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| 1 | Nutrient supplemented serum-free medium increases cardiomyogenesis efficiency of human pluripotent stem cells显示文摘AIM: To development of an improved p38 MAPK inhibitor-based serum-free medium for embryoid body cardiomyocyte differentiation of human pluripotent stem cells. METHODS: Human embryonic stem cells (hESC) differentiated to cardiomyocytes (CM) using a p38 MAPK inhibitor (SB203580) based serum-free medium (SB media). Nutrient supplements known to increase cell viability were added to SB medium. The ability of these supplements to improve cardiomyogenesis was evaluated by measurements of cell viability, total cell count, and the expression of cardiac markers via flow cytometry. An improved medium containing Soy hydrolysate (HySoy) and bovine serum albumin (BSA) (SupSB media) was developed and tested on 2 additional cell lines (H1 and Siu-hiPSC). Characterization of the cardiomyocytes was done by immunohistochemistry, electrophysiology and quantitative real-time reverse transcriptionpolymerase chain reaction. RESULTS: hESC cell line, HES-3, differentiating in SB medium for 16 d resulted in a cardiomyocyte yield of 0.07 ± 0.03 CM/hESC. A new medium (SupSB media) was developed with the addition of HySoy and BSA to SB medium. This medium resulted in 2.6 fold increase in cardiomyocyte yield (0.21 ± 0.08 CM/hESC). The robustness of SupSB medium was further demonstrated using two additional pluripotent cell lines (H1, hESC and Siu1, hiPSC), showing a 15 and 9 fold increase in cardiomyocyte yield respectively. The age (passage number) of the pluripotent cells did not affect the cardiomyocyte yields. Embryoid body (EB) cardiomyocytes formed in SupSB medium expressed canonical cardiac markers (sarcomeric α-actinin, myosin heavy chain and troponin-T) and demonstrated all three major phenotypes: nodal-, atrial- and ventricular-like. Electrophysiological characteristics (maximum diastolic potentials and action potential durations) of cardiomyocytes derived from SB and SupSB media were similar. CONCLUSION: The nutrient supplementation (HySoy and BSA) leads to increase in cell viability, cell yield and cardiac marker expression during cardiomyocyte differentiation, translating to an overall increase in cardiomyocyte yield. | Sherwin Ting Marti Lecina Yau-Chi Chan Hung Fat Tse Shaul Reuveny Steve KW Oh | 2013 | World Journal of Stem Cells2013，5，3： | 4 |
| 2 | Outcome and prognostic indicators of diffuse proliferative lupus glomerulonephritis treated with sequential oral cyclophosphamide and azathiop rine显示文摘 | Mok CC Ho CT Chan KW | 2002 | Arthritis Rheum2002，46，： | 1 |
| 3 | Clofarabine, a novel nucleoside analog,is active in pediatric patients with advanced leukemia显示文摘 | JEHA S GANDHI V CHAN KW | 2004 | Blood2004，103，3： | 1 |
| 4 | Volar fixation of dorsally displaced distal radial fracture using locking compression plate显示文摘 | Wong KK Chan KW Kwok TK | | 0，，： | 1 |
| 5 | Improvement of heavy metal stress and toxicity assays by coupling a transgenic reporter in a mutant nematode strain 显示文摘 | Chu KW Chan SKW Chow KL | 2005 | Aquati Toxicol2005，74，4： | 1 |
| 6 | Scan-direeted unilateral neck exphoralion for primary hyperparathyroidism: eight-year results from a regional hospital显示文摘 | Wong SW Chan KW Paulose NM | 2009 | Hong Kong Med J2009，15，2： | 1 |
| 7 | RF heating of implanted spinal fusion stimulator during magnetic resonance imaging显示文摘 | Chou CK Mcdougall JA Chan KW | 1997 | IEEE Trans Biomed Eng1997，44，5： | 1 |
| 8 | Blocking BRE expression in Leydig cells inhibits steroidogenesis by down-regulating 3 betahydrnxysteroid dehydrogenase 显示文摘 | Miao J Chan KW Chen GG | 2005 | J Endocrinol2005，185，3： | 1 |
| 9 | Volar fixation of dorsally displaced distal radial fracture using locking compression plate显示文摘 | Wong KK Chan KW Kwok TK | 2005 | J Orthop Surg (Hong Kong)2005，13，15： | 1 |
| 10 | Identification and characterization of tumorigenic liver cancer stem/progenitor cells显示文摘 | MA S CHAN KW HU L | 2007 | Gastroenterology2007，132，： | 1 |
| 11 | Surgi- cal morbidity and mortality in obturator hernia:A 10-year retrospective risk factor evaluation 显示文摘 | Chan KV Chan CK Yau KW et at | 2014 | Hernia2014，18，3： | 1 |
| 12 | A- queous humor levels of vascular endothelial growth factor and pigment epithelium derived factor in polypoidal choroidal vascu- lopathy and choroidal neovascularization显示文摘 | Tong JP Chan WN Liu DT Lai TY Choy KW Pang CP | 2006 | Am J Ophthal- mol2006，141，3： | 1 |
| 13 | Vo la r fix a tio no f do rsally displaced distal radial fracture using locking compression plate显示文摘 | Wong KK Chan KW Kw ok TK | 2005 | J Ortho p Surg (Hong Kong)2005，13，2： | 1 |
| 14 | Adenovirus-mediated down-regulation of X-linked inhibitor of apoptosis protein inhibits colon cancer显示文摘 | Dai Y Qiao L Chan KW | 2009 | Mol Cancer Ther2009，8，9： | 1 |
| 15 | Management of massive and irreparable rotator cuff tears显示文摘 | Neri BR Chan KW Kwon YW | 2009 | J Shoulder Elbow Surg2009，18，5： | 1 |
| 16 | Nuclear and cytoplasmic Bcl-2 expression in endometrial hyperplasia and adenocarcinoma显示文摘 | Chan KW Mole MM Levison DA | 1995 | J Pathol1995，177，： | 1 |
| 17 | Two-port laparoscopic chole-cystectomy :initial results of a modified technique显示文摘 | Poon CM Chan KW Ko CW | 2002 | J Laparoen-dosc Adv Surg Tech A2002，12，4： | 1 |
| 18 | Chinese viewer's perception of informative and emotional advertising 显示文摘 | Chan K KW | 1996 | International Journal of Advertising1996，15，： | 1 |
| 19 | Infliximab for GVHD therapy in children 显示文摘 | Sleight BS Chan KW Braun TM | 2007 | Bone Marrow Transplant2007，40，5： | 1 |
| 20 | Breast pathology in complications associated with polyacrylamide hydrogel (PAAG) mammoplasty显示文摘 | LEUNG KM YEOH GP CHAN KW | 2007 | Hong Kong Med J2007，13，2： | 1 |
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