Plant2022，15，3： | 8 |
| 11 | Construction of heparin-based hydrogel incorporated with Cu_(5.4)O ultrasmall nanozymes for wound healing and inflammation inhibition显示文摘Excessive production of inflammatory chemokines and reactive oxygen species(ROS)can cause a feedback cycle of inflammation response that has a negative effect on cutaneous wound healing.The use of wound-dressing materials that simultaneously absorb chemokines and scavenge ROS constitutes a novel‘weeding and uprooting’treatment strategy for inflammatory conditions.In the present study,a composite hydrogel comprising an amine-functionalized star-shaped polyethylene glycol(starPEG)and heparin for chemokine sequestration as well as Cu_(5.4)O ultrasmall nanozymes for ROS scavenging(Cu_(5.4)O@Hep-PEG)was developed.The material effectively adsorbs the inflammatory chemokines monocyte chemoattractant protein-1 and interleukin-8,decreasing the migratory activity of macrophages and neutrophils.Furthermore,it scavenges the ROS in wound fluids to mitigate oxidative stress,and the sustained release of Cu_(5.4)O promotes angiogenesis.In acute wounds and impaired-healing wounds(diabetic wounds),Cu_(5.4)O@Hep-PEG hydrogels outperform the standard-of-care product Promogram®in terms of inflammation reduction,increased epidermis regeneration,vascularization,and wound closure. | Yuan Peng Danfeng He Xin Ge Yifei Lu Yuanhao Chai Yixin Zhang Zhengwei Mao Gaoxing Luo Jun Deng Yan Zhang | 2021 | Bioactive Materials2021，6，10： | 7 |
| 12 | Harnessing Vis-NIR broad spectrum for photocatalytic CO2 reduction over carbon quantum dots-decorated ultrathin Bi2WO6 nanosheets显示文摘到充满精力的烃燃料的公司 2 的 photocatalytic 减小是探讨附随地的全球温暖和逼近的精力危机的一个有希望、持续的方法。然而,存在光催化剂的一个广阔多数仅仅能够利用紫外(紫外) 或 / 并且可见的光(力) ,而在红外线附近(NIR ) 区域仍然保持未经勘探。在这研究,碳量点(CQD ) 装饰了 ultrathin 双性人 2 WO 6 nanosheets (UBW ) 被表明是为在 Vis-NIR 宽广光谱上的公司 2 光致还原的有效光催化剂。CQDs/UBW 混合 nanocomposites 的合成过程是高度灵巧的,是引人注目的,包含帮助的一个壶 hexadecyltrimethylammonium 溴化物(CTAB ) 热水的过程。在可见轻照耀下面,优化 1CQDs/UBW (1 个 wt.% CQD 内容) 在太古的双性人 24 生产的显著 9.5 褶层和 3.1 褶层改进 > WO 6 nanoplatelets (PBW ) 和赤裸的 UBW 分别地。更重要地, CQDs/UBW 的 photocatalytic 应答成功地被扩大到 NIR 区域,没有包含任何稀土元素或高贵金属,它被完成。减小能被归因于 synergistic 的 驾驶NIR 的公司2的实现(i)完成 ultrathin nanostructures 并且高度暴露了{ 001 } UBW 的活跃方面,( ii ) UBW 和 CQD 的优秀光谱的联合,在 UBW 能由 CQD 的起来变换的光致发光是激动的的地方,并且( iii ) CQD 到的撤退电子的性质套住 photogenerated 电子并且延迟充电搬运人的再结合。 | Xin Ying Kong Wen Liang Tan Boon-Junn Ng Siang-Piao Chai Abdul Rahman Mohamed | 2017 | Nano Research2017，10，5： | 7 |
| 13 | Shape-Controlled Synthesis of Octahedral α-NaYF_(4) and Its Rare Earth Doped Submicrometer Particles in Acetic Acid显示文摘Submicrometer sized pure cubic phase,Eu^(3+)doped,and Yb^(3+)/Er^(3+)co-dopedα-NaYF4 particles with octahedral morphology have been prepared in acetic acid.The acetate anion plays a critical role in the formation of such symmetric octahedral structures through its selective adsorption on the(111)faces of the products.The size of the as-prepared octahedra can be tuned by varying the amount of sodium acetate added to the acetic acid.A possible formation mechanism for these octahedra has been proposed.The dopedα-NaYF_(4) octahedral submicrometer particles show down-conversion and up-conversion photoluminescence typical of these materials. | Li Gao Xin Ge Zhanli Chai Guohai Xu Xin Wang Cheng Wang | 2009 | Nano Research2009，2，7： | 6 |
