|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Probiotic metabolites from Bacillus coagulans GanedenBC30^(TM) support maturation of antigen-presenting cells in vitro显示文摘AIM:To study the effects of probiotic metabolites on maturation stage of antigen-presenting immune cells.METHODS:Ganeden Bacillus coagulans 30(GBC30) bacterial cultures in log phase were used to isolate the secreted metabolite(MET) fraction.A second fraction was made to generate a crude cell-wall-enriched fraction,by centrifugation and lysis,followed by washing.A preparation of MET was subjected to size exclusion centrifugation,generating three fractions:< 3 kDa,3-30 kDa,and 30-200 kDa and activities were tested in comparison to crude MET and cell wall in primary cultures of human peripheral blood mononuclear cell(PBMC) as a source of antigen-presenting mononuclear phagocytes.The maturation status of mononuclear phagocytes was evaluated by staining with monoclonal antibodies towards CD14,CD16,CD80 and CD86 and analyzed by flow cytometry.RESULTS:Treatment of PBMC with MET supported maturation of mononuclear phagocytes toward both macrophage and dendritic cell phenotypes.The biological activity unique to the metabolites included a reduction of CD14+ CD16+ pro-inflammatory cells,and this property was associated with the high molecular weight metabolite fraction.Changes were also seen for the dendritic cell maturation markers CD80 and CD86.On CD14dim cells,an increase in both CD80 and CD86 expression was seen,in contrast to a selective increase in CD86 expression on CD14bright cells.The co-expression of CD80 and CD86 indicates effective antigen presentation to T cells and support of T helper cell differentiation.The selective expression of CD86 in the absence of CD80 points to a role in generating T regulatory cells.CONCLUSION:The data show that a primary mechanism of action of GBC30 metabolites involves support of more mature phenotypes of antigen-presenting cells,important for immunological decision-making. | Kathleen F Benson Kimberlee A Redman Steve G Carter David Keller Sean Farmer John R Endres Gitte S Jensen | 2012 | World Journal of Gastroenterology2012，18，16： | 8 |
| 2 | Prognostically relevant gene signatures of high-grade serous ovarian carcinoma显示文摘 | Verhaak Roel G W Tamayo Pablo Yang Ji-Yeon Hubbard Diana Zhang Hailei Creighton Chad J Fereday Sian Lawrence Michael Carter Scott L Mermel Craig H Kostic Aleksandar D Etemadmoghadam Dariush Saksena Gordon Cibulskis Kristian Duraisamy Sekhar | 2013 | Journal of Clinical Investigation2013，，1： | 2 |
| 3 | Modulation of statin- activated shedding of Alzheimer APP ectodomain by ROCK 显示文摘 | Pedrini S Carter TL Prendergast G | 2005 | PLoS Med2005，2，1： | 1 |
| 4 | VEGF polymor-phisms are associated with severity of diabetic retinopathy显示文摘 | Churchill A J Carter J G Rasmden C | 2008 | Invest Ophthalmol Vis Sci2008，49，8： | 1 |
| 5 | Pathology of acute and chronic coronary stenting in humans显示文摘 | Farb A Sangiorgi G Carter AJ | 1999 | Circulation1999，99，： | 1 |
| 6 | The prevalence of nascent entrepreneurs in the United States: evidence from the panel study of entrepreneurial dynandler显示文摘 | Reynolds P D N M Carter W B Gartner P G Greene | 2004 | Small Business Economics2004，23，： | 1 |
| 7 | 显示文摘 | Carter G M Hryniewicz J V Thakur M K | 1986 | Appl Phys Lett1986，48，： | 1 |
| 8 | Polarization- insensitive wavelength conversion by FWM in a highly nonlinear PCF of polarization-scrambled 10-Gb/s RZ-OOK and RZ-DPSK signals 显示文摘 | ASTAR W LENIHAN A S CARTER G M | 2007 | IEEE Photonics Technology Letters2007，19，20： | 1 |
| 9 | Time delay estimation for passive sonar signal processing显示文摘 | Carter G C | 1981 | IEEE Transactions on Acoustics Speech Signal Processing1981，29，3： | 1 |
| 10 | Freezing effects on aggregate stability affected by texture, mineralogy, and organic matter显示文摘 | Lehrsch G A Sojka R E Carter D L | 1991 | Soil Science Society of America Journal1991，55，： | 1 |
| 11 | Prevalence and impact of gastroesophageal reflux in adolescents with asthma: a population-based study 显示文摘 | Debley J S Carter E R Redding G J | 2006 | Pediatr Pulmonol2006，41，5： | 1 |
| 12 | Combind analysis of post fracturing performance and pressure buildup data for evaluating an MHF gas well显示文摘 | Bostic J N Agarwal R G Carter R D | 1979 | SPE1979，8280，： | 1 |
| 13 | Storage of sediment-associated nutrients and contaminants in river channel and floodplain systems显示文摘 | Walling D E Owens P N Carter J Leeks G J L Lewis S Meharg A A and Wright J | 2003 | Applied Geochemistry2003，18，： | 1 |
| 14 | Replacement of fish oil with sunflower oil in feeds for Atlantic salmon (Salmo salar L):effect on growth performance,tissue fatty acid composition and disease resistance显示文摘 | BRANSDEN M P CARTER C G NICHOLS P D | 2003 | Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology2003，135，4： | 1 |
| 15 | Bisphosphonates and avascular necrosis of the jaw: a possible association 显示文摘 | Carter G Goss AN Doecke C | 2005 | Med J Aust2005，182，8： | 1 |
| 16 | The relationship between student beliefs about mathematics显示文摘 | Carter G Norwood K S | 1997 | School Science and Mathematics1997，97，： | 1 |
| 17 | Geographic Information Systems for Geoscientists Modelling with GIS, Pergamon 显示文摘 | Bonham - Carter G F | 1994 | Ontario1994，，： | 1 |
| 18 | Macrophage pacification reduces rodent pancreatitis-induced hepatocellular injury through down-regulation of hepatic tumor necrosis factor alpha and interleukin-1beta 显示文摘 | Denham W Carter G | 1998 | Hepat ology1998，28，5： | 1 |
| 19 | The generalized correlation method for estimation of time delay 显示文摘 | Knapp C H Carter G C | 1976 | IEEE Trans on ASSP1976，24，4： | 1 |
| 20 | The illusion of environmentaI iodine deficiency 显示文摘 | Stewart A G Carter J Parker A Ailoway B J | 2003 | Environmental Geochemistry and Health2003，25，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员