| 14 | Comparative analysis of essential oils found in Rhizomes Curcumae and Radix Curcumae by gas chromatography-mass spectrometry显示文摘A comparison of the volatile compounds in Rhizomes Curcumae (Ezhu) and Radix Curcumae (Yujin) was undertaken using gas chromatography-mass spectrometry (GC-MS).Ultrasonic extraction and GC-MS methods were developed for the simultaneous determination of five sesquiterpenes,namely,a-pinene,b-elemene,curcumol,germacrone and curdione,in Ezhu and Yunjin.Good linearity (r40.999) and high inter-day precision were observed over the investigated concentration ranges.The validated method was successfully used for the simultaneous determination of five sesquiterpenes in Ezhu and Yujin.The quantitative method can be effectively used to evaluate and monitor the quality of Chinese curcuma in clinical use. | Di-Ya Lu Yan Cao Ling Li Zhen-Yu Zhu Xin Dong Hai Zhang Yi-Feng Chai Zi-Yang Lou | 2011 | Journal of Pharmaceutical Analysis2011，1，3： | 5 |
| 15 | UPLC-Q-TOF/MS based metabolomic profiling of serum and urine of hyperlipidemic rats induced by high fat diet显示文摘Hyperlipidemia is considered to be a high lipid level in blood,can induce metabolic disorders and dysfunctions of the body,and results in some severe complications.Therefore,hunting for some metabolite markers and clarifying the metabolic pathways in vivo will be an important strategy in the treatment and prevention of hyperlipidemia.In this study,a rat model of hyperlipidemia was constructed according to histopathological data and biochemical parameters,and the metabolites of serum and urine were analyzed by UPLC-Q-TOF/MS.Combining pattern recognition and statistical analysis.19 candidate biomarkers were screened and identified.These changed metabolites indicated that during the development and progression of hyperlipidemia,energy metabolism,lipid metabolism,amino acid metabolism and nucleotide metabolism were mainly disturbed,which are reported to be closely related to diabetes,cardiovascular diseases,etc.This study demonstrated that a UPLC-Q-TOF/MS based metabolomic approach is useful to profile the alternation of endogenous metabolites of hyperlipidemia. | Qiong Wu Hai Zhang Xin Dong Xiao-Fei Chen Zhen-Yu Zhu Zhan-Ying Hong Yi-Feng Chai | 2014 | Journal of Pharmaceutical Analysis2014，4，6： | 5 |
| 16 | Over-expression of aldehyde dehydrogenase-2 protects against H2O2-induced oxidative damage and apoptosis in peripheral blood mononuclear cells显示文摘 | Xiu-ying HU Qin FANG Ji-shi WANG Jian-qiong XIE Bai-sheng CHAI Fang-qiong LI Xin CUI Yuan YANG | 2011 | Acta Pharmacologica Sinica2011，32，2： | 5 |
| 17 | Risk factors for corticosteroid insufficiency during the sub-acute phase of acute traumatic brain injury显示文摘Hypothalamic-pituitary-adrenal axis dysfunction may lead to the occurrence of critical illness-related corticosteroid insufficiency.Critical illness-related corticosteroid insufficiency can easily occur after traumatic brain injury,but few studies have examined this occurrence.A multicenter,prospective,cohort study was performed to evaluate the function of the hypothalamic-pituitary-adrenal axis and the incidence of critical illness-related corticosteroid insufficiency during the sub-acute phase of traumatic brain injury.One hundred and forty patients with acute traumatic brain injury were enrolled from the neurosurgical departments of three tertiary-level hospitals in China,and the critical illness-related corticosteroid insufficiency incidence,critical-illness-related corticosteroid insufficiency-related risk factors,complications,and 28-day mortality among these patients was recorded.Critical illness-related corticosteroid insufficiency was diagnosed in patients with plasma total cortisol levels less than 10μg/dL(275.9 nM)on post-injury day 4 or when serum cortisol was insufficiently suppressed(less than 50%)during a dexamethasone suppression test on post-injury day 5.The results demonstrated that critical illness-related corticosteroid insufficiency occurred during the sub-acute phase of traumatic brain injury in 5.6%of patients with mild injury,22.5%of patients with moderate injury,and 52.2%of patients with severe injury.Traumatic brain injury-induced critical illness-related corticosteroid insufficiency was strongly correlated to injury severity during the sub-acute stage of traumatic brain injury.Traumatic brain injury patients with critical illness-related corticosteroid insufficiency frequently presented with hemorrhagic cerebral contusions,diffuse axonal injury,brain herniation,and hypotension.Differences in the incidence of hospital-acquired pneumonia,gastrointestinal bleeding,and 28-day mortality were observed between patients with and without critical illness-related corticosteroid insufficiency during the sub-acute phase of traumatic brain injury.Hypotension,brain-injury severity,and the types of traumatic brain injury were independent risk factors for traumatic brain injury-induced critical illness-related corticosteroid insufficiency.These findings indicate that critical illness-related corticosteroid insufficiency is common during the sub-acute phase of traumatic brain injury and is strongly associated with poor prognosis.The dexamethasone suppression test is a practical assay for the evaluation of hypothalamic-pituitary-adrenal axis function and for the diagnosis of critical illness-related corticosteroid insufficiency in patients with traumatic brain injury,especially those with hypotension,hemorrhagic cerebral contusions,diffuse axonal injury,and brain herniation.Sub-acute infection of acute traumatic brain injury may be an important factor associated with the occurrence and development of critical illness-related corticosteroid insufficiency.This study protocol was approved by the Ethics Committee of General Hospital of Tianjin Medical University,China in December 2011(approval No.201189). | Xin Chen Yan Chai Shao-Bo Wang Jia-Chong Wang Shu-Yuan Yue Rong-Cai Jiang Jian-Ning Zhang | 2020 | Neural Regeneration Research2020，15，7： | 4 |
| 18 | Clinical management and survival outcomes of patients withdifferent molecular subtypes of diffuse gliomas in China(2011–2017):a multicenter retrospective study from CGGA显示文摘Objective:We aimed to summarize the clinicopathological characteristics and prognostic features of various molecular subtypes of diffuse gliomas(DGs)in the Chinese population.Methods:In total,1,418 patients diagnosed with DG between 2011 and 2017 were classified into 5 molecular subtypes according to the 2016 WHO classification of central nervous system tumors.The IDH mutation status was determined by immunohistochemistry and/or DNA sequencing,and 1p/19q codeletion was detected with fluorescence in situ hybridization.The median clinical follow-up time was 1,076 days.T-tests and chi-square tests were used to compare clinicopathological characteristics.Kaplan‒Meier and Cox regression methods were used to evaluate prognostic factors.Results:Our cohort included 15.5%lower-grade gliomas,IDH-mutant and 1p/19q-codeleted(LGG-IDHm-1p/19q);18.1%lowergrade gliomas,IDH-mutant(LGG-IDHm);13.1%lower-grade gliomas,IDH-wildtype(LGG-IDHwt);36.1%glioblastoma,IDHwildtype(GBM-IDHwt);and 17.2%glioblastoma,IDH-mutant(GBM-IDHm).Approximately 63.3%of the enrolled primary gliomas,and the median overall survival times for LGG-IDHm,LGG-IDHwt,GBM-IDHwt,and GBM-IDHm subtypes were 75.97,34.47,11.57,and 15.17 months,respectively.The 5-year survival rate of LGG-IDHm-1p/19q was 76.54%.We observed a significant association between high resection rate and favorable survival outcomes across all subtypes of primary tumors.We also observed a significant role of chemotherapy in prolonging overall survival for GBM-IDHwt and GBM-IDHm,and in prolonging post-relapse survival for the 2 recurrent GBM subtypes.Conclusions:By controlling for molecular subtypes,we found that resection rate and chemotherapy were 2 prognostic factors associated with survival outcomes in a Chinese cohort with DG. | Kenan Zhang Xing Liu Guanzhang Li Xin Chang Shouwei Li Jing Chen Zheng Zhao Jiguang Wang Tao Jiang Ruichao Chai | 2022 | Cancer Biology & Medicine2022，19，10： | 4 |
| 19 | Ectopic expression of VRT-A2 underlies the origin of Triticum polonicum and Triticum petropavlovskyi with long outer glumes and grains显示文摘Polish wheat (Triticum polonicum) is a unique tetraploid wheat species characterized by an elongated outer glume. The genetic control of the long-glume trait by a single semi-dominant locus, P1 (from Polish wheat), was established more than 100 years ago, but the underlying causal gene and molecular nature remain elusive. Here, we report the isolation of VRT-A2, encoding an SVP-clade MADS-box transcription factor, as the P1 candidate gene. Genetic evidence suggests that in T. polonicum, a naturally occurring sequence rearrangement in the intron-1 region of VRT-A2 leads to ectopic expression of VRT-A2 in floral organs where the long-glume phenotype appears. Interestingly, we found that the intron-1 region is a key ON/OFF molecular switch for VRT-A2 expression, not only because it recruits transcriptional repressors, but also because it confers intron-mediated transcriptional enhancement. Genotypic analyses using wheat accessions indicated that the P1 locus is likely derived from a single natural mutation in tetraploid wheat, which was subsequently inherited by hexaploid T. petropavlovskyi. Taken together, our findings highlight the promoter-proximal intron variation as a molecular basis for phenotypic differentiation, and thus species formation in Triticum plants. | Jing Liu Zhaoyan Chen Zhihui Wang Zhaoheng Zhang Xiaoming Xie Zihao Wang Lingling Chai Long Song Xuejiao Cheng Man Feng Xiaobo Wang Yanhong Liu Zhaorong Hu Jiewen Xing Zhenqi Su Huiru Peng Mingming Xin Yingyin Yao Weilong Guo Qixin Sun Jie Liu Zhongfu Ni | 2021 | Molecular Plant2021，14，9： | 4 |
| 20 | Using cell membrane chromatography and HPLC-TOF/MS method for in vivo study of active components from roots of Aconitum carmichaeli显示文摘An offline two-dimensional system combining a rat cardiac muscle cell membrane chromatography time-of-flight mass spectrometry(CMC-TOF/MS)with a high performance liquid chromatography time-of-flight mass spectrometry(HPLC-TOF/MS)was established for investigating the parent components and metabolites in rat urine samples after administration of the roots of Aconitum carmichaeli.On the basis of the analysis of the first dimension,retention components of the urine sample were collected into 30 fractions(one fraction per minute).Then offline analysis of the second dimension was carried out.34 compounds including 24 parent alkaloids and 10 potential metabolites were identified from the dosed rat urine,and then binding affinities of different compounds on cell membranes were compared and influences of some functional groups on activity were estimated with the semi-quantification and curve fitting method.As a result,binding affinities decreased along with the process of deacylation,debenzoylation and demethylation,which may be related to the alleviation of toxicity in the procedure of herb processing or metabolism.Moreover,some minor components in rat urine(Songorine,14-benzoylneoline,Deoxyaconitine,etc.)exerted relatively strong affinity on cell membranes are worth exploring.The results delivered by the system suggest that the CMC can be applied to in vivo study. | Yan Cao Xiao- Fei Chen Di- Ya Lu Xin Dong Guo-Qing Zhang Yi- Feng Chai | 2011 | Journal of Pharmaceutical Analysis2011，1，2： | 4 |
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